FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s69.351000000d83c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s69.351000000d83c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4606
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA671.4546244029526705Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC631.3677811550151975RNA PCR Primer, Index 29 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA481.0421189752496742No Hit
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA460.998697351280938RNA PCR Primer, Index 42 (96% over 28bp)
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG430.9335649153278333TruSeq Adapter, Index 7 (96% over 28bp)
GAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAA410.8901432913590968RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT300.6513243595310464No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA210.4559270516717325RNA PCR Primer, Index 7 (96% over 28bp)
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA200.43421623968736434Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAA200.43421623968736434TruSeq Adapter, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG190.4125054277029961RNA PCR Primer, Index 29 (96% over 30bp)
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC190.4125054277029961Illumina Paired End PCR Primer 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT170.36908380373425964RNA PCR Primer, Index 41 (95% over 22bp)
TCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA170.36908380373425964Illumina Paired End PCR Primer 2 (96% over 29bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA160.34737299174989145Illumina PCR Primer Index 10 (96% over 29bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA140.303951367781155RNA PCR Primer, Index 29 (96% over 30bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG130.2822405557967868No Hit
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA130.2822405557967868Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG120.26052974381241856RNA PCR Primer, Index 41 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.23881893182805036No Hit
TCTCCGAGCCAACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG110.23881893182805036Illumina Paired End PCR Primer 2 (96% over 29bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA110.23881893182805036RNA PCR Primer, Index 29 (96% over 30bp)
AGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA100.21710811984368217TruSeq Adapter, Index 7 (96% over 28bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA100.21710811984368217No Hit
TCTCCTAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG100.21710811984368217Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG100.21710811984368217TruSeq Adapter, Index 10 (96% over 29bp)
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG100.21710811984368217Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGC90.19539730785931392RNA PCR Primer, Index 7 (96% over 25bp)
TCTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG90.19539730785931392RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT90.19539730785931392No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT90.19539730785931392RNA PCR Primer, Index 41 (95% over 24bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.17368649587494572No Hit
CCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAA80.17368649587494572RNA PCR Primer, Index 7 (96% over 28bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.17368649587494572No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG80.17368649587494572TruSeq Adapter, Index 5 (96% over 28bp)
TCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG70.1519756838905775Illumina PCR Primer Index 7 (96% over 28bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.1519756838905775TruSeq Adapter, Index 10 (96% over 29bp)
TCTCCTAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG70.1519756838905775TruSeq Adapter, Index 7 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTGCAGCT70.1519756838905775No Hit
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.1519756838905775RNA PCR Primer, Index 29 (96% over 30bp)
CCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAA70.1519756838905775RNA PCR Primer, Index 5 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT70.1519756838905775TruSeq Adapter, Index 10 (95% over 22bp)
TCTCCGAGCCCACGATACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG70.1519756838905775Illumina PCR Primer Index 7 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTGAGC70.1519756838905775No Hit
AGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAA70.1519756838905775Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.1519756838905775TruSeq Adapter, Index 10 (96% over 29bp)
TCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG70.1519756838905775TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT60.13026487190620928No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC60.13026487190620928No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTAGCGCT60.13026487190620928No Hit
CCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAA60.13026487190620928RNA PCR Primer, Index 29 (96% over 30bp)
GCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA60.13026487190620928Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC60.13026487190620928Illumina PCR Primer Index 10 (96% over 25bp)
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT60.13026487190620928Illumina PCR Primer Index 10 (95% over 22bp)
GAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA60.13026487190620928TruSeq Adapter, Index 5 (96% over 28bp)
ATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT50.10855405992184108Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT50.10855405992184108TruSeq Adapter, Index 7 (95% over 21bp)
CGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA50.10855405992184108RNA PCR Primer, Index 29 (96% over 30bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT50.10855405992184108No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA50.10855405992184108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATCG200.001944816471.259
ACATCTA250.004710035457.0000045
TTCCGAT352.505594E-454.285712
CCGATCT352.505594E-454.285714
CTTCCGA352.505594E-454.285711
CGATCTT300.00968848347.55
TCCGATC404.8447528E-447.4999963
TACACAT1104.784488E-834.5454562
ATACACA1157.077506E-833.0434761
CACATCT1201.0292388E-731.6666684
ACACATC1302.0794323E-729.2307663
CTTATAC650.005266868529.2307661
TTATACA700.007566242527.1428552
ATCTCCG907.6600467E-426.3888917
CATCTCC950.001050655725.0000026
TCTCCGA950.001050655725.0000028
CTCCGAG1000.001417299323.7500029
ATCGGAA852.6253692E-622.3529412-13
ACATCTC1302.3721413E-421.9230755
CGGAAGA904.3130804E-621.11111314-15
Produced by FastQC (version 0.11.2)