FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s89.351000000d85f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s89.351000000d85f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3887
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA2135.479804476459995Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC842.161049652688449Illumina PCR Primer Index 10 (96% over 25bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA802.058142526369951Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGCT441.1319783895034732No Hit
TCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG411.0547980447646Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG401.0290712631849754TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG370.9518909184461024Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG320.8232570105479805Illumina PCR Primer Index 10 (96% over 28bp)
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA320.8232570105479805Illumina PCR Primer Index 10 (96% over 28bp)
TCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG310.797530228968356Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG300.7718034473887316Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG300.7718034473887316TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG240.6174427579109854Illumina PCR Primer Index 11 (96% over 29bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA220.5659891947517366Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTT200.5145356315924877TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG190.48880885001286345TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT180.463082068433239No Hit
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA180.463082068433239Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT180.463082068433239Illumina PCR Primer Index 10 (96% over 26bp)
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT180.463082068433239TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT180.463082068433239RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA160.41162850527399025RNA PCR Primer, Index 29 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGT160.41162850527399025No Hit
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA160.41162850527399025Illumina PCR Primer Index 10 (96% over 28bp)
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA150.3859017236943658Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT150.3859017236943658Illumina PCR Primer Index 10 (96% over 26bp)
CATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT150.3859017236943658Illumina PCR Primer Index 10 (96% over 26bp)
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA150.3859017236943658Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT130.33444816053511706Illumina PCR Primer Index 10 (96% over 27bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA130.33444816053511706Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCT130.33444816053511706RNA PCR Primer, Index 10 (95% over 23bp)
ATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT120.3087213789554927Illumina PCR Primer Index 10 (95% over 21bp)
CTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA120.3087213789554927RNA PCR Primer, Index 10 (96% over 28bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA120.3087213789554927Illumina PCR Primer Index 11 (100% over 29bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTACGCTG110.2829945973758683No Hit
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA110.2829945973758683Illumina PCR Primer Index 10 (96% over 28bp)
TATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG110.2829945973758683Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA110.2829945973758683No Hit
ACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT100.25726781579624386Illumina PCR Primer Index 10 (95% over 23bp)
TCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.25726781579624386RNA PCR Primer, Index 29 (96% over 28bp)
CTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.25726781579624386Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.25726781579624386RNA PCR Primer, Index 10 (96% over 28bp)
CATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT90.2315410342166195Illumina PCR Primer Index 10 (96% over 26bp)
CTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.20581425263699513Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA80.20581425263699513TruSeq Adapter, Index 11 (96% over 30bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGC80.20581425263699513No Hit
TCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.20581425263699513RNA PCR Primer, Index 29 (96% over 28bp)
ATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTT80.20581425263699513TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG80.20581425263699513TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT80.20581425263699513Illumina PCR Primer Index 10 (95% over 23bp)
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA80.20581425263699513Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCATGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG70.18008747105737072TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT70.18008747105737072No Hit
CATCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCT70.18008747105737072TruSeq Adapter, Index 10 (96% over 26bp)
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA70.18008747105737072Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT70.18008747105737072Illumina PCR Primer Index 10 (95% over 21bp)
TCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.18008747105737072Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT70.18008747105737072TruSeq Adapter, Index 10 (96% over 26bp)
CATCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT70.18008747105737072RNA PCR Primer, Index 29 (96% over 27bp)
CACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG60.15436068947774634Illumina PCR Primer Index 10 (95% over 24bp)
CTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.15436068947774634Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC60.15436068947774634RNA PCR Primer, Index 11 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT60.15436068947774634No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.15436068947774634TruSeq Adapter, Index 11 (96% over 30bp)
AAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT60.15436068947774634Illumina PCR Primer Index 10 (95% over 23bp)
AGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA60.15436068947774634Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.15436068947774634Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTT60.15436068947774634RNA PCR Primer, Index 29 (96% over 27bp)
GAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAAAAA60.15436068947774634RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT60.15436068947774634No Hit
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA60.15436068947774634Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.15436068947774634Illumina PCR Primer Index 10 (96% over 28bp)
CCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAA60.15436068947774634Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA60.15436068947774634RNA PCR Primer, Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTC60.15436068947774634No Hit
TCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.15436068947774634Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT60.15436068947774634No Hit
CTTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT50.12863390789812193No Hit
CACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG50.12863390789812193TruSeq Adapter, Index 10 (95% over 24bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCCTGAAAAA50.12863390789812193RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.12863390789812193TruSeq Adapter, Index 11 (96% over 30bp)
CATCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.12863390789812193RNA PCR Primer, Index 29 (96% over 27bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA50.12863390789812193RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGAGCCCACTAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG50.12863390789812193Illumina PCR Primer Index 11 (96% over 29bp)
TCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG50.12863390789812193Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACGAGAACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.12863390789812193Illumina PCR Primer Index 10 (96% over 28bp)
ACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC50.12863390789812193RNA PCR Primer, Index 29 (96% over 25bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.12863390789812193No Hit
TCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG50.12863390789812193TruSeq Adapter, Index 11 (96% over 29bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.12863390789812193Illumina PCR Primer Index 11 (96% over 30bp)
CATCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT50.12863390789812193TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.12863390789812193Illumina PCR Primer Index 10 (96% over 27bp)
TCTCCGACCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.12863390789812193RNA PCR Primer, Index 29 (96% over 28bp)
TCTCCGAGCCCCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.12863390789812193RNA PCR Primer, Index 29 (96% over 28bp)
TCTCCGAGCCCACCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.12863390789812193Illumina PCR Primer Index 10 (96% over 27bp)
CTTATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGT40.10290712631849756No Hit
TCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10290712631849756RNA PCR Primer, Index 29 (96% over 28bp)
TCTCCGAACCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.10290712631849756TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG40.10290712631849756TruSeq Adapter, Index 11 (96% over 29bp)
TCTCCGAGCCCACGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA40.10290712631849756Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCATGAGACACTCGCTAATCTCGTATGCCGTCTT40.10290712631849756TruSeq Adapter, Index 10 (95% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCAACGAGACACTCGCT40.10290712631849756No Hit
CATCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT40.10290712631849756TruSeq Adapter, Index 11 (96% over 28bp)
TCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA40.10290712631849756TruSeq Adapter, Index 10 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10290712631849756No Hit
CTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.10290712631849756TruSeq Adapter, Index 10 (96% over 28bp)
GCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA40.10290712631849756Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGAAACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.10290712631849756TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT40.10290712631849756Illumina PCR Primer Index 10 (96% over 27bp)
CACATCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGC40.10290712631849756RNA PCR Primer, Index 29 (96% over 25bp)
TCTCCGAGCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA40.10290712631849756TruSeq Adapter, Index 18 (96% over 28bp)
AGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG40.10290712631849756Illumina PCR Primer Index 10 (95% over 24bp)
TTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC40.10290712631849756No Hit
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA40.10290712631849756TruSeq Adapter, Index 11 (96% over 30bp)
AAAAAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC40.10290712631849756No Hit
CATCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT40.10290712631849756TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTT40.10290712631849756TruSeq Adapter, Index 12 (96% over 25bp)
CTTATACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGT40.10290712631849756No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC156.124003E-495.06
CTTTTTT250.004650525357.0000041
TATACAC755.297823E-638.03
TTATACA755.297823E-638.02
CTTATAC755.297823E-638.01
TACACAT851.2512091E-533.529415
ATACACA851.2512091E-533.529414
TCTCCGA1651.3114914E-928.7878781
ACACATC1055.3110525E-527.1428576
CACATCT1107.294053E-525.9090927
CGAGCCC1654.766298E-825.9090925
TCCGAGC1854.467438E-925.6756743
CTCCGAG1706.3710104E-825.1470582
CCGAGCC2001.0273652E-823.7500024
CATCTCC1251.7405426E-422.81
ATCTCCG1200.003968858619.7916682
ACATCTC1400.00968090316.9642878
GAGCCCA2254.8481327E-414.7777791
CGAGACA1951.6184384E-514.6153848
GAGACAC1952.3218435E-414.6153849
Produced by FastQC (version 0.11.2)