FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s70.351000000d83ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s70.351000000d83ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4498803
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT817671.8175279068676713No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT565211.2563564130280878RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT284550.6325015787532817RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG253310.5630608853066026RNA PCR Primer, Index 41 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC173920.3865917222870172No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156740.348403786518325No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121560.27020520791863967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113570.25244492812866004No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110100.2447317653162408No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT90960.20218711510595153RNA PCR Primer, Index 41 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT88110.19585209665771097RNA PCR Primer, Index 41 (95% over 21bp)
GAATAATAAAATAGGACGTGCGGTTCTATTTTGTTGGTTTTTGGAATCGC61210.13605841376028244No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA58390.1297900797167602RNA PCR Primer, Index 41 (96% over 29bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG54060.12016529730241576TruSeq Adapter, Index 3 (95% over 24bp)
GATTAATAGGGACAGTCGGGGGCATTAGTACTCACATGCTAGAGGTGAAA52560.1168310770664997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT258400.044.097544
CTTCCGA263450.043.7223661
TCCGATC263700.043.3193173
TTCCGAT266500.043.1315232
TGCTCTT139350.041.652482
CGATCTG72650.041.6466875
GTGCTCT142800.041.047181
GCTCTTC145050.039.950183
GATCTAG11950.039.3499266
GATCTTT121500.038.8585666
CGATCTT142100.038.172335
TCTTCCG154550.037.0334935
CTCTTCC168000.034.3513344
CGATCTA37700.033.3872875
CGATCTC55050.032.183125
ATCTTTT156700.029.7655757
GATCTGG38350.028.858066
CTTTTTT174000.026.7788289
ACTTTAG14150.026.2051491
TCTTTTT186600.025.0724288
Produced by FastQC (version 0.11.2)