FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s72.351000000d842f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s72.351000000d842f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGCATTCATCAAACGCTGAATAGCAAAGCCTCTACGCGATTTCATA14.0No Hit
CAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT14.0No Hit
ATTAGTGGTTGAACAGCATCGGACTCAGATAGTAATCCACGCTCTTTTAA14.0No Hit
CTTTGAGCGTATCGAGGCTCCTAAACCTGCTATTGAGGCTTGTGGCATTT14.0No Hit
ACGACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTA14.0No Hit
ACCATGAAAAATATCAACCACACCAGAAGCAGCATCAGTGACGACATTAG14.0No Hit
GCAGGAGAAACATACGAAGGCGCATAACGATACCACTGACCCTCAGCAAT14.0No Hit
CTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATCC14.0No Hit
GACAAATAATCTCTTTAATAACCTGATTCAGCGAAACCAATCCGCGGCAT14.0No Hit
ACGCTTGCCTTTACTACCTCGCAACGGCTGCGGACGACCAGGGCGAGCGC14.0No Hit
GCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGA14.0No Hit
GAAGCAATACCGCGAGCAATAGCACCAAACATAAATCACCTCACTTAAGT14.0No Hit
TCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGA14.0No Hit
AAGCCAAGCATTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCTCAAT14.0No Hit
ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGAT14.0No Hit
GAACGAACCATAAAAAAGCCTCCAAGATTTGGAGGCATGAAAACATACAA14.0No Hit
ATGGTTGAGGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACT14.0No Hit
GAAGTAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGCTCCT14.0No Hit
GTCCGCATAAAGTGCACCGCATGGAAATGAAGACGGCCATTAGCTGTACC14.0No Hit
CAACTGGACAATCCGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAA14.0No Hit
ACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTA14.0No Hit
GCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGC14.0No Hit
AAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGA14.0No Hit
TTTAATACCAGCATCACCCATGCCTACAGTATTGTTATCGGTAGCAAGCA14.0No Hit
TTTCTGTTCTCCATGAGTCCCCCTTAGGACACCTGCGTTATTGTTTTACA14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers