FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s94.351000000d86a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s94.351000000d86a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA593.3656588705077013No Hit
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC211.1979463776383343RNA PCR Primer, Index 12 (96% over 26bp)
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA181.026811180832858Illumina PCR Primer Index 5 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGC181.026811180832858Illumina PCR Primer Index 10 (96% over 26bp)
GAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA130.7415858528237307Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC120.6845407872219054RNA PCR Primer, Index 41 (96% over 26bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.6274957216200799No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.4563605248146035No Hit
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA60.3422703936109527RNA PCR Primer, Index 17 (96% over 28bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.3422703936109527No Hit
ATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTT50.2852253280091272Illumina PCR Primer Index 12 (95% over 22bp)
TCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG50.2852253280091272TruSeq Adapter, Index 12 (96% over 29bp)
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA40.22818026240730174TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT40.22818026240730174Illumina PCR Primer Index 7 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGACGCGTAGTAATCTCGTATGCCGTCT40.22818026240730174TruSeq Adapter, Index 12 (95% over 21bp)
TCTCCTAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG40.22818026240730174TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCAACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG40.22818026240730174TruSeq Adapter, Index 10 (96% over 29bp)
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA40.22818026240730174TruSeq Adapter, Index 12 (96% over 29bp)
GAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAA40.22818026240730174Illumina PCR Primer Index 10 (96% over 29bp)
ACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT40.22818026240730174Illumina PCR Primer Index 12 (95% over 24bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG30.17113519680547634No Hit
GCACCCCGCCTGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTA30.17113519680547634No Hit
ATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT30.17113519680547634TruSeq Adapter, Index 12 (95% over 22bp)
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC30.17113519680547634No Hit
TCTACGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG30.17113519680547634TruSeq Adapter, Index 3 (96% over 28bp)
AAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT30.17113519680547634TruSeq Adapter, Index 12 (95% over 24bp)
ATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT30.17113519680547634RNA PCR Primer, Index 12 (96% over 27bp)
GATCGGAAGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC20.11409013120365087Illumina PCR Primer Index 3 (96% over 25bp)
CCCCTACATAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC20.11409013120365087No Hit
GCACCCCTGTTGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTA20.11409013120365087No Hit
CCCCAGTTGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC20.11409013120365087No Hit
CCCCTGGTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC20.11409013120365087No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT20.11409013120365087No Hit
TCTCCAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA20.11409013120365087RNA PCR Primer, Index 41 (96% over 29bp)
GCACCCCGGTTAAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTA20.11409013120365087No Hit
AGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG20.11409013120365087Illumina PCR Primer Index 5 (95% over 24bp)
GCACCCCTGGGGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA20.11409013120365087No Hit
AGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.11409013120365087Illumina PCR Primer Index 10 (96% over 29bp)
CTCCGAGCCCACGATACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGA20.11409013120365087RNA PCR Primer, Index 10 (96% over 29bp)
GTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTTTTGTGGTTTC20.11409013120365087No Hit
AAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC20.11409013120365087No Hit
CGTCTCCCAGGTTAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGT20.11409013120365087No Hit
AGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.11409013120365087RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACGATACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG20.11409013120365087TruSeq Adapter, Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGCGCGTAGTAATCTCGTATGCCGTCTTC20.11409013120365087Illumina PCR Primer Index 12 (95% over 23bp)
TCTCCGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG20.11409013120365087Illumina PCR Primer Index 3 (96% over 28bp)
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA20.11409013120365087RNA PCR Primer, Index 12 (96% over 29bp)
CCCCTGGGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC20.11409013120365087No Hit
CGCATCGTGCACGCATTCGTTACCCGGCCCGTCTACGCGGAACACGCCAC20.11409013120365087No Hit
AAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC20.11409013120365087No Hit
GAGCCCACGAGACGCGTAGTAACCTCGTATGCCGTCTTCTGCTTGAAAAA20.11409013120365087Illumina PCR Primer Index 12 (96% over 27bp)
CACCCCTGCTCAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTAT20.11409013120365087No Hit
GCACCCCGGTTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA20.11409013120365087No Hit
GGCTATCGCAATTTCGCGACCAAGCTGTGGAACGCGTCGCGCTTCGCCGA20.11409013120365087No Hit
CCCCTGCTCAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC20.11409013120365087No Hit
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA20.11409013120365087TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAACGCGTAGTAATCTCGTATGCCGTC20.11409013120365087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA20.11409013120365087No Hit
ATACACATCTCCGAGCCCACGAGAGGCGTAGTAATCTCGTATGCCGTCTT20.11409013120365087Illumina PCR Primer Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT20.11409013120365087No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG20.11409013120365087TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT20.11409013120365087TruSeq Adapter, Index 12 (95% over 22bp)
GCACCCCGTAGTAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTA20.11409013120365087No Hit
CATCTCCGAGCCCACTAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT20.11409013120365087TruSeq Adapter, Index 12 (96% over 27bp)
AAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT20.11409013120365087RNA PCR Primer, Index 41 (95% over 24bp)
GATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11409013120365087No Hit
TCTCAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG20.11409013120365087Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACAGACGCGTAGTAATCTCGTATGCCGTC20.11409013120365087No Hit
AAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT20.11409013120365087No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)