HG5M3BCX2_l01n01_r3zt2s76.351000000d848c.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l01n01_r3zt2s76.351000000d848c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1056
Sequences flagged as poor quality	0
Sequence length	101
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA	45	4.261363636363636	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	16	1.5151515151515151	Illumina PCR Primer Index 7 (96% over 27bp)
TCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	16	1.5151515151515151	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	14	1.3257575757575757	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	11	1.0416666666666665	RNA PCR Primer, Index 41 (96% over 31bp)
AGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA	10	0.946969696969697	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	10	0.946969696969697	Illumina PCR Primer Index 7 (96% over 27bp)
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	9	0.8522727272727272	Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCAGAGACTCGACGTCATCTCGTATGCCGTC	8	0.7575757575757576	No Hit
TCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	8	0.7575757575757576	Illumina PCR Primer Index 7 (96% over 27bp)
CATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	7	0.6628787878787878	Illumina PCR Primer Index 7 (96% over 25bp)
TCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	7	0.6628787878787878	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	7	0.6628787878787878	Illumina PCR Primer Index 7 (96% over 27bp)
CCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	7	0.6628787878787878	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	6	0.5681818181818182	Illumina PCR Primer Index 7 (96% over 27bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTCGACGT	6	0.5681818181818182	No Hit
CCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAA	6	0.5681818181818182	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	6	0.5681818181818182	RNA PCR Primer, Index 41 (96% over 31bp)
ATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTT	5	0.4734848484848485	RNA PCR Primer, Index 41 (95% over 24bp)
TCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	4	0.3787878787878788	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.3787878787878788	RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	4	0.3787878787878788	RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	4	0.3787878787878788	RNA PCR Primer, Index 41 (96% over 31bp)
GGTGAAAAGAACCCCGGGAGGGGAGTGAAAGAGCACCTGAAACCGTATGC	4	0.3787878787878788	No Hit
CATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.3787878787878788	RNA PCR Primer, Index 41 (96% over 29bp)
TTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.2840909090909091	Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGT	3	0.2840909090909091	RNA PCR Primer, Index 41 (95% over 21bp)
ATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGACATATCTCGTAT	3	0.2840909090909091	No Hit
ACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	3	0.2840909090909091	RNA PCR Primer, Index 41 (96% over 26bp)
CTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.2840909090909091	RNA PCR Primer, Index 41 (96% over 31bp)
ATACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTC	3	0.2840909090909091	Illumina PCR Primer Index 3 (95% over 21bp)
CGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAA	3	0.2840909090909091	Illumina PCR Primer Index 7 (96% over 27bp)
CATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	3	0.2840909090909091	RNA PCR Primer, Index 41 (96% over 29bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA	3	0.2840909090909091	No Hit
ATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	3	0.2840909090909091	RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCCAAGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.2840909090909091	RNA PCR Primer, Index 41 (96% over 31bp)
CTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGTCCTCCCGGCTT	3	0.2840909090909091	No Hit
CATCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	3	0.2840909090909091	Illumina PCR Primer Index 7 (96% over 25bp)
CTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.2840909090909091	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCAGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.2840909090909091	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACAAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.2840909090909091	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.2840909090909091	Illumina PCR Primer Index 3 (96% over 27bp)
TCTCCGAGCCCACGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 31bp)
CACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG	2	0.1893939393939394	Illumina PCR Primer Index 3 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTC	2	0.1893939393939394	RNA PCR Primer, Index 41 (95% over 22bp)
CTCATGGGCCGACCGCTCGGTCGGTTACAGGGCTATCACCTGCTCTGGCC	2	0.1893939393939394	No Hit
ATACACATCTCCGAGCCGACGAGACTCGACGTCATCTCGTATGCCGTCTT	2	0.1893939393939394	RNA PCR Primer, Index 41 (95% over 24bp)
CTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCTACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCTGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2	0.1893939393939394	No Hit
ATTCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGGCC	2	0.1893939393939394	No Hit
ATTACTAGCGATTCCGACTTCATGCAGGCGAGTTGCAGCCTGCAATCCGA	2	0.1893939393939394	No Hit
CTTATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	2	0.1893939393939394	RNA PCR Primer, Index 41 (95% over 21bp)
TTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGC	2	0.1893939393939394	No Hit
GCATGCTACGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACA	2	0.1893939393939394	No Hit
GTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAAGAGCA	2	0.1893939393939394	No Hit
GTAACCGACCGAGCGGTCGACCCATGAGGATCCTGAGTACGACGGCACAC	2	0.1893939393939394	No Hit
CATCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 29bp)
CACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG	2	0.1893939393939394	Illumina PCR Primer Index 7 (95% over 23bp)
CATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTC	2	0.1893939393939394	No Hit
TCTCCGAGCCCACGAAACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 31bp)
CTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 31bp)
GTTACAAACTCTCGTGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACG	2	0.1893939393939394	No Hit
TCTCCGAGCCCACTAGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTG	2	0.1893939393939394	TruSeq Adapter, Index 7 (96% over 26bp)
ACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTC	2	0.1893939393939394	RNA PCR Primer, Index 41 (95% over 22bp)
GTCTTACTTCCTATTTGGAATGGGAGATCTCATCTTAAAGGGGGCTTCAC	2	0.1893939393939394	No Hit
ATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.1893939393939394	Illumina PCR Primer Index 3 (96% over 26bp)
CTCCGAACCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 31bp)
ATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.1893939393939394	Illumina PCR Primer Index 3 (96% over 26bp)
ACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTCTG	2	0.1893939393939394	RNA PCR Primer, Index 41 (96% over 27bp)
ACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC	2	0.1893939393939394	Illumina PCR Primer Index 3 (95% over 24bp)
TCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.1893939393939394	Illumina PCR Primer Index 3 (96% over 27bp)
TTTCCAACCATTCTGAGGGAACCTTTGGGCGCCTCCGTTACACTTTGGGA	2	0.1893939393939394	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCATC	65	0.0053807776	18.269232	22-23
ACTCGAC	65	0.0053807776	18.269232	16-17
ATCTCGT	65	0.0053807776	18.269232	26-27
TCGACGT	65	0.0053807776	18.269232	18-19
TATGCCG	65	0.0053807776	18.269232	32-33
CCGTCTT	65	0.0053807776	18.269232	36-37
TCTGCTT	65	0.0053807776	18.269232	42-43
GACGTCA	65	0.0053807776	18.269232	20-21
GTCTTCT	65	0.0053807776	18.269232	38-39
CGTATGC	65	0.0053807776	18.269232	30-31
TCATCTC	65	0.0053807776	18.269232	24-25
CTCGTAT	65	0.0053807776	18.269232	28-29
CTTCTGC	65	0.0053807776	18.269232	40-41
CTTGAAA	70	0.008264681	16.964285	46-47
TGAAAAA	70	0.008264681	16.964285	48-49
AAAAAAA	400	6.4186133E-7	8.3125	52-53

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content