FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s8.351000000d7c00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s8.351000000d7c00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACTTTACCGCAACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTT14.545454545454546No Hit
CCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT14.545454545454546No Hit
TCTTATGTTACAGGAGCTATCAGTCTTGAGCAGTTTAAAGCGATAGTTAA14.545454545454546No Hit
GTAAATGCTCAGTAGCTATGGTTGGAAGAAAAAATAAATGGTGAGAACAG14.545454545454546No Hit
TCCCTGAATGCGCCTTCATGCAGCGAATCGGAGTTTCAAGAGTTAAGAGG14.545454545454546No Hit
AGGTTCTCGTAGGCGTCAAAGAGGTACTGCCGCACCATGGTAGGCCCAGT14.545454545454546No Hit
AACCATCAGCATGTGCCTGTCGCATTGCATTCATCAAACGCTGAATAGCA14.545454545454546No Hit
CCAGAACGGAAAAGATCCTTCATAGAAATTTCACGCGGCGGCAAGTTGCC14.545454545454546No Hit
GTGTATTTATTAGATAAAAAACCAACGCCCTTCGGGGCTTCTTGGTGATT14.545454545454546No Hit
CTTCTCCTCATCCGACGCGTCAGTTTTTGACAGAATCGTTAGTTGATGGC14.545454545454546No Hit
GACGTGTGCTCTTCCGATCAGACGTGTGCTCTTCCGATCAGACGTGTGCT14.545454545454546TruSeq Adapter, Index 1 (95% over 24bp)
GTTCACCATAAACGTGACGATGAGGGACATAAAAAGTAAAAATGTCTACA14.545454545454546No Hit
ACACAGTCCTTGACGGTATAATAACCACCATCATGGCGACCATCCAAAGG14.545454545454546No Hit
TAACAGGGCAGAGCAACTATAAACAGAATTAGTGTCTTTTAATGAGGACC14.545454545454546No Hit
GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAAT14.545454545454546No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG14.545454545454546Illumina PCR Primer Index 10 (96% over 28bp)
GAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAACAAAG14.545454545454546No Hit
TAAGCGGCTCACCGTTAGCATCAACAGGCCACAACCAACCAGAACGTGAA14.545454545454546No Hit
ATTTGGATCGCTTTGAGGATTTCGTTGGAAACGGGATGCAATATAAAACG14.545454545454546No Hit
CTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATT14.545454545454546No Hit
GTTATATACCACCCCCAAATGGGGCAATTATTATTTACTCATTTATCACT14.545454545454546No Hit
GATACCACTGACCCTCAGCAATCTTAAACTTCTTAGACGAATCACCAGAA14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)