FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s11.352000000d7c77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s11.352000000d7c77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9869290
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA375760.38073660820585875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGACG122200.037.4272467
GAGACGT116550.037.408088
AGACGTG120950.033.4557659
GGGAGAC151100.030.9915376
TTCCGAT168200.026.2874032
CCGATCT172200.025.180474
TCCGATC178950.024.7349153
CGATCTG67650.023.3075455
CGATCTT66350.021.6884215
CTTCCGA255500.020.0564731
CGGGAGA44450.018.1636625
CGATCTA41050.017.7012735
TCCGAAC31150.017.2284663
GACGTGT122700.016.68313410-11
ACGTGTG128150.016.08481410-11
AGACGTT23550.015.52876959
TCTCGGG41300.015.29943294-95
CGTGTGC134400.014.73618312-13
TCGCCGG46350.014.50375394-95
AGGGAGA125950.013.8386635
Produced by FastQC (version 0.11.2)