FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s78.351000000d84b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s78.351000000d84b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1155.00653025685677No Hit
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA341.480191554201132Illumina PCR Primer Index 5 (96% over 28bp)
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC241.0448410970831519RNA PCR Primer, Index 12 (96% over 26bp)
GAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA200.87070091423596Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGC190.827165868524162Illumina PCR Primer Index 10 (96% over 26bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC150.65302568567697RNA PCR Primer, Index 41 (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.6094906399651719No Hit
GAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAA130.5659555942533739Illumina PCR Primer Index 10 (96% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.478885502829778No Hit
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA110.478885502829778RNA PCR Primer, Index 17 (96% over 28bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.348280365694384No Hit
TCTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG60.26121027427078797TruSeq Adapter, Index 3 (96% over 28bp)
AGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG50.21767522855899Illumina PCR Primer Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT50.21767522855899No Hit
AGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA40.174140182847192Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAA40.174140182847192No Hit
TCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG40.174140182847192Illumina PCR Primer Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT40.174140182847192TruSeq Adapter, Index 6 (95% over 21bp)
TCTCCGAGCCAACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG40.174140182847192TruSeq Adapter, Index 10 (96% over 29bp)
TCTCAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG40.174140182847192Illumina PCR Primer Index 10 (96% over 29bp)
GCACCCCGGCTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA40.174140182847192No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA40.174140182847192No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAA40.174140182847192No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG30.13060513713539398TruSeq Adapter, Index 6 (96% over 28bp)
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTA30.13060513713539398No Hit
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA30.13060513713539398TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT30.13060513713539398No Hit
AGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAA30.13060513713539398RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACGATACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG30.13060513713539398Illumina PCR Primer Index 12 (96% over 29bp)
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA30.13060513713539398TruSeq Adapter, Index 12 (96% over 29bp)
TCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAA30.13060513713539398Illumina PCR Primer Index 10 (96% over 29bp)
AAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT30.13060513713539398RNA PCR Primer, Index 41 (95% over 24bp)
GATCGGAAGAGCCCACGAGACCCTAAGACAGCTCGTATGCCGTCTTCTGC30.13060513713539398Illumina PCR Primer Index 11 (95% over 23bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAA30.13060513713539398No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG30.13060513713539398No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13060513713539398No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG30.13060513713539398No Hit
CCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAA30.13060513713539398Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC30.13060513713539398No Hit
TCTCCGAGCCCACGATACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG30.13060513713539398TruSeq Adapter, Index 10 (96% over 29bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13060513713539398No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG30.13060513713539398No Hit
AAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT30.13060513713539398Illumina PCR Primer Index 10 (95% over 24bp)
CTTATACACATCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT30.13060513713539398No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCC250.004396142457.01
TTTTTTG150.00967454947.530-31
Produced by FastQC (version 0.11.2)