FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s92.351000000d8647.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s92.351000000d8647.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15255234
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT2611071.7115896091793807No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT1834421.2024856518097329No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG1403160.9197892343047638Illumina PCR Primer Index 7 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT1107200.7257836884049107Illumina PCR Primer Index 7 (95% over 22bp)
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT552050.36187579947970644Illumina PCR Primer Index 7 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC437570.28683270279564377RNA PCR Primer, Index 41 (95% over 22bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA430780.2823817713972791No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA375260.24598770494113692Illumina PCR Primer Index 7 (96% over 27bp)
ATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAGTTGTT361830.23718416905306075No Hit
CTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA331040.2170009322701966No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG279690.18334035387461117RNA PCR Primer, Index 41 (96% over 27bp)
GTGTGTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGAT272330.17851578022336467No Hit
ACAATGGACAATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCA265430.17399274242532103No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT259140.16986956738913345No Hit
GTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGT251400.16479589890263235No Hit
GTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACT242640.15905360743729005No Hit
CCCTTAAGCTTAGTTGCCATCATTCAGTTGGGCACTCTAAGTTGACTGCC234670.1538291710241875No Hit
CCCTGGGAAGGGTGGCCAGAGCAGGTGATAGCCCTGTAACCGACCGAGCG225560.14785745010532123No Hit
GATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAGTTGCAGACTA218270.1430787623447795No Hit
GTGTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTA214640.14069925115537396No Hit
GTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATGATTTG212950.13959143465121546No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAG212820.1395062179970494No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA210590.13804442462173966No Hit
GTGTAAAGGCAGAAGGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGG192150.1259567699846492No Hit
ATTCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGGCC187170.12269231661736556No Hit
GGACAATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAG183560.12032591568244709No Hit
CTACAATCCGAACTGAGAACAACTTTATGGGATTTGCTTGACCTCGCGGT181750.11913943765136609No Hit
GTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGC173480.11371834742095731No Hit
ACTTTATGGGATTTGCTTGACCTCGCGGTTTCGCTGCCCTTTGTATTGTC164510.10783839828350059No Hit
GTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCG162950.10681579843350812No Hit
CCTTATGATTTGGGCTACACACGTGCTACAATGGACAATACAAAGGGCAG162300.10638971516267794No Hit
GTAGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAG155970.10224031961751619No Hit
ATCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTG155410.10187323249187788No Hit
GTATTGTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATG154310.10115216849508832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT645850.066.1114652
CGATCTA124350.064.594415
TCCGATC683400.062.3329623
CCGATCT684000.062.1046684
CGATCTT146150.055.024395
CGATCTC202250.051.7630845
GCTCTTC240000.050.2712363
CGATCTG328750.049.154775
GATCTAG54600.048.9793326
TCTTCCG247800.048.6121755
ATCTGGC37400.047.7522057
GATCTGC89200.046.8613626
GATCTAT31050.046.5060126
CTCTTCC262800.045.765214
GATCTTC45050.044.6008036
ATCTGGT32800.044.457167
ATCTGCG25250.044.394367
GATCTGA78600.043.572236
CTTCCGA986300.043.439851
GATCTTT73150.043.1821676
Produced by FastQC (version 0.11.2)