FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r2zt2wts86.352000000d502a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r2zt2wts86.352000000d502a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGACGCTGGCTACCCAGACGCTATTGTCATTATCGTACGTCGCTCG10.6802721088435374No Hit
CAATAGTCTCGCCAGAGGAATTTATCGGCAGTGTGAAATCAACGACTCGA10.6802721088435374No Hit
ATACTCACCCGACCCTCCAGACGAATAGAGAAGAGCACCCCGCACGCGGC10.6802721088435374No Hit
GAAGAGCACACACCGCACGAGATCGGAAACAGTCGCTTATACACATCTGA10.6802721088435374No Hit
CACCTGAGACGACGGGAGAGAAAAAGTTCCTGAAACTGGATGGATACAAA10.6802721088435374No Hit
CTTCTAAGCGCTCCACATGTCCTTGCGATCATGCTTCGACGCCCTTAGAA10.6802721088435374No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAAATCCCGGCG10.6802721088435374No Hit
GTCATGGGACACTCCGTCGGAGATACACGGATTGTTCAACCGCAACGTGG10.6802721088435374No Hit
GTTTCAGGAACTCTTTCACTCCCCTCCCGGGGTACTTTTCACCATTCCCT10.6802721088435374No Hit
CCTCAGGGGTCAAGGGAAAAAGTGCACATGGTGGATGCCTTGGCAGTCAT10.6802721088435374No Hit
CATCTGCCGCTGCCCGACGAATAGAGAGGTGTAGCCCTCGGTGGGCGCCG10.6802721088435374No Hit
CCTATGCGGTATTAGCACCCGTTTCCGGGTGTTATCCCCCTCTTATGGGC10.6802721088435374No Hit
CTAATACACATCTGACGCTGCCGCAGAATAGAGAGGATTCCACCCCGGTT10.6802721088435374No Hit
CTTAAACACATCTCACGCCCGAAAAAAAAAAAAAAATATAAAACACAAAA10.6802721088435374No Hit
TCAGGTCAGCAGAAACCAAGAAAGACGAGAGTAATAATACACGGATAGTA10.6802721088435374No Hit
GTTGTCGTCCGCTCTTTCGTTCTAATTTGGGTTTTGTTTGCTTTTCCTGT10.6802721088435374No Hit
GTTAGGTGCCTTTCAGCGCTCTTTCTCTGGCTTTTCCTAGCGGCCTCGAG10.6802721088435374No Hit
ACTCACAACAGACTCCAGCGACGAAAGGCAGTGCGCATAAAGCTGAGGTA10.6802721088435374No Hit
GATCTGCAGACGCCGTCAAATGGAGAAGCGCATACGCAGGATGCCTTGGC10.6802721088435374No Hit
ATACACATCTGACGCTGCCGACGAATAGAGCGGTGTCGAACGCGGAGGGC10.6802721088435374No Hit
CAGCAGGTCAACCATAAACCGCCCGGAACCCAGCCGCCCGAAGCAGTGCC10.6802721088435374No Hit
CGATTAGATATCCTGGTAGTCCGAGCAGTAAACGATGAGTGCTCAGTGTT10.6802721088435374No Hit
CAGCCGGGAGACGTGTGCCGGTCTGTTCTACGCCGATGTCCGTGCGGACG10.6802721088435374No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTAGTCGCCGT10.6802721088435374No Hit
GGCCGGACCAGTGCACGAGGAGAGGCGTACGGCCAGCCAGGCCCAAGTTT10.6802721088435374No Hit
CATAGCATAGTTTTTAATCGAAGGGGGGACGCAATAGGATAGGCAAAGCG10.6802721088435374No Hit
GTTCTATTCTTCCAAGAGAAGCGTGTAGCAGCACATACACGGGTCCTGTC10.6802721088435374No Hit
CCTATCCTCTACAAGCTCTGCCGACTATCAAGATCATGCTACAGTAAAGC10.6802721088435374No Hit
CTTATGGCGAGGCCGTCTACAGGAGCGGTTAAGCAGCCCAATCAATCCGA10.6802721088435374No Hit
ACACACCTTTAAGGCTGACGACGAGACATGGTGTTTAATTCTCAGTATTA10.6802721088435374No Hit
GATCTGGGCGGGGACGTAATCAACGCGAGCTGATGACCCCCGCTTACTAG10.6802721088435374No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTATATCACGGGGGTGGCCGG10.6802721088435374No Hit
GTGAAACGCGGCCGGAGCAGGCGATAACCATGTTACCGACCGAGCGGTCG10.6802721088435374No Hit
GGATATAAGGTCCGTGATCAAAAGGGAGACAGTCCAGACCACCAGCTACG10.6802721088435374No Hit
CTTATACACATCTGACGCTGACGACGATATGCAGTGTGTGGAACTTACTG10.6802721088435374No Hit
CTTTCCTAACCCCACTACATTGATGTTGCAGCAGTTGGCAAGTCTGTTTG10.6802721088435374No Hit
CTTCTCTTCCGACCGCCCAAGAGTGTTGCATACTCCCGTGGTGTGACGGG10.6802721088435374No Hit
CTTCCGATCTACATAGGGGCGAAGCCACGAATCGAAGCCCCAGTAAACGG10.6802721088435374No Hit
ATTCACCACCAGATGGGTGGCATCCCGACCAACATCCACGGTCAGGTGGT10.6802721088435374No Hit
GTGAAAACATAGTACCTGTGAAGATACAGGTTACCCGGGACAGATCCTGT10.6802721088435374No Hit
TAGACAACTCACCCTGCCGACTAACAGAAGGTAAGCCCCAGCCAGACAGT10.6802721088435374No Hit
AACCGGAGCTAGGTCTGCATGCGATAAAACTAGCATGCCACTTTACGCTT10.6802721088435374No Hit
TGATTTGAGACTTCAACAGTCATACTTTTTAACTCATCTATAAACTCTTT10.6802721088435374No Hit
CTTCCTACGCTCCTCTCTAATACTGACCAGGCTACCAGCCACTCGTGACC10.6802721088435374No Hit
CATCTGACGCTGCCGACGAATAGAGAAGTGTAGATCTCGGGGGTCGCCGT10.6802721088435374Illumina Single End PCR Primer 1 (95% over 24bp)
GGAGAAGTGGAGCAACTTGTGGCTCAGATATACATGGTAACTCCGTGACC10.6802721088435374No Hit
TTACACACCGGCCCCCGCCGTCCAACATCATCTTCTACCTGTCTGTTGTC10.6802721088435374No Hit
CATCTGACGCTGCCGACGAAAAGCGTGGTGTAGATCTCGGTGGTCGCCGT10.6802721088435374Illumina RNA PCR Primer (95% over 24bp)
CTTATCCACATCTGACGCTGCCGACGAATAGAGGGGTGTTGTTCTCGGTG10.6802721088435374No Hit
CCTTTGGCGCGAGATTGGAATAGACGGGCGCCTGTTCATCGAGGGAACGG10.6802721088435374No Hit
GTCCCCCGTTGAGACAGTGCCCAAATCGTTACGCGTTTCGTGCTGGTCGG10.6802721088435374No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCCT10.6802721088435374Illumina RNA PCR Primer (95% over 23bp)
AGGAGGAGGCTTGAAGCGCCAAGTGAGGAGACGGGACGTGGTGACACGCC10.6802721088435374No Hit
GTTCTCTTCCGACCTCCCACGGGGTGGTCTTCCTGTCTCTTATACACATC10.6802721088435374No Hit
ACTTTGGAGCGGTGAAGCAACAACAAACTGTCACAAAATGCACAAAGAAA10.6802721088435374No Hit
CCCTATTACCTAGAGAAAATTCAGCAGCAGTAGGGATAGAGGTTTCTCAA10.6802721088435374No Hit
CCCAATCTCAAAGCACCCATATCCAAATGCGACTCACCGAATACCGTTCC10.6802721088435374No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTCTGTCTCGTAAACA10.6802721088435374No Hit
CATCCGACGCTAAAGGCGGGAAAGACAGTCGAAAGATAGATCGGCGTCGC10.6802721088435374No Hit
CACCGGATCACTAAGTCTTTTTCGACCGTGCTCGACTTGTAAGTCTCCCA10.6802721088435374No Hit
GTACAGGGCTATCACACAATATGGCCGCACTTTCCAGAGCGTTCCGCTAA10.6802721088435374No Hit
GTCTTTCGCTATTTGAAAAATGCGGTTTAATATCTGTCGCTTATACACAT10.6802721088435374No Hit
CATCTGACGCTGCCGACGAAAAGAGAGGTGTAGATCTCGGTGGTCGCGGT10.6802721088435374Illumina RNA PCR Primer (95% over 23bp)
TGAGAATCGTTTGAATGAAGAAAAATACGAACTAGATAAAACTAAAACGG10.6802721088435374No Hit
AAGCACTGCAGTGACACCCCACGTATACGGCGGCGGTAACTATAGCTGTC10.6802721088435374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATATTTACG10.6802721088435374No Hit
ACTAAGATGTTTCAGCTCTCCGGGTCAACAATCTGACATGCCTGTCTCTT10.6802721088435374No Hit
ACTCAATATCAAGCTAAAGTAAAGCTCCATGGGGTCTTTCCGTCCCTGTC10.6802721088435374No Hit
CAGCGGAGGCTGCCACCCAGAAGACATGGCGGACAGTGCCAATGCCTGGC10.6802721088435374No Hit
CGGTCAACTACGTGCCAGCAGCCGCAGAAATACGCTGTCTCTTATACACA10.6802721088435374No Hit
ATCTGCCGCTACCATCTAATATAGGGGTGGGCATCTCGGTGGGGTCCTTA10.6802721088435374No Hit
GGAGAGCTGCAAGTAAGCTGTGAACCAGAGATTTCCGAATGGGGGAACCC10.6802721088435374No Hit
CCCAAACGGTCTTCTGCTCCAGTGTATTCCGGCCTTCTGATCGGTTCCTG10.6802721088435374No Hit
CTACAATGGACAATACAAAAGGCAGCGAAACCGCGAGGTCAAGCAAATCC10.6802721088435374No Hit
CCGACAAGACGCCGGCGCCCCTTCGGCCGCCTTCTTCAGCGGCACGCCCG10.6802721088435374No Hit
CTAGAATATAGGTTCCCGTACAGCAAACCGATACAGGTAGTCAAGATGAG10.6802721088435374No Hit
TGCACTCTACACCATCGATCTCGCCAACACCCAGGTTCTCCATATTGGCA10.6802721088435374No Hit
CATCCGACGCCCCCCTTGAATAGAGAGGTTAAGAACTCTGGAGACACTGT10.6802721088435374No Hit
AATATGCATTTTCCACTAATTTTTGAACACTCTTTTAAACTTAGCAACCT10.6802721088435374No Hit
TAGTTACCCCTTTCGTGCAAGACAGAACTTACCCGACAGGGAATTTCGCT10.6802721088435374No Hit
GTAAGATTCAGGAACGTGTCCTAGAGCCAGGGCTGCTGATCAACGTCCAC10.6802721088435374No Hit
TCCCATATCTCATGCGTTACAAAAACTCGTAGTTTGACGGGCGGTGTGTA10.6802721088435374No Hit
CACATCTGACGCTCCAGAAAAAGAGAGATGTGGAACACCGTGGTCTCGCC10.6802721088435374No Hit
GCCCAGCGGGACCAGCGCGAGAAGGAGTTTGTGACCTGCTGGGAGAAGCA10.6802721088435374No Hit
CATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGCGGACGCCCT10.6802721088435374No Hit
TCACGAAAGAGCACAAACCTCGTGCCAAGCCCGGTGAAAGGCCCGTCTCG10.6802721088435374No Hit
GTTCTAAGGGCGTTGAAGCATGACCGTAAGGACTGTCTCTTATCCCCATC10.6802721088435374No Hit
AACTGGCAGGGGCTCCCCGGGCGGTCAGGAGGAAGGGTAGACCGGTCTAC10.6802721088435374No Hit
CATCTGACGCTGCCGACGAATAGAAAAGTGTAGATCTCGGTGGTCGCCGT10.6802721088435374Illumina Single End PCR Primer 1 (96% over 27bp)
CTTCCAAACAAAGCGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAACGAT10.6802721088435374No Hit
CTTCTACTACTGCAAACCTACAGAGGAATTCAAAATTAGAATTCTAAGGT10.6802721088435374No Hit
CGTGAGAGTACTGCCCGGGAGACACACGGCCGGTGCCAACGTCATGTCTC10.6802721088435374No Hit
GTGCTCTTCCGATCTCGGCCGGGGACGTGTGCTCCTGTCTCTTATACACA10.6802721088435374No Hit
TAACGGTCCTAAGGTACCAAAATTCCTTGTCGGGTAAGTTCCGACCCGCA10.6802721088435374No Hit
CTTATACACATCTGACGCCGCCGACCATACTCCCTGTGTAAATCCAGGTG10.6802721088435374No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCCCGGTGGTGCACGA10.6802721088435374No Hit
ATATTCTTGTATTTCCTTGACCTAACGGTTTCGCTGCCCTTTGTATTGTC10.6802721088435374No Hit
CTTTAGCGGTATCTAGCACTTAATTTACTACATTTATGTAAACCTGACAA10.6802721088435374No Hit
CTGTACAGCTCAAGGAGAAAAAAAAGTAGAATTGGAGGAAGCAGCCAGAG10.6802721088435374No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTTGATATTGGTGGTC10.6802721088435374No Hit
TTACACTACAGATCGGAACGACGAACGGTTTAGATCCTGTCTCTTATACA10.6802721088435374No Hit
CATCTGACGCTATCGACGAAGCGAGCGGTGACCCGAAACCAGGTGACCTA10.6802721088435374No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCCCCGTG10.6802721088435374No Hit
CGTAGAACCCAGCTCAGAATATACACGATAAGGCGACGTCCTCACACCCG10.6802721088435374No Hit
TTTTTTTATTGGGTTTGTTGATTTTTCAAACGCACCCGTGCCAGATCGGA10.6802721088435374No Hit
ACCACAACCTGCTAATTTTTTTGTATTTTTAATAGCGACGGAGTTTCTCC10.6802721088435374No Hit
GGACTTTAATCGCTGAACAAACGAACCTTTAATATCGGCTGCACCATCGG10.6802721088435374No Hit
CTTATACACATCTGACGCTGCCGACGAATATAGAGGTGTACACCACGGTG10.6802721088435374No Hit
GGGTAACCTGCATCTTCACAGGTACTATGATTTCACCGAGTCTCTCGTTG10.6802721088435374No Hit
ATACACATCTGTCGCTGCCGATGAATAGAGAGGAGTCGCTTCCCGGGGAC10.6802721088435374No Hit
GAAGAGATGAGAAGAGATGAGAAGAGATGAGAAGAGATGAGAAGAGATGA10.6802721088435374No Hit
CTTCCGATCCCACCCGCGACGCGCAAGGGGGCCATCCGGGTCGACCTGGA10.6802721088435374No Hit
GTTAAACCCCACGAACGCTCTTCAGAAGCGATCAACCACCTACGAGCCCT10.6802721088435374No Hit
GTGAAGCACAGCCCCTCTAGACGTCGAGCTAGGGGCCCTTTAAGCCCCTC10.6802721088435374No Hit
GCACAGGGCTCCCCGCGAAAAAGAGAGGAGGATACGGCTGTAGGCTCAGG10.6802721088435374No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGC10.6802721088435374Illumina RNA PCR Primer (95% over 24bp)
GATATTAACAAGATTACACAAACATTTCGGTTAAGGAAAAGAACTCGTTT10.6802721088435374No Hit
GGGCTATCCCGAAATAGATCACGTCCTGGAAGCTGCCGTCGTACAAGCTC10.6802721088435374No Hit
CATTTGACGCTGCGGACGAATAAAAAGTTGTTAATCACGGTTGTTTTTGC10.6802721088435374No Hit
CATATACACATCCGACCCTTCCGGCGAGTGTAGATGTGTGGGGTCCCGGG10.6802721088435374No Hit
CATAATAACTTCTCGAATCGCATGGCCTTGTGCTGGCGATGCTTCATTCC10.6802721088435374No Hit
GGGCAGCGAAACCGCGAGGTCAAGCAAATCCAATAAAGTTGTTCTCCTGT10.6802721088435374No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGGC10.6802721088435374No Hit
CATCTGCCGCTGCCGACAATACACCGGGTGTAGATCCGGGTGGTGGCCGT10.6802721088435374No Hit
ATCATATACCTCTCACGCTCTAGCCGTAAACGATGCTAGCTAGCACTTGG10.6802721088435374No Hit
GTCAAGCAAATCCGTAAAGTTGGTCTAAGTTCGGATTGTAGTCTGCAGCT10.6802721088435374No Hit
CATTTAGACAGTGCCACAATCGTTAAGCCTTTCGTGCGGGTCGGGGCCTA10.6802721088435374No Hit
TCCCAGAGTAGAGATGCCAGACCATCATGGGAGTGGTTAGGGTTTGGGGT10.6802721088435374No Hit
CTTATACACATCTGACGCTGCCGACGATCCTCCTTGTTTAGATCTCGGTG10.6802721088435374No Hit
TTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATGTAGGGGG10.6802721088435374No Hit
GAACATGGGGGGACCATCATCAAAGGCTAAATACCTGTCTCTTATACACA10.6802721088435374No Hit
CTCCACAACCCGACAACCAATTCACCGCGGCATTCTGATCCCCGATTAGC10.6802721088435374No Hit
CATCTGACGCTGCCGACCAACCGACAGGTATCGTTGTCGATGGTTGCCTT10.6802721088435374No Hit
GAAAAGGGAGACGTGTGCTCTTCTCACATAAAAAGAGGGACCGGTGCCCT10.6802721088435374No Hit
GCCCCTAGCTAGCAAAGATATAAATCAAATTGTCAAGGCTTGATCCTGCA10.6802721088435374No Hit
CGATACAACGACATAACCCGTGTTAGGTTCCCCCATTCGGAAATCTCTGG10.6802721088435374No Hit
TATGTATACATCTGCCCTGCAGGTGTGCTACACCCATTAACCAGGCACCT10.6802721088435374No Hit
CAAGGTGCCCAAGGCCGAACAGGCCGAGCGTGTGAAGAAGGCGCTGGACA10.6802721088435374No Hit
CTCCGCCGCCGCCACCGCCCTGAAGGGGGTGGGCCTCGAGGCCGATGTGC10.6802721088435374No Hit
CATCACATCCTACCCGGTAGACGCAACGACCACATTCCCGCTCCGGCGGG10.6802721088435374No Hit
GACCACCAGCTAAGGTCCCAAAATATATGTTAAGTGGAAAAGGATGTGGC10.6802721088435374No Hit
CTTCAGACGCTCACCACGAAAAACACAAGACGCGATAGGGTATCAATGAG10.6802721088435374No Hit
CTTATACCCATCTGACGCTGCCGAAGAAGAAGATAGTGTAAACATCGGTG10.6802721088435374No Hit
CAAAAAAAATGCACAAGACGAACAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6802721088435374No Hit
CCACGGTTGTGACGTGGGCTTCCACATCAGACTTAAAAAACCGCCTACGC10.6802721088435374No Hit
GGCAGCGATCCCCGCCGAACATAAGCATATCAATAAGCGGAGGAAAAGAA10.6802721088435374No Hit
GTCCAAGCCATGATTCTGGATGAACTTATGTGTCTCCTGGCCGTGGACCT10.6802721088435374No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers