FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s81.351000000d8504.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s81.351000000d8504.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3737
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA1483.9603960396039604Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA992.6491838373026493Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC681.8196414236018195Illumina PCR Primer Index 10 (96% over 25bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG661.7661225582017663Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGCT461.2309339042012308No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA411.097136740701097Illumina PCR Primer Index 10 (96% over 28bp)
TCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG411.097136740701097Illumina PCR Primer Index 10 (96% over 28bp)
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA381.0168584426010168Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG320.8563018464008563TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG270.7225046829007226Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG250.668985817500669TruSeq Adapter, Index 10 (96% over 28bp)
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA240.6422263848006422Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG230.6154669521006154Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT220.5887075194005887No Hit
ACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCT220.5887075194005887RNA PCR Primer, Index 10 (95% over 23bp)
TCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG210.561948086700562Illumina PCR Primer Index 10 (96% over 28bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA200.5351886540005352Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGT190.5084292213005084No Hit
TCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG190.5084292213005084TruSeq Adapter, Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.5084292213005084No Hit
ATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT180.48166978860048165Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT180.48166978860048165No Hit
CTTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT170.45491035590045487No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG170.45491035590045487Illumina PCR Primer Index 11 (96% over 29bp)
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA160.42815092320042814Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT150.4013914905004014Illumina PCR Primer Index 10 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT150.4013914905004014Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTT150.4013914905004014TruSeq Adapter, Index 10 (95% over 21bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA150.4013914905004014Illumina PCR Primer Index 11 (100% over 29bp)
CATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT140.3746320578003747Illumina PCR Primer Index 10 (96% over 27bp)
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA140.3746320578003747Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT140.3746320578003747RNA PCR Primer, Index 29 (95% over 23bp)
CATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT140.3746320578003747Illumina PCR Primer Index 10 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT140.3746320578003747No Hit
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT140.3746320578003747RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA130.34787262510034783RNA PCR Primer, Index 29 (96% over 28bp)
CATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT110.29435375970029437Illumina PCR Primer Index 10 (96% over 26bp)
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT110.29435375970029437TruSeq Adapter, Index 10 (96% over 26bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC100.2675943270002676RNA PCR Primer, Index 11 (96% over 27bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2675943270002676No Hit
CTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.2675943270002676Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.2675943270002676RNA PCR Primer, Index 10 (96% over 28bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA100.2675943270002676Illumina PCR Primer Index 11 (96% over 29bp)
CTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA90.24083489430024083Illumina PCR Primer Index 10 (96% over 28bp)
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA90.24083489430024083Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT90.24083489430024083TruSeq Adapter, Index 10 (96% over 26bp)
CATCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT90.24083489430024083RNA PCR Primer, Index 29 (96% over 27bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT80.21407546160021407Illumina PCR Primer Index 10 (96% over 26bp)
CTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.21407546160021407Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.21407546160021407Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG80.21407546160021407TruSeq Adapter, Index 10 (96% over 28bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGC80.21407546160021407No Hit
TATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG80.21407546160021407Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTT80.21407546160021407TruSeq Adapter, Index 10 (95% over 21bp)
CTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGA70.18731602890018734RNA PCR Primer, Index 12 (96% over 26bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA70.18731602890018734No Hit
TCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.18731602890018734Illumina PCR Primer Index 10 (96% over 28bp)
CATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT60.16055659620016055Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCTAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG60.16055659620016055Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGT60.16055659620016055No Hit
TCTCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTG60.16055659620016055TruSeq Adapter, Index 12 (96% over 26bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.16055659620016055RNA PCR Primer, Index 29 (96% over 30bp)
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA60.16055659620016055Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT60.16055659620016055Illumina PCR Primer Index 10 (95% over 21bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.16055659620016055No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCGCTAATCTCGTATGCCGT60.16055659620016055No Hit
CTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.16055659620016055RNA PCR Primer, Index 10 (96% over 28bp)
TTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.16055659620016055Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.16055659620016055Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.16055659620016055RNA PCR Primer, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTG60.16055659620016055RNA PCR Primer, Index 10 (95% over 24bp)
TCTCCGAGCCCACCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT60.16055659620016055Illumina PCR Primer Index 10 (96% over 27bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTACGCTG50.1337971635001338No Hit
CTTATACACATCTCAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT50.1337971635001338No Hit
ACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.1337971635001338Illumina PCR Primer Index 10 (95% over 23bp)
TCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.1337971635001338RNA PCR Primer, Index 29 (96% over 28bp)
CACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG50.1337971635001338TruSeq Adapter, Index 10 (95% over 24bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.1337971635001338TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGAACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.1337971635001338Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTCTCGCTAATCTCGTATGCCGT50.1337971635001338No Hit
ACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC50.1337971635001338RNA PCR Primer, Index 29 (96% over 25bp)
TCTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTG50.1337971635001338TruSeq Adapter, Index 12 (96% over 26bp)
ACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.1337971635001338Illumina PCR Primer Index 10 (95% over 23bp)
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA50.1337971635001338Illumina PCR Primer Index 10 (96% over 28bp)
CACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG40.10703773080010703Illumina PCR Primer Index 10 (95% over 24bp)
GATCGGAAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGC40.10703773080010703Illumina PCR Primer Index 11 (96% over 26bp)
TCTCTGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.10703773080010703Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCT40.10703773080010703RNA PCR Primer, Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCCGCTAATCTCGTATGCCGT40.10703773080010703No Hit
ATACACATCTCCGAGCCCACGAGACTCTCGCTAATCTCGTATGCCGTCTT40.10703773080010703TruSeq Adapter, Index 10 (95% over 21bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10703773080010703No Hit
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.10703773080010703TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGATACACTCGCTAACCTCGTATGCCGTCTTCTGCTTG40.10703773080010703RNA PCR Primer, Index 29 (96% over 27bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA40.10703773080010703TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10703773080010703RNA PCR Primer, Index 29 (96% over 28bp)
TTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTC40.10703773080010703No Hit
CTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA40.10703773080010703TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10703773080010703Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT40.10703773080010703RNA PCR Primer, Index 29 (96% over 25bp)
TCTCCGAGCCCACAAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.10703773080010703Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10703773080010703RNA PCR Primer, Index 29 (96% over 28bp)
CTTATACACATCTCGGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT40.10703773080010703No Hit
CATCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCT40.10703773080010703TruSeq Adapter, Index 10 (96% over 26bp)
AGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG40.10703773080010703Illumina PCR Primer Index 10 (95% over 24bp)
TTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC40.10703773080010703No Hit
CATCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT40.10703773080010703Illumina PCR Primer Index 10 (96% over 27bp)
CATCTTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC40.10703773080010703Illumina PCR Primer Index 10 (96% over 25bp)
CATCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT40.10703773080010703TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCGCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.10703773080010703Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.10703773080010703TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTGA40.10703773080010703RNA PCR Primer, Index 12 (96% over 26bp)
TCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.10703773080010703Illumina PCR Primer Index 10 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCA250.004635797357.09
CTTATAC901.2369128E-1047.5000041
TATACAC952.0918378E-1045.03
TTATACA1003.45608E-1042.752
ATACACA1003.45608E-1042.754
TACACAT1055.6024874E-1040.7142835
ACACATC1202.075467E-935.6256
CACATCT1253.09592E-934.1999977
ACATCTC1409.37689E-930.5357118
CGAGCCC1552.5311238E-827.5806455
TCTCCGA1803.2341632E-926.3888911
CTCCGAG1854.3364707E-925.6756742
TCCGAGC2105.275069E-1024.880953
ATCTCCG1359.331658E-624.629632
CCGAGCC1957.610652E-924.3589744
CATCTCC1401.23790305E-523.7499981
AGCCCAC2003.0053343E-721.3757
GCCCACG1905.1986062E-620.08
GAGCCCA1905.1986062E-620.06
ACTCGCT2253.6379788E-1216.8888918-19
Produced by FastQC (version 0.11.2)