FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s93.351000000d867d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s93.351000000d867d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3916
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG1794.570990806945863TruSeq Adapter, Index 7 (96% over 28bp)
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA962.4514811031664965RNA PCR Primer, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG571.4555669050051072RNA PCR Primer, Index 29 (96% over 30bp)
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA561.4300306435137897Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA471.2002042900919305Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAA471.2002042900919305TruSeq Adapter, Index 7 (96% over 28bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC411.0469867211440245RNA PCR Primer, Index 29 (96% over 27bp)
GAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAA380.9703779366700716RNA PCR Primer, Index 29 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.8682328907048007No Hit
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA320.8171603677221655RNA PCR Primer, Index 42 (96% over 28bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA250.6384065372829418RNA PCR Primer, Index 29 (96% over 30bp)
TCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG130.3319713993871297Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC130.3319713993871297Illumina Paired End PCR Primer 2 (96% over 26bp)
TCTCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGCTTG130.3319713993871297Illumina PCR Primer Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT120.30643513789581206No Hit
CCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAA120.30643513789581206RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGAGACTAGCGCTCAGCTCGTATGCCGTCTTCTGCTTG120.30643513789581206Illumina PCR Primer Index 7 (96% over 26bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG110.2808988764044944TruSeq Adapter, Index 10 (96% over 29bp)
TCTCCGAGCCCACGAGACTAGCGCTCAACTCGTATGCCGTCTTCTGCTTG110.2808988764044944Illumina PCR Primer Index 7 (96% over 26bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTGCAGCT110.2808988764044944No Hit
TCTCCGAGCCCACGAGACTAGCGCTCACCTCGTATGCCGTCTTCTGCTTG110.2808988764044944Illumina PCR Primer Index 7 (96% over 26bp)
ATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT100.2553626149131767Illumina PCR Primer Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT90.22982635342185903No Hit
CTTATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT90.22982635342185903No Hit
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT90.22982635342185903Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT90.22982635342185903TruSeq Adapter, Index 7 (95% over 21bp)
CCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAA90.22982635342185903RNA PCR Primer, Index 7 (96% over 28bp)
TCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA90.22982635342185903Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT90.22982635342185903No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT80.20429009193054137Illumina PCR Primer Index 7 (95% over 22bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA80.20429009193054137RNA PCR Primer, Index 29 (96% over 30bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTAGCGCT80.20429009193054137No Hit
TCTCCGAGCCAACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG80.20429009193054137Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCGAGCCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT80.20429009193054137RNA PCR Primer, Index 29 (96% over 29bp)
TCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG70.17875383043922372TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT70.17875383043922372TruSeq Adapter, Index 10 (95% over 22bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.17875383043922372TruSeq Adapter, Index 10 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTT70.17875383043922372Illumina PCR Primer Index 7 (95% over 21bp)
ACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT70.17875383043922372RNA PCR Primer, Index 29 (95% over 22bp)
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.17875383043922372Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGT70.17875383043922372No Hit
TATCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.15321756894790603RNA PCR Primer, Index 29 (96% over 30bp)
AGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA60.15321756894790603TruSeq Adapter, Index 7 (96% over 28bp)
TCCGAGCCCACGAGACTAGCGCTCACCTCGTATGCCGTCTTCTGCTTGAA60.15321756894790603Illumina PCR Primer Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT60.15321756894790603No Hit
ACACATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT60.15321756894790603RNA PCR Primer, Index 10 (95% over 24bp)
AGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAA60.15321756894790603Illumina Paired End PCR Primer 2 (96% over 29bp)
CTCCGAGCCCACGAGACTAGCGCTCACCTCGTATGCCGTCTTCTGCTTGA60.15321756894790603Illumina PCR Primer Index 7 (96% over 26bp)
CTCCGAGCCCACGAGACTAGCGCTCAGCTCGTATGCCGTCTTCTGCTTGA50.12768130745658834Illumina PCR Primer Index 7 (96% over 26bp)
CATCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT50.12768130745658834RNA PCR Primer, Index 29 (96% over 29bp)
TCTCCTAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG50.12768130745658834TruSeq Adapter, Index 7 (96% over 28bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG50.12768130745658834RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT50.12768130745658834No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG50.12768130745658834No Hit
CGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA50.12768130745658834RNA PCR Primer, Index 29 (96% over 30bp)
CATCTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT50.12768130745658834RNA PCR Primer, Index 29 (96% over 28bp)
CATCTCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT50.12768130745658834TruSeq Adapter, Index 7 (96% over 27bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA50.12768130745658834Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCACACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG50.12768130745658834Illumina PCR Primer Index 7 (96% over 28bp)
ATACACATATCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT50.12768130745658834RNA PCR Primer, Index 7 (95% over 21bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTGAGC50.12768130745658834No Hit
TCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG50.12768130745658834TruSeq Adapter, Index 10 (96% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.12768130745658834No Hit
TCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGCTTGAA50.12768130745658834RNA PCR Primer, Index 29 (96% over 27bp)
CTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA50.12768130745658834RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGT40.10214504596527069No Hit
TCTCCGAGCCCACGGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA40.10214504596527069RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCTAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG40.10214504596527069Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG40.10214504596527069Illumina PCR Primer Index 7 (96% over 28bp)
TTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA40.10214504596527069RNA PCR Primer, Index 7 (96% over 28bp)
AAAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC40.10214504596527069No Hit
TCTCCGAGCCCACGAACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGA40.10214504596527069Illumina Paired End PCR Primer 2 (96% over 29bp)
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA40.10214504596527069Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT40.10214504596527069No Hit
TCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10214504596527069RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT40.10214504596527069Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA40.10214504596527069RNA PCR Primer, Index 7 (96% over 28bp)
TCTACGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG40.10214504596527069TruSeq Adapter, Index 5 (96% over 28bp)
TCTCCGAGCCCACGAGACTGCAGCTAAACTCGTATGCCGTCTTCTGCTTG40.10214504596527069Illumina PCR Primer Index 12 (96% over 26bp)
CATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT40.10214504596527069TruSeq Adapter, Index 10 (96% over 27bp)
TCCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10214504596527069Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGACCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG40.10214504596527069TruSeq Adapter, Index 10 (96% over 29bp)
CATCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCT40.10214504596527069TruSeq Adapter, Index 10 (96% over 27bp)
TTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10214504596527069RNA PCR Primer, Index 29 (96% over 30bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA40.10214504596527069No Hit
TCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT40.10214504596527069Illumina PCR Primer Index 10 (96% over 28bp)
TCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG40.10214504596527069TruSeq Adapter, Index 7 (96% over 28bp)
CTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA40.10214504596527069RNA PCR Primer, Index 29 (96% over 27bp)
CTCCGAGCCCACGAGACTAGCGCTCATATCGTATGCCGTCTTCTGCTTGA40.10214504596527069Illumina PCR Primer Index 7 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA301.1282409E-679.166671
TTCCGAT352.8214145E-667.857152
TCCGATC352.8214145E-667.857153
CCGATCT352.8214145E-667.857154
ATCTTTT301.14606984E-463.3333367
GATCTTT301.14606984E-463.3333366
TCTTTTT301.14606984E-463.3333368
CGATCTT406.2345243E-659.3749965
CTTATAC556.270602E-751.8181841
TTATACA601.1433749E-647.52
CTTTTTT404.7509468E-447.4999969
TATACAC651.9855597E-643.8461533
TCTCCGA1109.131327E-1038.8636361
ACACATC851.2556267E-533.5294156
TACACAT901.8577428E-531.6666665
ATACACA901.8577428E-531.6666664
CACATCT752.5556915E-431.6666647
CTCCGAG1409.6843E-930.5357152
ACATCTC855.3263165E-427.9411778
TCCGAGC1552.613524E-827.5806453
Produced by FastQC (version 0.11.2)