FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s90.351000000d8603.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s90.351000000d8603.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA491.573538856775851Illumina PCR Primer Index 5 (96% over 28bp)
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA481.5414258188824663Illumina PCR Primer Index 9 (100% over 27bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA280.8991650610147719Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG270.8670520231213872Illumina PCR Primer Index 11 (96% over 29bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA260.8349389852280027RNA PCR Primer, Index 40 (96% over 29bp)
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA240.7707129094412332Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA210.674373795761079Illumina PCR Primer Index 11 (96% over 29bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA180.5780346820809248Illumina PCR Primer Index 11 (100% over 29bp)
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA170.5459216441875401TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT140.44958253050738595No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG140.44958253050738595TruSeq Adapter, Index 12 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC130.41746949261400135RNA PCR Primer, Index 12 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.41746949261400135No Hit
TCTCCGAGCCAACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG130.41746949261400135RNA PCR Primer, Index 40 (96% over 29bp)
AGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA110.35324341682723187TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCTAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG110.35324341682723187RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCACGATACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG100.3211303789338471Illumina PCR Primer Index 12 (96% over 29bp)
ATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT90.2890173410404624TruSeq Adapter, Index 12 (95% over 22bp)
TCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG90.2890173410404624TruSeq Adapter, Index 9 (96% over 28bp)
TCTCCGAGCCCACTAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG90.2890173410404624TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG90.2890173410404624Illumina PCR Primer Index 9 (96% over 28bp)
GATCGGAAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGC80.2569043031470777Illumina PCR Primer Index 11 (96% over 26bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA80.2569043031470777TruSeq Adapter, Index 11 (96% over 30bp)
TCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG80.2569043031470777Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT80.2569043031470777No Hit
TCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAA80.2569043031470777TruSeq Adapter, Index 9 (96% over 28bp)
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA80.2569043031470777RNA PCR Primer, Index 12 (96% over 29bp)
CTTATACACATCTCAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT70.22479126525369297No Hit
CTTATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT70.22479126525369297No Hit
CTTATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGT70.22479126525369297No Hit
CTCCGAGCCCACGATACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA70.22479126525369297RNA PCR Primer, Index 11 (96% over 29bp)
CCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAA70.22479126525369297Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA70.22479126525369297RNA PCR Primer, Index 11 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT70.22479126525369297No Hit
CATCTCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT70.22479126525369297TruSeq Adapter, Index 12 (96% over 28bp)
TCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG70.22479126525369297TruSeq Adapter, Index 11 (96% over 29bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTACGCTG60.1926782273603083No Hit
TCTCCGAGCCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT60.1926782273603083Illumina PCR Primer Index 12 (96% over 28bp)
ACACATCTCAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT60.1926782273603083Illumina PCR Primer Index 11 (95% over 24bp)
TCTCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA60.1926782273603083TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCACACGAGACGCGTAGTAATCTCGTATGCCGT60.1926782273603083No Hit
ACACATCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCT60.1926782273603083RNA PCR Primer, Index 11 (95% over 24bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC60.1926782273603083RNA PCR Primer, Index 6 (96% over 25bp)
AGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA60.1926782273603083Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCCGAGCACACGAGACGCGTAGTAATCTCGTATGCCGTCTT60.1926782273603083Illumina PCR Primer Index 12 (95% over 22bp)
ATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTT60.1926782273603083Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGCATGCGCAGATCTCGTATGCCGTC50.16056518946692355No Hit
TCTCCTAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG50.16056518946692355Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT50.16056518946692355Illumina PCR Primer Index 12 (96% over 27bp)
TCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.16056518946692355Illumina PCR Primer Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC50.16056518946692355RNA PCR Primer, Index 11 (96% over 27bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.16056518946692355TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG50.16056518946692355RNA PCR Primer, Index 47 (96% over 29bp)
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA50.16056518946692355RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCACGATACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG50.16056518946692355Illumina PCR Primer Index 9 (96% over 28bp)
TCTCCTAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG50.16056518946692355TruSeq Adapter, Index 12 (96% over 29bp)
AGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA50.16056518946692355TruSeq Adapter, Index 6 (96% over 28bp)
GATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC50.16056518946692355RNA PCR Primer, Index 47 (96% over 26bp)
CTTCAAATCTCCAACGCCCGCGACGGATAGGGACCGAACTGTCTCACGAC50.16056518946692355No Hit
TCTCCGAGCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA50.16056518946692355Illumina PCR Primer Index 12 (96% over 29bp)
TCTCCGAGCCCACGAGACTACGCTGCACCTCGTATGCCGTCTTCTGCTTG50.16056518946692355TruSeq Adapter, Index 3 (96% over 26bp)
CATCTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT40.12845215157353884RNA PCR Primer, Index 47 (96% over 27bp)
CATCTCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTT40.12845215157353884Illumina PCR Primer Index 11 (96% over 28bp)
ACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT40.12845215157353884Illumina Paired End PCR Primer 2 (95% over 22bp)
TCTCCGAGCCCACGATACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG40.12845215157353884TruSeq Adapter, Index 11 (96% over 29bp)
CCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAA40.12845215157353884RNA PCR Primer, Index 12 (96% over 29bp)
TCTCCGAGCCCAGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA40.12845215157353884Illumina PCR Primer Index 5 (96% over 28bp)
ATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT40.12845215157353884TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTT40.12845215157353884TruSeq Adapter, Index 11 (95% over 22bp)
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.12845215157353884TruSeq Adapter, Index 11 (96% over 30bp)
CATCTCCTAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT40.12845215157353884TruSeq Adapter, Index 12 (96% over 27bp)
ACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT40.12845215157353884Illumina PCR Primer Index 12 (95% over 24bp)
TCTCCGAGCCCACGATACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG40.12845215157353884RNA PCR Primer, Index 40 (96% over 29bp)
ATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT40.12845215157353884TruSeq Adapter, Index 9 (95% over 21bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGCGTAGT40.12845215157353884No Hit
CATCTCAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCT40.12845215157353884Illumina PCR Primer Index 11 (96% over 27bp)
CTCCTAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA40.12845215157353884Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG40.12845215157353884RNA PCR Primer, Index 47 (96% over 29bp)
CTTATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGT40.12845215157353884No Hit
CTTATACACATCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT40.12845215157353884No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAG156.007537E-495.03
TCGAGCC156.007537E-495.05
CTCGAGC156.007537E-495.04
ATCTCGA200.001883789571.252
CATCTCC1003.5614343E-528.51
ATCTCCG959.721074E-425.02
TCTCCGA1302.1277888E-421.9230771
CTCCGAG1403.516585E-420.3571412
CGAGCCC1454.4591344E-419.6551725
CCGAGCC1505.607419E-419.04
TCCGAGC1556.996743E-418.3870963
GAGCCCA1700.001303361716.7647088
AGCCCAC1750.001583573616.2857159
CACGAGA1600.0078242714.8437499
CCACGAG1650.00983494914.3939398
TCTGCTT2001.2433848E-613.062544-45
GTCTTCT2001.2433848E-613.062540-41
TATGCCG2051.6430622E-612.74390334-35
CTTGAAA2051.6430622E-612.74390348-49
CTCGTAT2051.6430622E-612.74390330-31
Produced by FastQC (version 0.11.2)