FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s9.351000000d7c37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s9.351000000d7c37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4738190
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT966522.0398506602732267No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA604851.2765423083498129No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG565501.193493718065337TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT554111.1694550028597417TruSeq Adapter, Index 11 (95% over 23bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT548881.158417032664372No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA373670.7886344785667101No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT373510.7882967968781327RNA PCR Primer, Index 11 (96% over 25bp)
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT359210.7581164959615381No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT322370.6803652871666185TruSeq Adapter, Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC163840.34578604910313854TruSeq Adapter, Index 11 (95% over 21bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA143900.3037024686641946RNA PCR Primer, Index 11 (96% over 30bp)
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG131260.27702561526658914No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG129340.2729734350036617No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT120800.25494967487584924No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG119770.25277584900563294No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA110020.23219837110795474No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG109840.23181847920830528TruSeq Adapter, Index 11 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT104380.22029509158560545RNA PCR Primer, Index 11 (95% over 22bp)
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC103370.21816347592646138No Hit
GCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCGGCCCACAATAAC91050.19216198590601052No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC88360.18648471251680493No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA86580.18272800373138268No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC85410.18025870638366126No Hit
ACACAACACAACACAACACAACACAACACAACACAACACAACACAACACA75430.15919581105865319No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT75150.15860486810364297No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT73530.15518584100679794No Hit
ACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATA70430.1486432582906131No Hit
GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT69410.14649053752593288No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT67710.14290266958479927No Hit
GAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATA67660.14279714405711885No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA67410.14226951641871685No Hit
TCTTATATGTGATTAAATACTTGTATTTTTTCATATGTTCCTCCTATTTA67010.14142531219727364No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT66400.14013790075957275No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT62300.13148480748977984No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA61680.13017629094654287No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA61310.1293954020417079No Hit
CAATAACACTCGTTTAAGAGCACTAATGCAGGTTTTTAAATAGGAGGAAC60620.12793914975971837No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC60340.12734820680470813No Hit
AGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGA59780.12616632089468763No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA56480.11920163606778116No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT55100.1162891315038021No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC54010.11398867500036934No Hit
GGTTAGTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTT52090.10993649473744194No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA51670.10905008030492656No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA51600.10890234456617401No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA50790.1071928310177515No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG50160.10586320936897846No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA48400.10214871079462834No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT48340.10202208016141184No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA47720.10071356361817486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT140300.029.7597314
CTTCCGA142450.029.6693821
TCCGATC144350.029.0893023
TTCCGAT148250.028.3560922
ACGGTCC34900.028.0360419
GCTTGTC34250.026.7652688
ATCTTTT55450.026.3829927
CGACGGT37900.025.9421547
GACGGTC38700.025.5286168
GTCCTAG32350.025.2768751
ACGACGG41150.023.894526
ATGCTTG38000.023.8752296
CGATCTT72400.023.8158365
GTCTTAC48100.023.7211281
GTATAGT56550.023.6234261
TCTTACG43100.023.3644962
CTTACGA43050.023.1709563
CCTAGAT34700.022.7235623
TAGATAC38450.021.989815
GTACTAA54750.021.9499846
Produced by FastQC (version 0.11.2)