FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s91.351000000d862d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s91.351000000d862d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA1172.9930928626247124Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG631.6116653875671527RNA PCR Primer, Index 29 (96% over 30bp)
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA541.3814274750575595Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA521.3302634944998721Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA511.3046815042210285TruSeq Adapter, Index 11 (96% over 30bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA320.8186236889229983RNA PCR Primer, Index 29 (96% over 30bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA300.7674597083653107RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG250.6395497569710923Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG230.588385776413405TruSeq Adapter, Index 11 (96% over 30bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA230.588385776413405RNA PCR Primer, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC220.5628037861345613RNA PCR Primer, Index 29 (96% over 27bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA190.4860578152980302RNA PCR Primer, Index 40 (96% over 29bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA180.4604758250191865TruSeq Adapter, Index 11 (96% over 30bp)
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG180.4604758250191865TruSeq Adapter, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC170.4348938347403428RNA PCR Primer, Index 11 (96% over 27bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA170.4348938347403428RNA PCR Primer, Index 29 (96% over 30bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA170.4348938347403428No Hit
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA160.40931184446149915RNA PCR Primer, Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGT150.38372985418265537No Hit
TCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG140.3581478639038117Illumina PCR Primer Index 11 (96% over 30bp)
TCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG130.33256587362496803TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG130.33256587362496803TruSeq Adapter, Index 11 (96% over 30bp)
CCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAA130.33256587362496803RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT120.3069838833461243No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC120.3069838833461243RNA PCR Primer, Index 41 (96% over 26bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTGCAGCT120.3069838833461243No Hit
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT110.28140189306728064No Hit
TCTCCGAGCCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT100.25581990278843697Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT100.25581990278843697TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTT100.25581990278843697Illumina PCR Primer Index 11 (95% over 23bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC100.25581990278843697RNA PCR Primer, Index 6 (96% over 25bp)
ATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT100.25581990278843697TruSeq Adapter, Index 11 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.25581990278843697No Hit
CATCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT90.23023791250959325RNA PCR Primer, Index 29 (96% over 29bp)
ACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT90.23023791250959325Illumina PCR Primer Index 11 (100% over 24bp)
CATCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT90.23023791250959325TruSeq Adapter, Index 11 (96% over 29bp)
TCTCCAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA90.23023791250959325RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGAGCCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT90.23023791250959325RNA PCR Primer, Index 29 (96% over 29bp)
CATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT90.23023791250959325TruSeq Adapter, Index 11 (96% over 28bp)
TCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA80.20465592223074958TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG80.20465592223074958Illumina PCR Primer Index 3 (96% over 28bp)
TCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG80.20465592223074958Illumina PCR Primer Index 10 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.20465592223074958No Hit
CTTATACACATCTCCGAGCCCACGAGAGGAGCTACATCTCGTATGCCGTC80.20465592223074958RNA PCR Primer, Index 11 (95% over 21bp)
TCTCCGAGCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA80.20465592223074958Illumina PCR Primer Index 11 (96% over 30bp)
TATCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.17907393195190585RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.17907393195190585TruSeq Adapter, Index 10 (96% over 29bp)
CATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT70.17907393195190585Illumina PCR Primer Index 11 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT70.17907393195190585TruSeq Adapter, Index 10 (95% over 22bp)
CTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA70.17907393195190585Illumina PCR Primer Index 11 (96% over 30bp)
CCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA70.17907393195190585Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAAGCTCGTATGCCGTCTTCTGCTTG70.17907393195190585Illumina PCR Primer Index 12 (96% over 26bp)
CTTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT60.15349194167306215No Hit
ATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT60.15349194167306215Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT60.15349194167306215Illumina PCR Primer Index 11 (100% over 24bp)
TCTCCGAGCCCACGAGAGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA60.15349194167306215Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA60.15349194167306215RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.15349194167306215RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.15349194167306215RNA PCR Primer, Index 29 (96% over 30bp)
TCCGAGCCCACGAGACGGAGCTACACCTCGTATGCCGTCTTCTGCTTGAA60.15349194167306215Illumina PCR Primer Index 11 (96% over 29bp)
TATCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.15349194167306215RNA PCR Primer, Index 11 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT60.15349194167306215Illumina PCR Primer Index 10 (95% over 22bp)
TCTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG60.15349194167306215TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTCTTC60.15349194167306215Illumina PCR Primer Index 11 (95% over 24bp)
GCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA60.15349194167306215Illumina PCR Primer Index 11 (100% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.15349194167306215No Hit
CATCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT60.15349194167306215TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCAACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG60.15349194167306215RNA PCR Primer, Index 40 (96% over 29bp)
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.15349194167306215Illumina PCR Primer Index 10 (96% over 29bp)
ACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCT60.15349194167306215Illumina PCR Primer Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT50.12790995139421849No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT50.12790995139421849No Hit
ACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT50.12790995139421849Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.12790995139421849TruSeq Adapter, Index 11 (96% over 30bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACCCTAAGA50.12790995139421849No Hit
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA50.12790995139421849Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTC50.12790995139421849Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT50.12790995139421849No Hit
ATTCGAGAGAGTGGGGATGAGACGGGATGTGCCCAACCGTCGTTGCTGCG50.12790995139421849No Hit
CATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT50.12790995139421849TruSeq Adapter, Index 10 (96% over 27bp)
TCTCCGAGCCCACCGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT50.12790995139421849Illumina PCR Primer Index 11 (100% over 28bp)
TCTCAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG50.12790995139421849RNA PCR Primer, Index 40 (96% over 29bp)
CTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA50.12790995139421849RNA PCR Primer, Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGT50.12790995139421849No Hit
CTTATACACATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGT40.10232796111537479No Hit
TCTCCGAGCCCACGAGGCTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG40.10232796111537479RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.10232796111537479Illumina PCR Primer Index 11 (96% over 30bp)
CATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT40.10232796111537479Illumina PCR Primer Index 10 (96% over 27bp)
ATACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTC40.10232796111537479Illumina PCR Primer Index 11 (95% over 24bp)
GAGCCCACGAGACGGAGCTACAACTCGTATGCCGTCTTCTGCTTGAAAAA40.10232796111537479Illumina PCR Primer Index 11 (96% over 29bp)
TCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10232796111537479RNA PCR Primer, Index 29 (96% over 30bp)
CATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT40.10232796111537479TruSeq Adapter, Index 11 (96% over 28bp)
AAAAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT40.10232796111537479No Hit
AGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA40.10232796111537479TruSeq Adapter, Index 6 (96% over 28bp)
CTCCGAGCCCACGAGACTGCAGCTAAACTCGTATGCCGTCTTCTGCTTGA40.10232796111537479Illumina PCR Primer Index 12 (96% over 26bp)
TCCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10232796111537479Illumina PCR Primer Index 10 (96% over 29bp)
CACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG40.10232796111537479TruSeq Adapter, Index 11 (96% over 26bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG40.10232796111537479TruSeq Adapter, Index 10 (96% over 29bp)
ACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCT40.10232796111537479Illumina PCR Primer Index 11 (96% over 25bp)
TCTCCGAGCCCACGAGGCGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.10232796111537479TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGATACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG40.10232796111537479Illumina PCR Primer Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGCTGCAGCTAATCTCGTATGCCGTC40.10232796111537479No Hit
TCTCCGAGCCCAGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA40.10232796111537479RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCACACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG40.10232796111537479RNA PCR Primer, Index 40 (96% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGCTTG40.10232796111537479Illumina PCR Primer Index 12 (96% over 26bp)
CGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA40.10232796111537479TruSeq Adapter, Index 11 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCCC200.001922045171.251
TATACAC451.2534385E-552.7777753
CTTATAC451.2534385E-552.7777751
TTATACA502.3390141E-547.5000042
TTCCGAT300.00957628147.52
CAGATCG150.009947847.510-11
AAAACCA150.009947847.562-63
CTTCCGA300.00957628147.51
CACATCT651.1011568E-436.5384647
TACACAT651.1011568E-436.5384642
ACACATC701.7035034E-433.9285743
ATACACA701.7035034E-433.9285741
TCTCCGA700.00742346327.1428591
AGATCGG500.001511491723.75000210-11
GAAGAGC550.002631760721.5909116-17
CGGAAGA550.002631760721.5909114-15
ATCGGAA600.00435977419.79166812-13
CGAGCCC1609.2720025E-417.81255
CGAGACG950.003327849315.010-11
CTACATC1000.004682548314.25000120-21
Produced by FastQC (version 0.11.2)