FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s16.352000000d7d0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s16.352000000d7d0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTTATGAGGCAATAGCGAAAAACTGCATTTGACTGAGAGTTATTTCA10.9433962264150944No Hit
AGCTCATTAGGGTTAGCCTCGGTACGGTCAGGCATCCACGGCGCTTTAAA10.9433962264150944No Hit
TATTACTCCGGAGGCAGCTCACGAACTGGATACTAGCAATCTCTTTTTGA10.9433962264150944No Hit
ACGTCCACCACGCAGAGATCCCAGCTAACAGCAACCACAGCAAGGACGAC10.9433962264150944No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA10.9433962264150944No Hit
AAAGCGCCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGAAGTAGGCGG10.9433962264150944No Hit
AGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTTG10.9433962264150944No Hit
ATATTGCTCAAGGAGCCGCGATTTGCGCAGATTGCTAGGCAGATGTCGGA10.9433962264150944No Hit
CATTGGATGACAAACTAACGTGCAGCCAGGAATTATCGATTCAGATCCCA10.9433962264150944No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA10.9433962264150944No Hit
CTGTACGAGACGGAACGACTTGTGCAAGAGCTAACCAAGTGCGGCATCGA10.9433962264150944No Hit
GATATAGACAAGTCAACGGAAACTCTTGAAAACATTTTAGTTTCGGCTGC10.9433962264150944No Hit
CTCTACTGCTATAGATGACCGAGAAGGATACCGTCTCGATGGCTACCGTG10.9433962264150944No Hit
CCCAAGAAGGCTGCCGAGCCCGCCAAGGAGTTGGACGCCGCCGATGAGGC10.9433962264150944No Hit
ACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGC10.9433962264150944No Hit
GTGTTATTATTGTATTAAAGTTTCCTTTGCCATTTACCACACACAACAAC10.9433962264150944No Hit
GTTCCATGTCGTACCCGTTATTTTCCATCATTTCTTTCGTGATGGGGTAG10.9433962264150944No Hit
GAGACACACACAACTGGCAGCCAGCGATCAATGTGATCAATCGCGCTTCA10.9433962264150944No Hit
CTGAAAGAGCGATGTCCACTTCCGGGTATTTTTTTTGCAAGTAATAGGCA10.9433962264150944No Hit
CAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAA10.9433962264150944No Hit
GTTAGCCACAATGATCTTCTTGGAGGTCTTGCCAGACTGCGATCCATAAG10.9433962264150944No Hit
CCCCACCAACCAAATCCTTCCATCCCAACCAACCCACTTCCCACCCAAAC10.9433962264150944No Hit
GATCCATGCTGATCCAATTACCACATTTTCTTGATTTGGAATCGCTCTCG10.9433962264150944No Hit
CAACAGCAGCAACACGTTAACAACAGCAAAAACAACAAAACAAAGCAAAT10.9433962264150944No Hit
ACCCTGTACATTGTTCACCGTTGAAACTGAGCCTGATGGCGCGGAGTTGA10.9433962264150944No Hit
ACTTCATTGTTGGCATCAATAACAAGTTGCTTCATGTCTTCATAGCTGGG10.9433962264150944No Hit
TTACTGTAGCCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTC10.9433962264150944No Hit
CTGGCAAACTGAACGCATTAACAACAACAACAAAAAGAAGGATACTTTAA10.9433962264150944No Hit
GCTCTGATTCAGCTTTTAGTTGTGGTTCTGTTGCTCTTTTTGATCCGGGG10.9433962264150944No Hit
ATGGAGAACCTCGCGTCGGCCAAAACGTGTCTTAAACTGCTGCAGGTTAT10.9433962264150944No Hit
ACACCAGAAGCAGCATCAGTGACGACATTAGAAATATCCTTTGCAGTAGC10.9433962264150944No Hit
TCCATGGGGTTTCGACGACGGCGGCGAGGAGGAGAAGGCGGCATCCACCC10.9433962264150944No Hit
ATTTCAAGTTGTGAATTGTGAAAAATGGATCAGATAGCGATTATAGGATG10.9433962264150944No Hit
CCAATCCACCAAGCTGAGTAGTTGCAATTACGGCGCGGCGGAAAACTGTT10.9433962264150944No Hit
CGACAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCGGAGGCGG10.9433962264150944No Hit
TGTCTCCCTTTTGGCCAGGAGTGCCGGGACGACCAGCGTCTCCCTTTTGT10.9433962264150944No Hit
GGGCGGCCCACCGGTTGCGCCGCGGACGCAGACCGTGATATGGTAGGCAG10.9433962264150944No Hit
CGTGGAGATGTTTCCGATTTTCCTCGGCCGCGAGGCACCCCATCAGCTCA10.9433962264150944No Hit
GCCATCCATTGGGCTGGCCCAAGAACGGTTTCCCCGGACCCCAGGGACAC10.9433962264150944No Hit
CGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCA10.9433962264150944No Hit
ACCCACTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT10.9433962264150944No Hit
GATTTTGGGTGCCGGCTATTTTGTTCTAGTTTTTCAGCTCAATCTAATCA10.9433962264150944No Hit
TATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCA10.9433962264150944No Hit
CCCTTATTGTGGGAGGAGGTGCGTTCGGATTAGGTGGAAACAGGATGCAG10.9433962264150944No Hit
GAACAGGAAGTGGTCGTCGATCAGCTGCTGCTGGACGGCCTTCTCCATGC10.9433962264150944No Hit
ACCTAATAGTAACAAACAAAAGCAAACTCACTGAAGAACATAAAGTCCAA10.9433962264150944No Hit
CGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTAATCATGTTTCAGACTTT10.9433962264150944No Hit
GAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGA10.9433962264150944No Hit
CTCTATTATTGTACTTTTTGCACAACGCTCTGCCTTTTTCCAGGAGTCAT10.9433962264150944No Hit
GGCAAATGGCGAGCGACAGAGAGGCAAGAGGGCTGCGCACACAAGTACAA10.9433962264150944No Hit
GCATCACCCATGCCTACAGTATTGTTATCGGTAGCAAGCACATCACCTTG10.9433962264150944No Hit
CAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAAC10.9433962264150944No Hit
ATCCAAGCGCTGGCTTTATAGGCTGTCTCACTTCTGTCTCTTATACACAT10.9433962264150944No Hit
CCCTCTGACCCCTTCCTTGCCTCTTCCTGGCTCCTGACCTGTCTCTTATA10.9433962264150944No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGA10.9433962264150944No Hit
GTTGTAAGATCTAAAGAAATCTAAAGCGAGCTTTATGTATCAAGGCTATT10.9433962264150944No Hit
AGCTAGAACAGCTCCCCAAAGGACACAGGTGGTAGTGGCAAAGCCAAGAC10.9433962264150944No Hit
GTCGTAGGCACGTTGCAATTCTGATTTATGGTTCAGCACGCTCTTTCCCA10.9433962264150944No Hit
TAGTAGCGATATCGGTTCACCGGCAGACCGGCGTGCTCGAAAATGGCCAC10.9433962264150944No Hit
ACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATC10.9433962264150944No Hit
CTGTGAAGTGAGCTGATGTGTTGTCGTTGTTACAGGGCTGCTGCTGCAAC10.9433962264150944No Hit
TTTAAAGCGACGTGGATGCCTGACCGTACCGAAGCTAACACTAATGAGCT10.9433962264150944No Hit
CCTGCTTTATCAAGATAATTTTTCGACTCATCAGAAATATCCGAAAGTGT10.9433962264150944No Hit
ATGAATCGGTGCTAGATACGATATAGACAACTGATTGCTTGCTGGCGATA10.9433962264150944No Hit
GCCTTGTAACAGTGCCCTCAAAATCGAGCTTTTTAGCCAGCACGCACGCG10.9433962264150944No Hit
TTTTATTAACTCTTCATAAAGAATTTTAAAACTTTATTAGGACTGTCTCT10.9433962264150944No Hit
ACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGG10.9433962264150944No Hit
ACAATATCCGTCTCCGGCTTCTGATCCTCTCCTTCGACGGCTGAGGCTGC10.9433962264150944No Hit
TCCATACTGATTGAATTGCGGCTGCACGGCATTGCTGCCGGTGAAATTCA10.9433962264150944No Hit
GAAGTACTTTACCAGCTTTAGCCATAGCACCAGAAACAAAACTAGGGACG10.9433962264150944No Hit
GTAGCAGTTGTGGGCCAAAGCAGTCAGCACGTAAAGCGTGAGGAAAAGCA10.9433962264150944No Hit
GGATAAAGGGGCGCGGGGTTTGTGAGCAGTACAGGGATAGTGGCAATAGG10.9433962264150944No Hit
CGACTCACACTAAAGTACTGAAGGCAATTGGCGTGTGAAGCATTCGCCTT10.9433962264150944No Hit
TAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAG10.9433962264150944No Hit
GTAGCTCCATCTCTTTCGAACCCAACTGACCCCCTCCTTCTCACACATCC10.9433962264150944No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG10.9433962264150944No Hit
GCCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTATGGTTAG10.9433962264150944No Hit
ACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAG10.9433962264150944No Hit
GGCTGATTCAGCGTCGTCGATGTTGTTGTTGAAACATCCGGTGTTGGATG10.9433962264150944No Hit
TTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAG10.9433962264150944No Hit
ACACCAACCATAAAGATATATCAATGCCCACTTCTATACAAAGACAAGCA10.9433962264150944No Hit
TTGTTTATCTTACTGAAATCTTTGAATTGTCTGGGTAGTCTGCCAAATAC10.9433962264150944No Hit
TCTGTAAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTT10.9433962264150944No Hit
CAAAATGCCTATTAAAGAAACGCGGAGATTTGCTCGCCAGCTCTGCCCAC10.9433962264150944No Hit
GCCGCCAAAACGTCGGCTACAGTAACTTTTCCCAGCCTCAATCTCATCTC10.9433962264150944No Hit
GTTATGGAATTGGGATTTCTTGGGAGTTTGCCCGTGTTTGTGGCAAATAA10.9433962264150944No Hit
GCATAACCCAAGGGCTGTTCGGTGAATTCGATCAGTTTAATGCCACCGTC10.9433962264150944No Hit
TACTTGAACCGTGGGCCCGGCCCCGGACCGGAGTACTCCGACACGTCCGA10.9433962264150944No Hit
CATCACCATCGACGAGCCCCTGCCAAACTCGAACGATCGTATCATCACCA10.9433962264150944No Hit
GTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATATGGACCT10.9433962264150944No Hit
CTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAG10.9433962264150944No Hit
GTTCAATGTGATGTTGGCCAGATGTGCGGCCAACAGGGCATAGACGCCAC10.9433962264150944No Hit
AGCCTATACGGCGGCGGCGTTCTTTGGCCTGACAGCATTTTGCATCAACG10.9433962264150944No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATACGAGTCCTGT10.9433962264150944No Hit
TGGTGGAGCAGTTCTGGACGACATCAATAGCCTGACAGAAGTGACCATTT10.9433962264150944No Hit
CCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACA10.9433962264150944No Hit
CAGTCGCGGTTAACGAAACACGAAAAGTCGTCTCGACTCGCCTCGCGTCG10.9433962264150944No Hit
GGCATCCTCTGAACAGCCGACGCCCGCAAACGCAGCGTTGCATAAATTCA10.9433962264150944No Hit
TCACCGGGATCCGTTGTCTTTTCCACACGTATTCGGGTGTCTTGTACTAA10.9433962264150944No Hit
TACCAATATCAGCAATCAAAGCCCACACATATGCAGCAATTAGCTTGTAA10.9433962264150944No Hit
CAAGAGAGGCGGTGAACAGAGCTTGGGGCATAGAAACAAAATACACGGGG10.9433962264150944No Hit
CATATAATATATATGAATAATAGAAAATGAAGTGTTCATACTGTCTCTTA10.9433962264150944No Hit
TCTGCAAGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTACT10.9433962264150944No Hit
CGTCAACGCTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGT10.9433962264150944No Hit
TCCAAACACAACAAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9433962264150944No Hit
TCCTGAGACAATGGGAGAGTGCCAATAATCTGCGCCGCGTAGCCCTGCAA10.9433962264150944No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers