FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s80.351000000d84f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s80.351000000d84f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG21.4598540145985401TruSeq Adapter, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT21.4598540145985401TruSeq Adapter, Index 6 (95% over 21bp)
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC21.4598540145985401No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA21.4598540145985401No Hit
AGCCAGCTTGCGGGAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAACC10.7299270072992701No Hit
TCATCTGTGTGCCCGTTAAAGTGCCCGGTCCGGATCCTAAAGATCCTACC10.7299270072992701No Hit
CTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGACGACCAGGGCGA10.7299270072992701No Hit
CCACTGACTTCTTTCTTGAGGATTGGGACGCCATCTTGGCCTCCCTTGAA10.7299270072992701No Hit
AGAGAAGACAAGGTAGGATTTTCCCAGAAGGTAAAATGTGGAAACTCACC10.7299270072992701No Hit
GGGGAGTCGCCTGACCCGGTTCCAAGCCAGGCTGATTTACCGTTGCTAAC10.7299270072992701No Hit
TTGTTAATGTTTTCCATTATCTTTTTTTTTCGTTATTTGTCTTCTCGAAT10.7299270072992701No Hit
GTTCTCATCGGCGGCGAACTCGGCGGGATCGGCAGCACCACGCGCATCTG10.7299270072992701No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA10.7299270072992701No Hit
ATTTTGAGCTGCATTCCCAAACAACTCGACTCTTAGAAAGTGTATCACAA10.7299270072992701No Hit
GGGGTGTCTGTAGTGGAGCATAGCCAGGAACAGCCATCCCTCTAGGCTCA10.7299270072992701No Hit
AATCAGAATGAACCGCAACTTCGGGATGAAAATGCTCACAATGACAAATC10.7299270072992701No Hit
CCATAACTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGT10.7299270072992701No Hit
CAATAACTTGGGAGACAATGCGTTGCTTTGTACTTGGAGCCTCCGGACTA10.7299270072992701No Hit
GGTAGGACTACCCACTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA10.7299270072992701No Hit
GTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCC10.7299270072992701No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCCCCAATATTTAGCCTTAGATG10.7299270072992701No Hit
AATACAAATTATGGATCGTTGGCTAAATCGCGTTGCTGTTGTCACTGGCG10.7299270072992701No Hit
TATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACAT10.7299270072992701No Hit
CAGCAGCAACAGCTACCCCAATCACAACAACAACTGCAGCAGCAGCAACA10.7299270072992701No Hit
TGATGGGTCCCTGCTGTTGCTGTTGGGGCAATTGCTGTTGCATCTGCATG10.7299270072992701No Hit
GTCCTCTGTGCCGGTCAAGTCGTGGGGCATCCAGTTTCGCGAAGTGGTCA10.7299270072992701No Hit
GCTTTGGTTTCCCGCGAAGCGACTGAGAGAGCCATAAAAGTAGCTACACC10.7299270072992701No Hit
TGATAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT10.7299270072992701No Hit
AAATAATTGTCTGAATATGGAATGTCATACCTCACTGAGCTCGTAATAAA10.7299270072992701No Hit
CTTCAAGTCTGTTTTATCAGAGACTAGGATTGCAACCCCTGCTTTTTTTG10.7299270072992701No Hit
GTTTGGGAATGCAGCTCAAAATGGGTGGTAAATGCCATCTAAGGCTAAAT10.7299270072992701No Hit
GGAGGGTAGTCGGAACCGAAGAAGACTCAAAGCGAACCAAACAGGCAAAA10.7299270072992701No Hit
TTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGT10.7299270072992701No Hit
CATCGCGTCCCTATGGCGTGTGCTGACCGCGACCGACCTGTGCGCCCACC10.7299270072992701No Hit
TTACAATTCAATCCCTGCATGGACATGGCAGACAGTTCGGCTGACGATGA10.7299270072992701No Hit
TTTCACACCCGGTCGTACTCATAACCGCATCAGGTCCTCAAGGTGAACAG10.7299270072992701No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGA10.7299270072992701RNA PCR Primer, Index 6 (96% over 28bp)
CCCATCGAATATGAGCTGACGCCCTACGAAATACTCATGGGCGACATCCG10.7299270072992701No Hit
ATCGCTGGCTGTGCGATTTGTTGCGTAATATTCAACTAAATTCATTTGCA10.7299270072992701No Hit
AAGCAGAATTCAGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTG10.7299270072992701No Hit
GTCTATTCTTCTCGAATTTTATGCGGCTGCTGCAGTTGCTTCTTCCACTT10.7299270072992701No Hit
GCTTAAGTTAGTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAA10.7299270072992701No Hit
TAGTTGAACTTGTGCTTCATACGGGTAGTACAACTTACAATTGTGGTTAG10.7299270072992701No Hit
AACATACACATTTCAATTTAACTTTCATACTTGGTTTGCCTTGTTTTTAA10.7299270072992701No Hit
GATTATCACCCTCTATGATGCCCTTTTCCAAGGGACTTAGGCATGGTCCG10.7299270072992701No Hit
ATCATGCACTGCAATGCCCACGAGCAGGTTCATCAGAATGATGGTCACGA10.7299270072992701No Hit
CTGCTCAGGCGAGTAGGCGTGGCTGGGGTTGTTGAAGGAGGCCCGTGAGC10.7299270072992701No Hit
CTTGCCTTTAGTACCTCGCAACGGCTGCGGACGACCAGGGCGAGCGCCAG10.7299270072992701No Hit
ATAACCGGAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAG10.7299270072992701No Hit
NCCTCAGATTGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGG10.7299270072992701No Hit
TGTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCATC10.7299270072992701No Hit
GTACGGCTCCACTTACATAAACACATTCAAACACAATAAGCATTTTACTG10.7299270072992701No Hit
GAGCAGACCAGGGCATTGGTATTGGCCGGGGTGCCCACAACCAGCACCTT10.7299270072992701No Hit
TCAGATAGTAATCAACGCTCTTTTAAAATGTCAACAAGAGAATCTCTACC10.7299270072992701No Hit
TCTCTGTCTGAACATGACAGATCCTTGGACAGAATGACTGCAAGCACTGC10.7299270072992701No Hit
GGCGGTATATGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGA10.7299270072992701No Hit
GCTTCGGACCATGCCTAAGTCCCTTGGAAAAGGGCATCATAGAGGGTGAT10.7299270072992701No Hit
GGATTATCACCCTCTATGATGCCCTTTTCCAAGGGACTTAGGCATGGTCC10.7299270072992701No Hit
CATACGCTCGGCGACAGTTTGAATATTAGACATAATTTATCCTCAAGTAA10.7299270072992701No Hit
CTTATAACCCCGATGCCTAACTTTTCTAAAATTGCCGGCACCTTATAACC10.7299270072992701No Hit
GACATGGGTGCCCTGTATGCTCAGCCCATCGAGGAGATCTGCCAGAAGCA10.7299270072992701No Hit
GTTTACTGTTAAGACTACAACGGTATCTAATCGTTTTCGATACACTAACC10.7299270072992701No Hit
AACTAAGGCTTCTTGCGGTTGCTTTTCCTCTTCATGCTGGTGATATCTGG10.7299270072992701No Hit
GCATATCATGGCCTGCATTTTGGTATTGTTGTTATTGTTATTGTTGTTGC10.7299270072992701No Hit
GCAATTAAAATTGTTGACCACCTACATACCAAAGACGAGCGCCTTTACGC10.7299270072992701No Hit
GGCGACGACATGTAGGGATTGATGCGACCATGGGCACGGTACTGTCTCTT10.7299270072992701No Hit
CATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAG10.7299270072992701No Hit
GTATAATACACAGACGCCTGCGCACAGGACTCCCACACATTTTTGTACCC10.7299270072992701No Hit
TATCAGGATTATCGGGATAATCGTTATTAGAGCGATTACGATTACGGCTC10.7299270072992701No Hit
TACCGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTT10.7299270072992701No Hit
GGGTAGCAGTTATAGCACAAACAAACAAAGCAGCTGCGCGTGTGGGAATA10.7299270072992701No Hit
AGCTGCAGCAGCTGGTGGCCGGTGCTAAGAGCACCTACTCAACGGCCTTA10.7299270072992701No Hit
AACGACATGTCCGCGATCTTTTGCTGGATGGACTGGAGCTTGGCAATGGG10.7299270072992701No Hit
GCGTACCATAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCA10.7299270072992701No Hit
CTTAGAAAGTGTATCACAAAGCTTCGGGGGCTCCATGCCATGTACGGGAT10.7299270072992701No Hit
TCATACATCGCTGAGCTCTTTATTAAATTTACAATTAAAATGTGTTCAAA10.7299270072992701No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAG10.7299270072992701No Hit
GTTTTCGGCTTTTCCGGTCCGGTGTCCCGTGTGTCCTGTGCGTTCGATTG10.7299270072992701No Hit
CCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCA10.7299270072992701No Hit
GGTAATAAGCCTTTCAAAACCGTTATTCTATATGACCATTTTAGCCAAGT10.7299270072992701No Hit
GGTCCGGACATAGTAAGGATTGACAGATTGATGGCGCTTTTCATGATTCT10.7299270072992701No Hit
ATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTT10.7299270072992701RNA PCR Primer, Index 40 (96% over 28bp)
GCGCGAATGTGCCTCTCGTGGCACTTGGACAGCTTGGCCACCGCCTTCTC10.7299270072992701No Hit
GAACGAAAGTTAGAGGTTCGAAGGCGATCAGATACCGCCCTAGTTCTAAC10.7299270072992701No Hit
GCTTAATATTACGTTTAGCCTTGTCCATTTTTCACTCAAATTCTGAGAAG10.7299270072992701No Hit
CCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATG10.7299270072992701No Hit
ACATGGCCTCAAGCATTCTGGCTCTTCTTGTCCTCACATAACAGAGACAC10.7299270072992701No Hit
ACCCAGTCATTTCGGACAAGCGGAACCACTCGTTGTCTCTGTCAGTGTAG10.7299270072992701No Hit
GTACTTGTTCGCTATCGGTCTCTCCCCAATATTTAGCCTTAGATGGCATT10.7299270072992701No Hit
GCATAAGACAGGCATATGAGAAAAACCAATTGGCTAACAATAAAGTACCA10.7299270072992701No Hit
AGTGTAGACTGTACGATTTGCTTTAACGCGCTCTTGAAATTAAAACGAGA10.7299270072992701No Hit
GAGTTGGTCTGCTGGGTCGGCGGTGGGATCAGCGGGCGCTGGATCTCACG10.7299270072992701No Hit
GTGCAGGGCGACCACGTCTTGATATCCACGTCGCACACCTGACCCTTCGG10.7299270072992701No Hit
GTCAATTACATCATCGTAATAGTTGGTTCTGTAGTTGTACATGTATTTGT10.7299270072992701No Hit
CATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTA10.7299270072992701No Hit
TTGCAGCTCCAGGGCTTGCAGCTGCTGAGCCTGCTGCTGAATCACCTCGT10.7299270072992701No Hit
GCTCTACATCTTGGCCCTGCTTAAGCACCCCGCTTTCCGTGTGGGAACTA10.7299270072992701No Hit
AATGGCCACGCCGCCGGGAACTCTCATGCAGATGTGGCCAAACTGAGTTA10.7299270072992701No Hit
AGACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACCTGTC10.7299270072992701No Hit
TTCGATAGGTCGGTGCGAACAAGCGCACACTTTCATACGGAACTTCCCTA10.7299270072992701No Hit
TCGGTGATGCGATCCTCGGCCATGTGCTGGATGTTCCTCAGATCCTCGGC10.7299270072992701No Hit
TGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG10.7299270072992701No Hit
GTATTCGACAACAGACACTCTGCAATGATGATGGTGAAGCCTATACCTGC10.7299270072992701No Hit
CCCTGGTTTGCTTTTCGAGCGACAAGATCTGCCGGCGCTTTGCTGGCTGC10.7299270072992701No Hit
ATTCCGAGGTGGGTCCCGTCGTTAAGAAAAGAAAAGAACTCTCCCCAGGG10.7299270072992701No Hit
ATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAAGTTTTGTCTCGG10.7299270072992701No Hit
CCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATAT10.7299270072992701No Hit
ACTTTATTCGCTCTGCATATAACATTTATCATTCAAAGGTCACGAAAGTT10.7299270072992701No Hit
CTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAAC10.7299270072992701No Hit
TCCTTATCCTAGCGCTGTTTCCTCAGCTCCGTGAAGACCTTTTCGAAGAA10.7299270072992701No Hit
CTGTCAGACTATTTAGCGCACACCGTGGGGTTGACGGGTTGCCAGCTGAT10.7299270072992701No Hit
CGTTGCAACCATGGTAGTCCTAGATACTACAATCAAAAGTTGATAGGGCA10.7299270072992701No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTCCTGCT10.7299270072992701TruSeq Adapter, Index 2 (95% over 24bp)
GGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT10.7299270072992701No Hit
GAGCCGGACTACATGAAGCGGGAGCACAGTTTGGTGCGTCCATTCCAAGG10.7299270072992701No Hit
GCAATATCCTTCTTCTGTACCTCAGCTTCAAACTTCTCCATATCCTCCTT10.7299270072992701No Hit
CTGCTACTTCTGGGCGTACCAAGACGCCGGGCCGTTAGACGAGGAGGCAG10.7299270072992701No Hit
GGTATCATCTGCAACATTGGATCCGTCACTGGATTCAATGCCATCTACCA10.7299270072992701No Hit
AGCCAGACCTAGCCAAATTTTGCAAAGCAAAATTTATGGCAGGTCGGCCG10.7299270072992701No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT10.7299270072992701No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG10.7299270072992701No Hit
AACCTGCCCACTGAAAATTTTAAATTGCCGCAGTATTTTGACTGTGCAAA10.7299270072992701No Hit
GGCATAATACTGCGGCACCTTACTGCCGAACTGCTTGTCCTTCAGCTTTT10.7299270072992701No Hit
GATTTGAGGCACCGCACAGTACAGCATCTCAAGAGGCCTACCAGAATAAT10.7299270072992701No Hit
GGGAGGTAGTGACGAAAAAATAACAATACAGGACTCATATCCGAGGCCCT10.7299270072992701No Hit
TAATCGGTCGTCAGCCAACGTGAGAGTGTCAAAAACGATAAACCAACCAT10.7299270072992701No Hit
GGTCCTCCCTGTCCGTGTGGGTGCCCCAGTCCAAAGCCACCAAAGGCAGG10.7299270072992701No Hit
GGCGTGGAGAGCGGAGGAAAGGGAAAGGCGCCCGGTGCAAATTGCTGTCT10.7299270072992701No Hit
GTTTACTCATATCCACTGAGTTGTTGTCTTCGTTATCAGAGAATCGGAAT10.7299270072992701No Hit
GCTTTCGCGGAGAACCAGCTATTTCCGAGTTTGATTGGCCTTTCACCCCT10.7299270072992701No Hit
GTGTAAGCCTTTGGATGAAAGCTTTCATTTGGCGCTGTGGAATCACGCCG10.7299270072992701No Hit
GCTTAGTGAGGACTTGGGAGAGTACATCGGGGAGCCAGCGATGGCACCCT10.7299270072992701No Hit
AGTCTAGACAGTGGGACGGTGGCCATGGAAGTCGAAAACCGCTAAGGAGT10.7299270072992701No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)