FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s73.351000000d843c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s73.351000000d843c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4045
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA2385.88380716934487Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC862.1260815822002472Illumina PCR Primer Index 10 (96% over 25bp)
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA541.334981458590853Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA531.3102595797280594Illumina PCR Primer Index 10 (96% over 28bp)
TCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG461.1372064276885043Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG421.03831891223733TruSeq Adapter, Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGCT411.0135970333745363No Hit
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG411.0135970333745363TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG411.0135970333745363Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG380.9394313967861558Illumina PCR Primer Index 10 (96% over 28bp)
TCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG340.8405438813349814Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT280.69221260815822RNA PCR Primer, Index 29 (96% over 27bp)
CATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT260.6427688504326329Illumina PCR Primer Index 10 (96% over 26bp)
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA240.5933250927070457Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG230.5686032138442522TruSeq Adapter, Index 10 (96% over 28bp)
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA220.5438813349814586Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG210.519159456118665Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT200.4944375772558714TruSeq Adapter, Index 10 (96% over 26bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA200.4944375772558714Illumina PCR Primer Index 11 (100% over 29bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA190.4697156983930779Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA180.4449938195302843Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT170.4202719406674907Illumina PCR Primer Index 10 (96% over 26bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA170.4202719406674907Illumina PCR Primer Index 10 (96% over 28bp)
CATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT160.3955500618046972Illumina PCR Primer Index 10 (96% over 26bp)
CATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT160.3955500618046972Illumina PCR Primer Index 10 (96% over 27bp)
CTTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGT160.3955500618046972No Hit
CTTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT150.3708281829419036No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA150.3708281829419036No Hit
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA140.34610630407911RNA PCR Primer, Index 29 (96% over 28bp)
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA140.34610630407911Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA140.34610630407911RNA PCR Primer, Index 29 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT140.34610630407911No Hit
CTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA140.34610630407911RNA PCR Primer, Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.34610630407911No Hit
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA130.32138442521631644TruSeq Adapter, Index 11 (96% over 30bp)
CTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA130.32138442521631644Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA130.32138442521631644Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT120.29666254635352285Illumina PCR Primer Index 10 (96% over 26bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGC120.29666254635352285No Hit
CTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA120.29666254635352285RNA PCR Primer, Index 10 (96% over 28bp)
ATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT110.2719406674907293Illumina PCR Primer Index 10 (95% over 21bp)
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA110.2719406674907293Illumina PCR Primer Index 10 (96% over 28bp)
CTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.2472187886279357Illumina PCR Primer Index 10 (96% over 28bp)
CTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.2472187886279357Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC100.2472187886279357RNA PCR Primer, Index 11 (96% over 27bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG100.2472187886279357TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.2472187886279357Illumina PCR Primer Index 10 (96% over 28bp)
GCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA90.22249690976514214Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCT90.22249690976514214RNA PCR Primer, Index 10 (95% over 23bp)
ACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT90.22249690976514214Illumina PCR Primer Index 10 (95% over 23bp)
CACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG80.1977750309023486Illumina PCR Primer Index 10 (95% over 24bp)
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA80.1977750309023486Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT80.1977750309023486Illumina PCR Primer Index 10 (95% over 21bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCG70.173053152039555No Hit
TCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.173053152039555RNA PCR Primer, Index 29 (96% over 28bp)
CTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG70.173053152039555TruSeq Adapter, Index 10 (96% over 28bp)
GAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAAAAA70.173053152039555RNA PCR Primer, Index 29 (96% over 27bp)
ACACATCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTG60.14833127317676142Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCCACGGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG60.14833127317676142TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.14833127317676142Illumina PCR Primer Index 11 (96% over 30bp)
CACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG60.14833127317676142TruSeq Adapter, Index 10 (95% over 24bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14833127317676142No Hit
CATCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT60.14833127317676142RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACGAGAAACTCGCTAATCTCGTATGCCGTCTTCTGCTTG60.14833127317676142TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.14833127317676142RNA PCR Primer, Index 29 (96% over 28bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA60.14833127317676142RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.14833127317676142Illumina PCR Primer Index 11 (96% over 30bp)
ATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTT60.14833127317676142TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTT60.14833127317676142TruSeq Adapter, Index 10 (95% over 21bp)
CATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT60.14833127317676142TruSeq Adapter, Index 10 (96% over 26bp)
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA60.14833127317676142Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT60.14833127317676142Illumina PCR Primer Index 10 (96% over 27bp)
ACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.12360939431396785Illumina PCR Primer Index 10 (95% over 23bp)
ACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.12360939431396785RNA PCR Primer, Index 10 (95% over 23bp)
CATCTCCGAGCCGACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT50.12360939431396785TruSeq Adapter, Index 10 (96% over 26bp)
TCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAA50.12360939431396785RNA PCR Primer, Index 29 (96% over 27bp)
CTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA50.12360939431396785Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.12360939431396785Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCT50.12360939431396785TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGAACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.12360939431396785Illumina PCR Primer Index 10 (96% over 28bp)
AGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG50.12360939431396785Illumina PCR Primer Index 10 (95% over 24bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.12360939431396785No Hit
TCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG50.12360939431396785TruSeq Adapter, Index 11 (96% over 29bp)
TCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG50.12360939431396785TruSeq Adapter, Index 10 (96% over 28bp)
AAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT50.12360939431396785No Hit
TCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.12360939431396785Illumina PCR Primer Index 10 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCCC201.5402386E-594.987512
CGTCTCC254.3729786E-576.95191
TCCGATC200.001926780471.240633
CTTATAC405.8050464E-660.1186751
TTATACA406.270071E-659.367192
GATCTTT300.00912542248.094941
ATCTTTT300.00959965147.4937552
TATACAC554.1325664E-543.1761363
TCTCCGA1351.8189894E-1239.188471
TACACAT651.1073353E-436.5336535
ATACACA651.1073353E-436.5336534
CTCCGAG1351.5643309E-1035.1805532
CGAGCCC851.2653065E-533.5255
CACATCT752.5699387E-431.66257
CCGAGCC1251.3900717E-730.3959984
CATCTCC803.478543E-430.0593381
ACACATC803.753819E-429.6835966
TCCGAGC1451.3862518E-829.4788783
GGAAGAG756.8511115E-418.997518-19
AAGAGCC800.001057623117.81015820-21
Produced by FastQC (version 0.11.2)