HG5M3BCX2_l01n01_r3zt2s7.351000000d7be5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l01n01_r3zt2s7.351000000d7be5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1361
Sequences flagged as poor quality	0
Sequence length	101
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	44	3.2329169728141074	RNA PCR Primer, Index 47 (96% over 29bp)
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA	31	2.2777369581190303	RNA PCR Primer, Index 47 (96% over 29bp)
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA	23	1.6899338721528288	Illumina PCR Primer Index 9 (100% over 27bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA	21	1.5429831006612784	RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	16	1.1756061719324025	No Hit
TCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAA	16	1.1756061719324025	TruSeq Adapter, Index 9 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC	13	0.9551800146950772	RNA PCR Primer, Index 41 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	9	0.6612784717119765	Illumina PCR Primer Index 10 (95% over 21bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACATGCGCA	9	0.6612784717119765	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9	0.6612784717119765	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	8	0.5878030859662012	No Hit
TCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	8	0.5878030859662012	TruSeq Adapter, Index 9 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	8	0.5878030859662012	No Hit
ATACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	7	0.5143277002204262	TruSeq Adapter, Index 9 (95% over 21bp)
TCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG	7	0.5143277002204262	Illumina PCR Primer Index 3 (96% over 28bp)
TCTCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA	7	0.5143277002204262	RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCACTAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG	7	0.5143277002204262	RNA PCR Primer, Index 41 (96% over 29bp)
CTTATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGT	6	0.440852314474651	No Hit
CCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	6	0.440852314474651	RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG	6	0.440852314474651	Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	6	0.440852314474651	RNA PCR Primer, Index 47 (96% over 29bp)
CTTATACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	5	0.36737692872887584	No Hit
CATCTCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT	5	0.36737692872887584	RNA PCR Primer, Index 47 (96% over 28bp)
CTTATACACATCTCCGAGCACACGAGACATGCGCAGATCTCGTATGCCGT	5	0.36737692872887584	No Hit
TCTCCGAGCCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT	5	0.36737692872887584	RNA PCR Primer, Index 47 (96% over 28bp)
ATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	5	0.36737692872887584	TruSeq Adapter, Index 9 (95% over 21bp)
TCTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	5	0.36737692872887584	RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCACGAGACATGCGCAGACCTCGTATGCCGTCTTCTGCTTG	5	0.36737692872887584	Illumina PCR Primer Index 9 (96% over 27bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACATGCGC	4	0.2939015429831006	No Hit
CCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAA	4	0.2939015429831006	RNA PCR Primer, Index 47 (96% over 29bp)
ACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCT	4	0.2939015429831006	Illumina PCR Primer Index 9 (95% over 23bp)
GATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC	4	0.2939015429831006	RNA PCR Primer, Index 47 (96% over 26bp)
TCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	4	0.2939015429831006	Illumina PCR Primer Index 9 (96% over 28bp)
ATACACATCTCCGAGCACACGAGACATGCGCAGATCTCGTATGCCGTCTT	4	0.2939015429831006	Illumina PCR Primer Index 9 (95% over 21bp)
TCTCCGAGCCCACGATACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	4	0.2939015429831006	Illumina PCR Primer Index 9 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT	4	0.2939015429831006	Illumina PCR Primer Index 10 (95% over 23bp)
ATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTT	4	0.2939015429831006	Illumina PCR Primer Index 9 (95% over 21bp)
TATCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	4	0.2939015429831006	RNA PCR Primer, Index 47 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	3	0.2204261572373255	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT	3	0.2204261572373255	No Hit
CTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA	3	0.2204261572373255	Illumina Paired End PCR Primer 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	3	0.2204261572373255	RNA PCR Primer, Index 47 (95% over 22bp)
TCTACGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG	3	0.2204261572373255	TruSeq Adapter, Index 3 (96% over 28bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.2204261572373255	No Hit
TCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG	3	0.2204261572373255	TruSeq Adapter, Index 9 (96% over 28bp)
TCTCCGAGCCCACGATACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG	3	0.2204261572373255	Illumina PCR Primer Index 3 (96% over 28bp)
TCTCCGAGCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA	3	0.2204261572373255	RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA	3	0.2204261572373255	RNA PCR Primer, Index 41 (96% over 29bp)
TCTCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA	3	0.2204261572373255	RNA PCR Primer, Index 41 (96% over 29bp)
AGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA	3	0.2204261572373255	RNA PCR Primer, Index 47 (96% over 29bp)
CTTATACACATCTGCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	2	0.1469507714915503	No Hit
TCTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG	2	0.1469507714915503	TruSeq Adapter, Index 3 (96% over 28bp)
TTATACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	2	0.1469507714915503	No Hit
TTATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTC	2	0.1469507714915503	No Hit
ACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT	2	0.1469507714915503	Illumina Paired End PCR Primer 2 (95% over 22bp)
GGATTGAGGGAGGCCAGCCCCTTTCCGGCGAGTTGCGCATTTATCCGGCT	2	0.1469507714915503	No Hit
TATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCT	2	0.1469507714915503	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT	2	0.1469507714915503	Illumina PCR Primer Index 10 (96% over 26bp)
GCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	2	0.1469507714915503	Illumina PCR Primer Index 9 (100% over 27bp)
ACACATCTCCGAGTCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT	2	0.1469507714915503	RNA PCR Primer, Index 47 (95% over 24bp)
TCTCCAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA	2	0.1469507714915503	RNA PCR Primer, Index 41 (96% over 29bp)
CCCCGACGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC	2	0.1469507714915503	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTGATCTCGTATGCCGTCTTCT	2	0.1469507714915503	Illumina PCR Primer Index 10 (95% over 23bp)
CCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2	0.1469507714915503	Illumina Paired End PCR Primer 2 (96% over 27bp)
AGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAA	2	0.1469507714915503	RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCCGCTTG	2	0.1469507714915503	Illumina PCR Primer Index 9 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGTGCGCAGATCTCGTATGCCGTCTT	2	0.1469507714915503	Illumina PCR Primer Index 9 (95% over 21bp)
ACACATCTCCGAGCCGACGAGACCCTAAGACATCTCGTATGCCGTCTTCT	2	0.1469507714915503	Illumina PCR Primer Index 3 (95% over 23bp)
TCTCCGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG	2	0.1469507714915503	Illumina PCR Primer Index 3 (96% over 28bp)
CATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT	2	0.1469507714915503	Illumina PCR Primer Index 9 (96% over 26bp)
CTTATACACATCTCTGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	2	0.1469507714915503	No Hit
CTCCGAGCCCACGAGACATGCGCAGAGCTCGTATGCCGTCTTCTGCTTGA	2	0.1469507714915503	Illumina PCR Primer Index 9 (96% over 27bp)
AAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	2	0.1469507714915503	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCAAATCTCGTATGCCGTCTT	2	0.1469507714915503	RNA PCR Primer, Index 13 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT	2	0.1469507714915503	RNA PCR Primer, Index 47 (95% over 24bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT	2	0.1469507714915503	RNA PCR Primer, Index 47 (96% over 27bp)
ATACACATCTCCGAGCCAACGAGACATGCGCAGAACTCGTATGCCGTCTT	2	0.1469507714915503	No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1469507714915503	No Hit
TCTCCGAGCCCACGAACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA	2	0.1469507714915503	RNA PCR Primer, Index 47 (96% over 29bp)
ACACATCTCCGACCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG	2	0.1469507714915503	Illumina PCR Primer Index 9 (95% over 24bp)
GCACCCCTGGTGAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTA	2	0.1469507714915503	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1469507714915503	No Hit
CTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA	2	0.1469507714915503	RNA PCR Primer, Index 47 (96% over 29bp)
CATCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT	2	0.1469507714915503	Illumina PCR Primer Index 3 (96% over 26bp)
TCTCCGAAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT	2	0.1469507714915503	RNA PCR Primer, Index 47 (96% over 28bp)
CACATCTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG	2	0.1469507714915503	RNA PCR Primer, Index 41 (96% over 25bp)
CTTATACACATCTTCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	2	0.1469507714915503	No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA	2	0.1469507714915503	No Hit
CTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA	2	0.1469507714915503	RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGCATGCGCAGATCTCGTATGCCGTCTTC	2	0.1469507714915503	RNA PCR Primer, Index 47 (95% over 23bp)
TCTCCGAGCCCACGAGGACCCTAAGACATCTCGTATGCCGTCTTCTGCTT	2	0.1469507714915503	RNA PCR Primer, Index 17 (96% over 27bp)
GTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGTTTC	2	0.1469507714915503	No Hit
ACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT	2	0.1469507714915503	Illumina Paired End PCR Primer 2 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACGCGCTGATCTCGTATGCCGT	2	0.1469507714915503	No Hit
CATATCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT	2	0.1469507714915503	RNA PCR Primer, Index 47 (96% over 27bp)
AAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT	2	0.1469507714915503	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCCGA	40	3.6865685E-4	47.499996	1
CGAGCCC	50	0.0011090648	38.0	5
CCGAGCC	50	0.0011090648	38.0	4
TCCGAGC	50	0.0011090648	38.0	3
CTCCGAG	55	0.0017734222	34.545452	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content