HG5M3BCX2_l01n01_r3zt2s84.351000000d857e.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l01n01_r3zt2s84.351000000d857e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1315
Sequences flagged as poor quality	0
Sequence length	101
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	66	5.019011406844107	Illumina PCR Primer Index 7 (96% over 27bp)
GAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA	56	4.258555133079848	RNA PCR Primer, Index 41 (96% over 31bp)
TCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	42	3.193916349809886	RNA PCR Primer, Index 41 (96% over 31bp)
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	37	2.8136882129277567	Illumina PCR Primer Index 7 (96% over 27bp)
AGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA	14	1.064638783269962	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTG	14	1.064638783269962	TruSeq Adapter, Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT	13	0.988593155893536	No Hit
TCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	12	0.9125475285171103	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	11	0.8365019011406843	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGT	10	0.7604562737642585	RNA PCR Primer, Index 41 (95% over 21bp)
ACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	10	0.7604562737642585	RNA PCR Primer, Index 41 (96% over 26bp)
CCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAA	10	0.7604562737642585	Illumina PCR Primer Index 7 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT	10	0.7604562737642585	RNA PCR Primer, Index 29 (96% over 25bp)
TCTCCGAGCCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	8	0.6083650190114068	Illumina PCR Primer Index 3 (96% over 26bp)
ATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTT	7	0.532319391634981	RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	7	0.532319391634981	RNA PCR Primer, Index 41 (96% over 31bp)
CACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG	6	0.45627376425855515	Illumina PCR Primer Index 3 (95% over 23bp)
TCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	6	0.45627376425855515	Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	6	0.45627376425855515	RNA PCR Primer, Index 41 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	6	0.45627376425855515	RNA PCR Primer, Index 29 (95% over 23bp)
TCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	6	0.45627376425855515	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	5	0.38022813688212925	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	5	0.38022813688212925	RNA PCR Primer, Index 41 (96% over 29bp)
CTTATACACATCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTC	5	0.38022813688212925	RNA PCR Primer, Index 41 (95% over 22bp)
ATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTC	5	0.38022813688212925	Illumina PCR Primer Index 3 (95% over 21bp)
TCTCCGAGCCCAGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	5	0.38022813688212925	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	5	0.38022813688212925	Illumina PCR Primer Index 7 (96% over 27bp)
ACACATCTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	4	0.3041825095057034	RNA PCR Primer, Index 41 (96% over 26bp)
CTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.3041825095057034	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	4	0.3041825095057034	RNA PCR Primer, Index 41 (95% over 21bp)
CTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.3041825095057034	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTG	4	0.3041825095057034	TruSeq Adapter, Index 7 (96% over 26bp)
CATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.3041825095057034	Illumina PCR Primer Index 7 (96% over 25bp)
TCTCCGAGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.3041825095057034	Illumina PCR Primer Index 3 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTC	3	0.22813688212927757	RNA PCR Primer, Index 41 (95% over 22bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTCGACGT	3	0.22813688212927757	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	3	0.22813688212927757	No Hit
CTTATACACATCTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGT	3	0.22813688212927757	RNA PCR Primer, Index 41 (95% over 21bp)
CGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAA	3	0.22813688212927757	Illumina PCR Primer Index 7 (96% over 27bp)
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT	3	0.22813688212927757	RNA PCR Primer, Index 29 (96% over 28bp)
GTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGT	3	0.22813688212927757	No Hit
TCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.22813688212927757	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	3	0.22813688212927757	Illumina PCR Primer Index 7 (96% over 26bp)
TCTCCAAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.22813688212927757	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	3	0.22813688212927757	RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	3	0.22813688212927757	RNA PCR Primer, Index 41 (95% over 24bp)
ATCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTG	3	0.22813688212927757	No Hit
CTTATACACATCTGCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	3	0.22813688212927757	RNA PCR Primer, Index 41 (95% over 21bp)
CTTATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	3	0.22813688212927757	RNA PCR Primer, Index 41 (95% over 21bp)
TCTCCGAGCCCCGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.22813688212927757	Illumina PCR Primer Index 3 (96% over 27bp)
CATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	3	0.22813688212927757	RNA PCR Primer, Index 41 (96% over 29bp)
GATTTGGGCTACACACGTGCTACAATGGACAATACAAAGGGCAGCGAAAC	3	0.22813688212927757	No Hit
CCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	3	0.22813688212927757	RNA PCR Primer, Index 41 (96% over 31bp)
TTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.1520912547528517	Illumina PCR Primer Index 7 (96% over 27bp)
GAATAGGCCCAGGCGACTGTTTACCAAAAACACAGGTCTCTGCTAATTCG	2	0.1520912547528517	No Hit
TCTCCGAGCCCACGTGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGGGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACCTCATCTCGTATGCCGT	2	0.1520912547528517	No Hit
CTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.1520912547528517	Illumina PCR Primer Index 7 (96% over 27bp)
CACATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTG	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 27bp)
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGT	2	0.1520912547528517	No Hit
TCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTG	2	0.1520912547528517	No Hit
ATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 30bp)
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATC	2	0.1520912547528517	No Hit
CATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGGACTCGACGTCATCTCGTATGCCGTCTTC	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 25bp)
CCCTAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTA	2	0.1520912547528517	No Hit
ATACACATCTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2	0.1520912547528517	RNA PCR Primer, Index 41 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTCCACGTAATCTCGTATGCCGT	2	0.1520912547528517	No Hit
TTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTC	2	0.1520912547528517	No Hit
CCGTAGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCG	2	0.1520912547528517	No Hit
CATCCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 30bp)
TCCGAGCCCACGAGACTCGACGTCAACTCGTATGCCGTCTTCTGCTTGAA	2	0.1520912547528517	TruSeq Adapter, Index 7 (96% over 26bp)
TCTCCAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.1520912547528517	Illumina PCR Primer Index 7 (96% over 27bp)
GTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACT	2	0.1520912547528517	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCCTCTGCTTG	2	0.1520912547528517	TruSeq Adapter, Index 7 (96% over 26bp)
TATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCT	2	0.1520912547528517	RNA PCR Primer, Index 41 (95% over 23bp)
CTTATACACATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGT	2	0.1520912547528517	RNA PCR Primer, Index 41 (95% over 21bp)
GGCTCGGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACT	2	0.1520912547528517	No Hit
CCCCTTATGAGCTGGGCTACACACGTGCTACAATGGATGGTACAACGAGT	2	0.1520912547528517	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	2	0.1520912547528517	Illumina PCR Primer Index 7 (95% over 22bp)
TCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTGAA	2	0.1520912547528517	TruSeq Adapter, Index 7 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTCCAGCTCATCTCGTATGCCGTCTT	2	0.1520912547528517	Illumina PCR Primer Index 7 (95% over 24bp)
GGGTTGTAGGACTCAATGTCGGGATTGACCGTTTAAGGTGAACACCCTGG	2	0.1520912547528517	No Hit
ACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 26bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1520912547528517	No Hit
CATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGT	2	0.1520912547528517	No Hit
TCTCCGAGCCCACGAGACTCGACGTCAACTCGTATGCCGTCTTCTGCTTG	2	0.1520912547528517	TruSeq Adapter, Index 7 (96% over 26bp)
CATTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.1520912547528517	Illumina PCR Primer Index 7 (96% over 26bp)
ACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 26bp)
GATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAGTTGCAGACTA	2	0.1520912547528517	No Hit
CCTTTGTATTGTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCA	2	0.1520912547528517	No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGTCCTCCC	2	0.1520912547528517	No Hit
CATCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.1520912547528517	Illumina PCR Primer Index 7 (96% over 25bp)
TCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.1520912547528517	Illumina PCR Primer Index 3 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTCATCTCGTATGCCGTCTT	2	0.1520912547528517	TruSeq Adapter, Index 7 (95% over 23bp)
TATCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.1520912547528517	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACAAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.1520912547528517	Illumina PCR Primer Index 7 (96% over 27bp)
CCCTGGGAAGGGTGGCCAGAGCAGGTGATAGCCCTGTAACCGACCGAGCG	2	0.1520912547528517	No Hit
TCTCCGAGCCCATGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.1520912547528517	RNA PCR Primer, Index 41 (96% over 31bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCACGA	65	7.3252544E-5	36.53846	9
GCCCACG	65	0.0039692456	29.230766	8
AGCCCAC	70	0.0057088467	27.142859	7
GTCATCT	60	1.15634124E-4	23.75	24-25
CATCTCG	60	1.15634124E-4	23.75	26-27
TCATCTC	60	1.15634124E-4	23.75	24-25
CGTCATC	65	1.9929156E-4	21.923075	22-23
ACGTCAT	65	1.9929156E-4	21.923075	22-23
ATCTCGT	70	3.294951E-4	20.357143	26-27
TGCCGTC	70	3.294951E-4	20.357143	34-35
TCTCGTA	70	3.294951E-4	20.357143	28-29
TCGTATG	70	3.294951E-4	20.357143	30-31
GCCGTCT	70	3.294951E-4	20.357143	36-37
AGACTCG	70	3.294951E-4	20.357143	14-15
GTATGCC	75	5.256036E-4	18.999998	32-33
TATGCCG	75	5.256036E-4	18.999998	32-33
GAAAAAA	75	5.256036E-4	18.999998	50-51
TTCTGCT	75	5.256036E-4	18.999998	42-43
CCGTCTT	75	5.256036E-4	18.999998	36-37
TCTGCTT	75	5.256036E-4	18.999998	42-43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content