FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s83.351000000d8555.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s83.351000000d8555.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6285214
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT2165333.445117381842527No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT1636152.6031730980043RNA PCR Primer, Index 29 (95% over 23bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG1263492.010257725512608RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT951271.5135045521123067RNA PCR Primer, Index 29 (96% over 25bp)
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT431900.6871683287156173RNA PCR Primer, Index 29 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC360650.5738070334597994No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA322030.512361233841839RNA PCR Primer, Index 29 (96% over 30bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTG236970.37702773525292854Illumina PCR Primer Index 10 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT216380.3442683097186508RNA PCR Primer, Index 29 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202510.3222006442421849No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123320.196206525346631No Hit
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC106550.16952485627378797Illumina PCR Primer Index 10 (96% over 26bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101480.1614583051587424No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT84170.1339174767955395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68510.1090018573751029No Hit
TCATTATGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT65650.1044514952076413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT151150.029.004757
GATCTTT164350.027.4563796
CCGATCT388550.027.1268754
CTTCCGA397350.026.898451
CGATCTT194900.026.5159035
TTCCGAT401700.026.4516962
TCCGATC399200.026.4388713
TCTTTTT205900.021.592118
GTATAAG17400.020.7462658
TAGGACT49400.020.6728764
GCTCTTC299600.020.6581783
TCTTCCG299950.020.5865635
CTTTTTT214200.020.5780439
CTCTTCC306450.020.2429084
ATCTAGG51300.020.0189171
CTAGGAC50500.019.8463423
GTGCTCT312600.019.817991
TGCTCTT324250.019.1463052
GTATAGT40500.018.5483931
AGGACTA53750.018.4695875
Produced by FastQC (version 0.11.2)