FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s88.351000000d85db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s88.351000000d85db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAAACCAACATAAACATTATTGCCCGGCGTACGGGGAAGGACGTCAA19.090909090909092No Hit
TAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAA19.090909090909092No Hit
CCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT19.090909090909092No Hit
ACTGAACGGACTGGAAACACTGGTCATAATCATGGTGGCGAATAAGTACG19.090909090909092No Hit
TAATAGCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGA19.090909090909092No Hit
AAGGTCCATATCTGACTTTTTGTTAACGTATTTAGCCACATAGAAACCAA19.090909090909092No Hit
TTATGGAAGCCAAACATTGGGGATTGAGAAAGAGTAGAAATGCCACAAGC19.090909090909092No Hit
GACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGA19.090909090909092No Hit
GTAAAGCTGATGGAATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGA19.090909090909092No Hit
CCACACCAGAAGCGGCATCAGTGACGACATTAGAAATATCCTTTGCAGTA19.090909090909092No Hit
GATAATCCCAATGGTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)