FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s75.351000000d8462.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s75.351000000d8462.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5644621
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT948981.681211192035745No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT714101.2650982236008406TruSeq Adapter, Index 6 (95% over 21bp)
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG564691.0004037472134975TruSeq Adapter, Index 6 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT396690.7027752616163246RNA PCR Primer, Index 6 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC175520.31095090352390353No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCT174760.30960448894620207TruSeq Adapter, Index 6 (96% over 26bp)
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGA142710.25282476892602707RNA PCR Primer, Index 6 (96% over 28bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT107340.19016334311905086No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTG100960.1788605470588725RNA PCR Primer, Index 40 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCT100810.17859480733958932No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG89590.1587174763372067No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT85240.1510110244779942No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA85140.15083386466513873No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC73110.12952153917862688No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT67740.12000805722828867No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT67120.11890966638858481No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA64800.11479955873033815No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC64420.1141263514414874No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG62820.11129179443580003No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG59280.10502033706071673No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA58920.1043825617344371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT293350.025.712964
TTCCGAT296950.025.6891572
CTTCCGA299850.025.6397131
TCCGATC306050.024.8322053
CTTGTCT44200.023.318999
TATATGC50400.021.6762923
ATGCTTG50700.021.2669666
GCTTGTC48800.020.9262268
ATCTTTT94700.020.1626837
GACTACC66950.019.9355417
TAGGACT72600.019.6932544
ATCTAGG69550.019.6880441
TATACTT14700.019.387495
TATGCTT61550.019.2929945
ATATGCT58650.019.2750974
AGGACTA71900.019.0261655
CTATACT10800.018.9117774
ATATAAG42150.018.8194332
CGATCTT132550.018.7775615
CTATAGG4101.8189894E-1218.5542491
Produced by FastQC (version 0.11.2)