FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s85.351000000d858b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s85.351000000d858b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350708
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT1887174.337615854706866No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT1477323.395585270259461RNA PCR Primer, Index 29 (95% over 23bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG1067792.4542901982849687RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT833501.9157801442891593RNA PCR Primer, Index 29 (96% over 25bp)
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT350790.8062825636655RNA PCR Primer, Index 29 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC340060.7816199110581542No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA273390.6283804842798RNA PCR Primer, Index 29 (96% over 30bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTG219660.5048833431248431Illumina PCR Primer Index 10 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT199380.4582702401540163RNA PCR Primer, Index 29 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163110.37490449830234523No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161840.371985433175474No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159450.3664920743934091No Hit
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC102810.23630636668790458Illumina PCR Primer Index 10 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93740.2154591850337922No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA85640.19684152556319567No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA74390.17098366518736718No Hit
GGTACTATGATTTCACCGAGTCTCTCGTTGAGACAGTGCCCAAATCGTTA52100.11975062449605903No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGGTTA47140.10835018116591598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG111300.048.9086155
TTCCGAT357850.047.015762
CCGATCT361650.045.917574
TCCGATC374500.044.6718033
CTTCCGA380100.044.4153631
CGATCTA53800.041.8496635
GATCTGG44900.041.4701546
CGATCTT152500.039.339555
GATCTAG20800.037.9088556
GATCTTT120450.037.8976676
TCTTCCG196800.036.7113235
GTGCTCT200300.036.6477051
TGCTCTT199100.036.621242
CGATCTC84200.035.879055
CTCTTCC205350.035.3909844
GCTCTTC213650.034.2606543
ATCTTTT129550.033.9874277
GATCTGC43050.033.983926
GATCTGA39350.030.66096
ATCTGGT14500.030.464287
Produced by FastQC (version 0.11.2)