FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s12.352000000d7c91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s12.352000000d7c91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA50.28457598178713717No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT30.1707455890722823Illumina RNA PCR Primer (100% over 25bp)
GCTATCCTTAGTACGAGAGGACCGGGATGGACGCACCGCTGGTGCACCAG20.11383039271485487No Hit
ATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCAACTTAGAGTGCCCAA20.11383039271485487No Hit
GCCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGG20.11383039271485487No Hit
TGGTAGGCCTTTACCCTACCAACTAGCTAATGCGCCGCGAGGCTATCCAT20.11383039271485487No Hit
CCTATATTCTAGCTAGAGGCTTTTCTCGGCAGTGTGAAATCAACGACTCG20.11383039271485487No Hit
ACATGAAGCTGGAATCGCTAGTAATCGTAGATCAGCATGCTACGGTGAAT20.11383039271485487No Hit
GCTCATAAGGGGCATGATGATTTGACGTCATCCCCGCCTTCCTCCGGTTT20.11383039271485487No Hit
GCTTCATGTAGTCGAGTTGCAGACTACAATCCGAACTGAGAACAACTTTA20.11383039271485487No Hit
GTGTAAAGGCAGAAGGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGG20.11383039271485487No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC20.11383039271485487No Hit
ATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAGTTGTT20.11383039271485487No Hit
GTGTAAAGGCATAAGGGAGCTTGACTGCGAGACTTACAAGTCGAGCAGGG20.11383039271485487No Hit
GTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGC20.11383039271485487No Hit
GTATAGGGGCTGACACCTGCCCGGTGCTGGAAGGTTAAGTGGACGTGTTA20.11383039271485487No Hit
TAGCAAGACTGCCGGTGATAAACCGGAGGAAGGCGGGGATGACGTCAAAT20.11383039271485487No Hit
CCATAAAGTTGTTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGA20.11383039271485487No Hit
TAGCTAATCTCCCAAAGCCATTCTCAGTTCGGATTGCAGGCTGCAACTCG20.11383039271485487No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGGC20.11383039271485487No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT20.11383039271485487Illumina RNA PCR Primer (96% over 25bp)
GTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATGATTTG20.11383039271485487No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGCTCTCGGTGGTCGCCGT20.11383039271485487Illumina RNA PCR Primer (96% over 25bp)
GGACAATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAG20.11383039271485487No Hit
GTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGT20.11383039271485487No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)