FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s86.351000000d85a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s86.351000000d85a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1478.184855233853007No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191.0579064587973273No Hit
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC181.0022271714922049RNA PCR Primer, Index 12 (96% over 26bp)
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA181.0022271714922049Illumina PCR Primer Index 5 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGC160.8908685968819599Illumina PCR Primer Index 10 (96% over 26bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC140.779510022271715RNA PCR Primer, Index 41 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT100.5567928730512249No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.5567928730512249No Hit
GAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAA90.5011135857461024Illumina PCR Primer Index 10 (96% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.4454342984409799No Hit
GAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA80.4454342984409799Illumina PCR Primer Index 10 (96% over 29bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA70.3897550111358575RNA PCR Primer, Index 17 (96% over 28bp)
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA70.3897550111358575TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT50.27839643652561247RNA PCR Primer, Index 29 (95% over 23bp)
AGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA40.22271714922048996Illumina PCR Primer Index 10 (96% over 29bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16703786191536749No Hit
AAAAAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT30.16703786191536749Illumina PCR Primer Index 10 (95% over 21bp)
GATCGGAAGAGCCCACGAGACCCTAAGACACCTCGTATGCCGTCTTCTGC30.16703786191536749Illumina PCR Primer Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGT30.16703786191536749No Hit
GATCGGAAGAGCCCACGAGACGCGTAGTAACCTCGTATGCCGTCTTCTGC30.16703786191536749Illumina PCR Primer Index 12 (95% over 24bp)
AAAAAAAAAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCC20.11135857461024498No Hit
TCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11135857461024498No Hit
AAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT20.11135857461024498No Hit
CATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT20.11135857461024498TruSeq Adapter, Index 12 (96% over 27bp)
GCACCCCTGTCGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA20.11135857461024498No Hit
CGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG20.11135857461024498RNA PCR Primer, Index 12 (96% over 29bp)
AAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC20.11135857461024498No Hit
TCTCCGAGCCCACTAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG20.11135857461024498TruSeq Adapter, Index 12 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA20.11135857461024498No Hit
CTTATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT20.11135857461024498No Hit
AGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG20.11135857461024498RNA PCR Primer, Index 17 (95% over 24bp)
AGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.11135857461024498TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCCACGATACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG20.11135857461024498Illumina PCR Primer Index 12 (96% over 29bp)
CCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20.11135857461024498Illumina PCR Primer Index 10 (96% over 29bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGCGTAGT20.11135857461024498No Hit
TTATACACATCTCCGAGCCCACGAACGCGTAGTAATCTCGTATGCCGTCT20.11135857461024498Illumina PCR Primer Index 12 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCAGCCAATCTCGTATGCCGTCTTCT20.11135857461024498RNA PCR Primer, Index 4 (95% over 23bp)
AAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT20.11135857461024498TruSeq Adapter, Index 12 (95% over 24bp)
CCTTGGCGACGTCGCCGGGTAGCAAAAGCGGCTCGGCGACGCGAAGCACG20.11135857461024498No Hit
AAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC20.11135857461024498No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAA20.11135857461024498No Hit
CACCCCGGGATAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTAT20.11135857461024498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAA20.11135857461024498No Hit
AAAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC20.11135857461024498Illumina PCR Primer Index 10 (95% over 23bp)
ACACATCTCCGAGCCAACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT20.11135857461024498RNA PCR Primer, Index 10 (95% over 24bp)
CATCTACGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT20.11135857461024498Illumina PCR Primer Index 10 (96% over 27bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG20.11135857461024498TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT20.11135857461024498TruSeq Adapter, Index 12 (95% over 22bp)
CCCCTTGGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC20.11135857461024498No Hit
GTGTCCGCCATGGCCCACAAGGTCTACCTCATCGAGGAACCCCTGGCGGC20.11135857461024498No Hit
TCTCCGAGCCCACTAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG20.11135857461024498RNA PCR Primer, Index 41 (96% over 29bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11135857461024498No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA20.11135857461024498No Hit
CCCCTGTTTAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC20.11135857461024498No Hit
CCCCTTTTGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC20.11135857461024498No Hit
AAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT20.11135857461024498No Hit
GATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11135857461024498No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11135857461024498No Hit
CCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTC20.11135857461024498No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAA20.11135857461024498No Hit
TCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG20.11135857461024498TruSeq Adapter, Index 12 (96% over 29bp)
GCACCCTGTGGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTAT20.11135857461024498No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT20.11135857461024498Illumina PCR Primer Index 10 (95% over 24bp)
AAAAAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCC20.11135857461024498No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT20.11135857461024498RNA PCR Primer, Index 29 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCCC155.5593136E-495.01
TCTCCGA300.00869813447.51
CTCCGAG300.00869813447.52
AGATCGG557.098439E-525.90909212-13
CGGAAGA601.2837572E-423.7516-17
ATCGGAA601.2837572E-423.7514-15
GAAGAGC652.2111477E-421.92307518-19
AGAGCCC652.2111477E-421.92307520-21
CACGAGA950.002851617315.026-27
CCCACGA1100.007571415512.95454624-25
Produced by FastQC (version 0.11.2)