FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s1.352000000d7b35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s1.352000000d7b35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15630248
Sequences flagged as poor quality0
Sequence length101
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA939460.6010525232868985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC633650.036.3893746
GAGACGT609750.035.0430378
GGAGACG647550.034.9117167
CGATCTG361750.033.280325
TTCCGAT948000.032.0569232
AGACGTG606950.032.0200239
TCCGAAC167500.031.925793
CCGATCT936350.031.7101884
TCCGATC977900.031.1981743
TGGGAGA256100.029.1144225
AGGGAGA311800.028.1325365
AGACGTT73950.025.368689
CGATCTT258550.024.5586765
GATCTGG168150.024.0927816
CAGGGAG179550.023.6995834
CGATCTA222550.023.303075
CTTCCGA1621800.022.4515461
CCGAACT142550.022.2549864
CGGGAGA206250.021.3223515
CTGGGAG180600.021.3002834
Produced by FastQC (version 0.11.2)