FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s10.352000000d7c5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s10.352000000d7c5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966793
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT115190.29038570956437604No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT69560.17535575967790606No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG64880.16355781609980657No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT64780.16330572328830872No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA64510.1626250726972645No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA61520.15508749763347873No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC61060.1539278707005886No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC55330.1394829526017617No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT53420.1346679799021527No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG52380.13204621466257502No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA49000.12352547763394763No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA48710.12279440848060386No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA46420.11702148309730304No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG44980.11339134661173397No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA44110.11119813915170264No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT43330.1092318152220194No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA42560.10729070057348593No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA42340.10673609638819066No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT40470.10202196081318084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT54700.046.6259964
TCCGATC57300.044.344563
CTTCCGA58850.043.6608281
TTCCGAT59650.042.6771622
TCTTCCG27600.024.2633745
TAGGACT65950.022.1807944
ATCTAGG67900.021.4038961
AGGACTA69100.020.8947285
CTAGGAC68050.020.798373
GACTACC67550.020.6715987
TATGCTT32850.020.3856665
TCTAGGA74300.020.1355232
GGACTAC72100.019.8940776
TATAGTA44450.019.8738672
TCTAGGG5300.019.71462
ATATAAG24400.019.2702142
CGATCTC30400.019.216435
GTATAGT43300.019.0854551
TATAAGA25250.018.4334163
TAATACT44400.018.3986784
Produced by FastQC (version 0.11.2)