FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s71.351000000d83f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s71.351000000d83f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422088
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT1032591.9044139453288107No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT728731.3440025318659528RNA PCR Primer, Index 41 (95% over 22bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG440840.8130447163528146RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT405850.748512381208125RNA PCR Primer, Index 41 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC225950.41672138113582813No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT171560.31640947177544887RNA PCR Primer, Index 41 (96% over 27bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT133600.24639954202145004No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT116580.2150094207250048RNA PCR Primer, Index 41 (95% over 21bp)
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA101190.18662552138585725RNA PCR Primer, Index 41 (96% over 29bp)
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT91540.16882794967547557No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG90920.1676844787469329No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG87740.16181957946827863TruSeq Adapter, Index 3 (95% over 24bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA84330.15553048936129404No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79790.14715733127164293No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT79400.14643805117143063No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77830.14354248769108874No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC77670.14324739841920678No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA77560.14304452454478792No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC76960.1419379397752305No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT75560.1393559086462632No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71120.13116718135153838No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG70620.1302450273769072No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA66530.12270180786442418No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG63930.11790660719634208No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA63690.1174639732885191No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA63080.11633894543946909No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA60670.11189416328174681No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56590.10436938684875642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA229300.046.8382231
TTCCGAT229050.046.6819272
CCGATCT231600.045.634684
TCCGATC235750.045.274633
TCTTCCG123450.038.1701745
GCTCTTC124650.038.0313573
GTGCTCT136900.035.353511
CTCTTCC137400.034.4676784
TGCTCTT149700.031.9212382
CGATCTC59800.030.3435045
ATCTTTT100800.030.2517197
ATATAAG27100.027.34382
GATCTAG19050.025.9323526
TATGCTT39400.024.5944715
CGATCTT161950.024.4911445
TCTTTTT127050.024.001368
CTTGTCT40600.023.8660039
CGATCTG145800.023.8157045
CTTTTTT129850.023.4106569
GATCTTT139700.022.8154836
Produced by FastQC (version 0.11.2)