HG5M3BCX2_l01n01_r3zt2s82.351000000d853b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l01n01_r3zt2s82.351000000d853b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2194
Sequences flagged as poor quality	0
Sequence length	101
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA	39	1.7775752051048315	Illumina PCR Primer Index 5 (96% over 28bp)
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA	26	1.1850501367365542	Illumina PCR Primer Index 9 (100% over 27bp)
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA	24	1.0938924339106655	Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG	22	1.0027347310847767	Illumina PCR Primer Index 11 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC	19	0.8659981768459436	RNA PCR Primer, Index 12 (96% over 26bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA	17	0.7748404740200547	RNA PCR Primer, Index 40 (96% over 29bp)
TCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAA	16	0.7292616226071102	TruSeq Adapter, Index 9 (96% over 28bp)
GATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC	15	0.683682771194166	RNA PCR Primer, Index 47 (96% over 26bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA	15	0.683682771194166	Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG	11	0.5013673655423884	TruSeq Adapter, Index 11 (96% over 30bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA	10	0.4557885141294439	TruSeq Adapter, Index 11 (96% over 30bp)
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA	10	0.4557885141294439	TruSeq Adapter, Index 12 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	0.4557885141294439	No Hit
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA	10	0.4557885141294439	Illumina PCR Primer Index 11 (96% over 29bp)
ACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT	8	0.3646308113035551	Illumina PCR Primer Index 12 (95% over 24bp)
TCTCCGAGCCAACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG	8	0.3646308113035551	RNA PCR Primer, Index 40 (96% over 29bp)
CTTATACACATCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	8	0.3646308113035551	No Hit
TCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG	7	0.31905195989061075	Illumina PCR Primer Index 12 (96% over 29bp)
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA	7	0.31905195989061075	Illumina PCR Primer Index 11 (96% over 29bp)
TCTCAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG	7	0.31905195989061075	Illumina PCR Primer Index 11 (96% over 29bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTACGCTG	6	0.27347310847766637	No Hit
GATCGGAAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGC	6	0.27347310847766637	Illumina PCR Primer Index 11 (96% over 26bp)
TCTCCGAGCACACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG	6	0.27347310847766637	TruSeq Adapter, Index 11 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	6	0.27347310847766637	RNA PCR Primer, Index 29 (95% over 23bp)
TCTCCGAGCCCACTAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG	6	0.27347310847766637	Illumina PCR Primer Index 11 (96% over 29bp)
TCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG	6	0.27347310847766637	Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA	6	0.27347310847766637	TruSeq Adapter, Index 18 (96% over 28bp)
CATCTCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT	6	0.27347310847766637	TruSeq Adapter, Index 12 (96% over 28bp)
TCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	6	0.27347310847766637	Illumina PCR Primer Index 9 (96% over 28bp)
TCTCCGAGCCCACTAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG	6	0.27347310847766637	TruSeq Adapter, Index 6 (96% over 28bp)
CCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAA	5	0.22789425706472194	RNA PCR Primer, Index 12 (96% over 29bp)
TCTCCGAGCCCACGATACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG	5	0.22789425706472194	Illumina PCR Primer Index 12 (96% over 29bp)
ATACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	5	0.22789425706472194	TruSeq Adapter, Index 9 (95% over 21bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG	5	0.22789425706472194	TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGATACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	5	0.22789425706472194	Illumina PCR Primer Index 9 (96% over 28bp)
CCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAA	5	0.22789425706472194	Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT	5	0.22789425706472194	No Hit
TCTCCGAGCACACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG	5	0.22789425706472194	RNA PCR Primer, Index 40 (96% over 29bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG	5	0.22789425706472194	TruSeq Adapter, Index 12 (96% over 29bp)
AGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA	5	0.22789425706472194	RNA PCR Primer, Index 47 (96% over 29bp)
CATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT	4	0.18231540565177756	TruSeq Adapter, Index 9 (96% over 26bp)
TCTCCGAGCCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT	4	0.18231540565177756	Illumina PCR Primer Index 12 (96% over 28bp)
TCTCCGAGCACACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	Illumina PCR Primer Index 12 (96% over 29bp)
ACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT	4	0.18231540565177756	Illumina PCR Primer Index 5 (95% over 23bp)
CTTATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	4	0.18231540565177756	No Hit
TCTACGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	RNA PCR Primer, Index 40 (96% over 29bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	RNA PCR Primer, Index 47 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTT	4	0.18231540565177756	TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGT	4	0.18231540565177756	No Hit
TCTCCTAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	TruSeq Adapter, Index 12 (96% over 29bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA	4	0.18231540565177756	RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGATCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	Illumina PCR Primer Index 9 (96% over 28bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA	4	0.18231540565177756	RNA PCR Primer, Index 11 (96% over 30bp)
TCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	TruSeq Adapter, Index 9 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTT	4	0.18231540565177756	Illumina PCR Primer Index 12 (95% over 22bp)
TCTCCGAGCCCACTAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCCACGAACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGA	4	0.18231540565177756	Illumina PCR Primer Index 6 (96% over 28bp)
TCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	TruSeq Adapter, Index 11 (96% over 29bp)
TCTCAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	RNA PCR Primer, Index 40 (96% over 29bp)
TCTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	RNA PCR Primer, Index 47 (96% over 29bp)
TCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG	4	0.18231540565177756	TruSeq Adapter, Index 12 (96% over 29bp)
GATCGGAAGAGCCCACGAGACATGCGCAGACCTCGTATGCCGTCTTCTGC	3	0.13673655423883319	Illumina PCR Primer Index 9 (95% over 24bp)
CATCTCCGAGCCAACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCT	3	0.13673655423883319	RNA PCR Primer, Index 40 (96% over 27bp)
TCTCCTAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG	3	0.13673655423883319	Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTC	3	0.13673655423883319	Illumina PCR Primer Index 11 (95% over 21bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC	3	0.13673655423883319	RNA PCR Primer, Index 11 (96% over 27bp)
TCTCCGAGCCCACGATACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG	3	0.13673655423883319	TruSeq Adapter, Index 11 (96% over 29bp)
CCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	3	0.13673655423883319	RNA PCR Primer, Index 40 (96% over 29bp)
CTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA	3	0.13673655423883319	Illumina PCR Primer Index 12 (96% over 29bp)
CATCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCT	3	0.13673655423883319	Illumina PCR Primer Index 11 (96% over 27bp)
CTTATACACATCTCCGAGCCCAGAGACTACGCTGCATCTCGTATGCCGTC	3	0.13673655423883319	No Hit
CATCTCCTAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCT	3	0.13673655423883319	Illumina PCR Primer Index 11 (96% over 27bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.13673655423883319	No Hit
TCTCCGAGCCCACGAGACTACGCTGCAACTCGTATGCCGTCTTCTGCTTG	3	0.13673655423883319	TruSeq Adapter, Index 3 (96% over 26bp)
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG	3	0.13673655423883319	TruSeq Adapter, Index 11 (96% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.13673655423883319	No Hit
TCTCCAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA	3	0.13673655423883319	RNA PCR Primer, Index 47 (96% over 29bp)
ATACACATCTCAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	3	0.13673655423883319	Illumina PCR Primer Index 11 (95% over 22bp)
TCTCCAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA	3	0.13673655423883319	RNA PCR Primer, Index 12 (96% over 29bp)
ACACATCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT	3	0.13673655423883319	Illumina PCR Primer Index 11 (95% over 24bp)
TATCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG	3	0.13673655423883319	Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACAAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG	3	0.13673655423883319	TruSeq Adapter, Index 6 (96% over 28bp)
AGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA	3	0.13673655423883319	Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGA	3	0.13673655423883319	RNA PCR Primer, Index 6 (96% over 28bp)
TCTCCGAGCCCACGATACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG	3	0.13673655423883319	RNA PCR Primer, Index 40 (96% over 29bp)
ATACACATCTCCGAGCCCACGAACGCGTAGTAATCTCGTATGCCGTCTTC	3	0.13673655423883319	Illumina PCR Primer Index 12 (95% over 23bp)
CTCCGAGCCCACGATACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA	3	0.13673655423883319	Illumina PCR Primer Index 9 (96% over 28bp)
CATCTCAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCT	3	0.13673655423883319	RNA PCR Primer, Index 40 (96% over 27bp)
AGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA	3	0.13673655423883319	TruSeq Adapter, Index 6 (96% over 28bp)
TCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA	3	0.13673655423883319	Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG	3	0.13673655423883319	RNA PCR Primer, Index 29 (96% over 30bp)
TCTCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA	3	0.13673655423883319	Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT	3	0.13673655423883319	RNA PCR Primer, Index 47 (96% over 28bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA	3	0.13673655423883319	TruSeq Adapter, Index 11 (96% over 30bp)
CATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT	3	0.13673655423883319	Illumina PCR Primer Index 9 (96% over 26bp)
GAGCCCACGAGACGCGTAGTAACCTCGTATGCCGTCTTCTGCTTGAAAAA	3	0.13673655423883319	Illumina PCR Primer Index 12 (96% over 27bp)
TCTCCGAGCCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTT	3	0.13673655423883319	TruSeq Adapter, Index 18 (96% over 27bp)
ATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT	3	0.13673655423883319	RNA PCR Primer, Index 12 (96% over 27bp)
CACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG	3	0.13673655423883319	RNA PCR Primer, Index 47 (96% over 25bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCAG	15	5.748278E-4	95.0	2
CATCTCA	15	5.748278E-4	95.0	1
CAGAGCC	15	5.748278E-4	95.0	6
TCAGAGC	15	5.748278E-4	95.0	5
CTCAGAG	15	5.748278E-4	95.0	4
TCTCAGA	15	5.748278E-4	95.0	3
TATACAC	35	2.395118E-6	67.85715	3
TTATACA	35	2.395118E-6	67.85715	2
CTTATAC	35	2.395118E-6	67.85715	1
TACACAT	45	1.0652404E-5	52.777782	5
ATACACA	45	1.0652404E-5	52.777782	4
CACATCT	50	1.9889296E-5	47.5	7
ACACATC	55	3.4962453E-5	43.18182	6
CATCTCC	45	7.63518E-4	42.222225	9
ACATCTC	50	0.0012830413	38.0	8
AAAAAAA	1050	1.3762474E-8	5.428571	56-57

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content