FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s74.351000000d8456.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s74.351000000d8456.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253513
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT1690142.330098532945347No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT1203691.659457975742237TruSeq Adapter, Index 6 (95% over 21bp)
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG915531.2621884044324454TruSeq Adapter, Index 6 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT716020.9871354749071243RNA PCR Primer, Index 6 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCT313890.43274203823719626TruSeq Adapter, Index 6 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC274780.3788233370506126No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGA234110.32275395384277933RNA PCR Primer, Index 6 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTG178950.24670804339910882RNA PCR Primer, Index 40 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCT163880.22593190361690946No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127780.17616291581748045No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT111330.15348424963186805No Hit
CCCCAATATTTAGCCTTAGATGGCATTTACCACCCATTTTGAGCTGCATT94400.130143835132025No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT81320.11211119356924018No Hit
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC78540.10827856791598774RNA PCR Primer, Index 40 (96% over 26bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76460.10541099188765499No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG76300.10519040911624478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA285050.034.5963861
TTCCGAT285600.034.2265322
TCCGATC286850.034.0773853
CCGATCT285100.034.0533144
ATCTTTT125900.029.4260947
TCTTCCG184400.025.1914795
GATCTAG17050.025.0722836
CGATCTT177750.024.8245775
GATCTTT154500.024.502236
ACTGTAC29850.022.913633
GTGCTCT207800.022.836421
TGCTCTT221300.021.2700232
TCTTTTT173700.021.1370058
CACTGTA30300.021.0057352
CTTTTTT183350.019.7913869
CTGTACT38300.019.718514
ATCTAGG67450.019.5272391
CTAGGAC66200.019.3723143
GTATTAT13750.019.3654841
TCTAGGA69800.018.917562
Produced by FastQC (version 0.11.2)