HG5M3BCX2_l01n01_r3zt2s68.351000000d83a7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l01n01_r3zt2s68.351000000d83a7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569
Sequences flagged as poor quality	0
Sequence length	101
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA	48	3.0592734225621414	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	44	2.804333970681963	Illumina PCR Primer Index 7 (96% over 27bp)
TCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	41	2.613129381771829	RNA PCR Primer, Index 41 (96% over 31bp)
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	32	2.0395156150414278	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	13	0.82855321861058	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	12	0.7648183556405354	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	12	0.7648183556405354	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	11	0.7010834926704907	RNA PCR Primer, Index 41 (96% over 31bp)
CCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAA	9	0.5736137667304015	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	9	0.5736137667304015	Illumina PCR Primer Index 3 (96% over 26bp)
CTTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGT	8	0.5098789037603569	RNA PCR Primer, Index 41 (95% over 21bp)
TCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	8	0.5098789037603569	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	8	0.5098789037603569	RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	8	0.5098789037603569	RNA PCR Primer, Index 41 (95% over 24bp)
ATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTT	8	0.5098789037603569	RNA PCR Primer, Index 41 (95% over 24bp)
CTTATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	8	0.5098789037603569	RNA PCR Primer, Index 41 (95% over 21bp)
TCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	8	0.5098789037603569	RNA PCR Primer, Index 41 (96% over 31bp)
AGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA	7	0.44614404079031234	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	7	0.44614404079031234	RNA PCR Primer, Index 41 (96% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.44614404079031234	No Hit
CTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	7	0.44614404079031234	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	6	0.3824091778202677	RNA PCR Primer, Index 41 (95% over 21bp)
ACACATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	5	0.3186743148502231	RNA PCR Primer, Index 41 (96% over 26bp)
ACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	5	0.3186743148502231	RNA PCR Primer, Index 41 (96% over 26bp)
TCTCCGAGCCCACGGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	5	0.3186743148502231	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCT	5	0.3186743148502231	RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	5	0.3186743148502231	RNA PCR Primer, Index 41 (96% over 26bp)
TCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTG	5	0.3186743148502231	TruSeq Adapter, Index 7 (96% over 26bp)
TCTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTG	5	0.3186743148502231	TruSeq Adapter, Index 7 (96% over 26bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTCGACGT	4	0.25493945188017847	No Hit
TTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTC	4	0.25493945188017847	No Hit
TCTCCAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.25493945188017847	Illumina PCR Primer Index 7 (96% over 27bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA	4	0.25493945188017847	No Hit
ACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	4	0.25493945188017847	RNA PCR Primer, Index 41 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTC	4	0.25493945188017847	RNA PCR Primer, Index 41 (95% over 22bp)
ATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTC	4	0.25493945188017847	Illumina PCR Primer Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTCTG	4	0.25493945188017847	RNA PCR Primer, Index 41 (96% over 27bp)
CATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.25493945188017847	RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.25493945188017847	Illumina PCR Primer Index 3 (96% over 27bp)
TCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.25493945188017847	Illumina PCR Primer Index 3 (96% over 27bp)
CACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG	3	0.19120458891013384	Illumina PCR Primer Index 3 (95% over 23bp)
CTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.19120458891013384	RNA PCR Primer, Index 41 (96% over 31bp)
CTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.19120458891013384	RNA PCR Primer, Index 41 (96% over 31bp)
GTACAACGAGTGGCGAGACCGCGAGGTTTAGCTAATCTCCCAAAGCCATT	3	0.19120458891013384	No Hit
ATTCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGGCC	3	0.19120458891013384	No Hit
CGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAA	3	0.19120458891013384	Illumina PCR Primer Index 7 (96% over 27bp)
CACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG	3	0.19120458891013384	Illumina PCR Primer Index 7 (95% over 23bp)
GTGTAGCCCAGCTCATAAGGGGCATGATGATTTGACGTCATCCCCGCCTT	3	0.19120458891013384	No Hit
GTACTGGTTCACTATCGGTCACTAGGTAGTATTTAGGCTTGGGAGATGGT	3	0.19120458891013384	No Hit
ATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	3	0.19120458891013384	RNA PCR Primer, Index 41 (95% over 24bp)
CTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.19120458891013384	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	3	0.19120458891013384	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTCTTCTG	3	0.19120458891013384	RNA PCR Primer, Index 41 (96% over 27bp)
TCTCCGAGCCCACGAGACTCGACGTCAACTCGTATGCCGTCTTCTGCTTG	3	0.19120458891013384	TruSeq Adapter, Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTC	3	0.19120458891013384	RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCGAGACTCGACGTCATCTCGTATGCCGTCT	3	0.19120458891013384	RNA PCR Primer, Index 41 (95% over 23bp)
CTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTGA	3	0.19120458891013384	RNA PCR Primer, Index 7 (96% over 26bp)
GCTTAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACG	3	0.19120458891013384	No Hit
TCTCCGAGCCCACGAGGCTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.19120458891013384	Illumina PCR Primer Index 7 (96% over 27bp)
GCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3	0.19120458891013384	RNA PCR Primer, Index 41 (96% over 31bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	3	0.19120458891013384	RNA PCR Primer, Index 41 (95% over 22bp)
ATACACATCTCCGAGCCCAAGAGACTCGACGTCATCTCGTATGCCGTCTT	3	0.19120458891013384	RNA PCR Primer, Index 41 (95% over 24bp)
GAGCTATCCTTAGTACGAGAGGACCGGGATGGACGCACCGCTGGTGCACC	3	0.19120458891013384	No Hit
GATCGGAAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC	3	0.19120458891013384	Illumina PCR Primer Index 7 (95% over 24bp)
TATCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.19120458891013384	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.19120458891013384	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCATGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.19120458891013384	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTC	2	0.12746972594008923	RNA PCR Primer, Index 41 (95% over 22bp)
GAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTGAAAAA	2	0.12746972594008923	RNA PCR Primer, Index 45 (96% over 26bp)
TCTCCGACCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.12746972594008923	Illumina PCR Primer Index 3 (96% over 27bp)
TCTCCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 30bp)
GGCTCGGAGATGTGTATAAGAGACCTCCGAGCCCACGAGACTCGACGTCA	2	0.12746972594008923	No Hit
AAGTAACTGTGCGCGTCTTGACGGTACCTAATCAGAAAGCCACGGCTAAC	2	0.12746972594008923	No Hit
TCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAG	2	0.12746972594008923	No Hit
ACATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGC	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 28bp)
CATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	2	0.12746972594008923	No Hit
CTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 31bp)
ATACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTC	2	0.12746972594008923	Illumina PCR Primer Index 3 (95% over 21bp)
GTTACAGGGCTATCACCTGCTCTGGCCACCCTTCCCAGGGTGTTCACCTT	2	0.12746972594008923	No Hit
CTCCCAAAGCCATTCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATG	2	0.12746972594008923	No Hit
ACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC	2	0.12746972594008923	Illumina PCR Primer Index 7 (95% over 24bp)
GGGTTAAGTCCCGTAACGAGCGCAACCCTTATTGTTAGTTGCCAGCATTG	2	0.12746972594008923	No Hit
GTTTAAGGTGAACACCCTGGGAAGGGTGGCCAGAGCAGGTGATAGCCCTG	2	0.12746972594008923	No Hit
CCGTAGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCG	2	0.12746972594008923	No Hit
CACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTGC	2	0.12746972594008923	Illumina PCR Primer Index 3 (95% over 24bp)
CTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA	2	0.12746972594008923	No Hit
GCTTCGCCTAAGCTAACACGTCCACTTAACCTTCCAGCACCGGGCAGGTG	2	0.12746972594008923	No Hit
TCTCCGAGCCCACGAGATCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 31bp)
TCCGAGCCCACGAGACTCGACGTCAACTCGTATGCCGTCTTCTGCTTGAA	2	0.12746972594008923	TruSeq Adapter, Index 7 (96% over 26bp)
GTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACT	2	0.12746972594008923	No Hit
GTAAAACTCTGTTATTAGGGAAGAACAAATGCGTAAGTAACTGTGCGCGT	2	0.12746972594008923	No Hit
GTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGT	2	0.12746972594008923	No Hit
CATCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.12746972594008923	Illumina PCR Primer Index 7 (96% over 26bp)
CATCTGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 31bp)
TTATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	2	0.12746972594008923	No Hit
CTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.12746972594008923	Illumina PCR Primer Index 7 (96% over 27bp)
CATCTCCGAGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.12746972594008923	Illumina PCR Primer Index 3 (96% over 26bp)
ATACACATCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTCTTC	2	0.12746972594008923	Illumina PCR Primer Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCT	2	0.12746972594008923	Illumina PCR Primer Index 7 (95% over 22bp)
ACCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGGGCTTCATTTCA	2	0.12746972594008923	No Hit
ACACATCTCCGAGCCCAGAGACTCGACGTCATCTCGTATGCCGTCTTCTG	2	0.12746972594008923	Illumina PCR Primer Index 7 (95% over 23bp)
TCTCCGAGCCCAAGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 31bp)
GTCCCTATCCGTCGCGGGCGTTGGAGATTTGAAGAGAGCTATCCTTAGTA	2	0.12746972594008923	No Hit
GTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATGATTTG	2	0.12746972594008923	No Hit
CTCCGAGCCCACGGGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTGA	2	0.12746972594008923	Illumina PCR Primer Index 7 (96% over 26bp)
CATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.12746972594008923	Illumina PCR Primer Index 7 (96% over 25bp)
TCTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGCCTTCTGCTTG	2	0.12746972594008923	TruSeq Adapter, Index 7 (96% over 26bp)
CCCTGTAACCGACCGAGCGGTCGGCCCATGAGGATCCTGAGTACGGCGGC	2	0.12746972594008923	No Hit
ATACACATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTT	2	0.12746972594008923	RNA PCR Primer, Index 41 (95% over 24bp)
GTAGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAG	2	0.12746972594008923	No Hit
CATCTCCGGGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACGAGAATCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 31bp)
GGACAATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAG	2	0.12746972594008923	No Hit
CTCCGAGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	2	0.12746972594008923	RNA PCR Primer, Index 41 (96% over 31bp)
GTTATCCCCAGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGG	2	0.12746972594008923	No Hit
ACACATCTCCGAGCCCACGGACTCGACGTCATCTCGTATGCCGTCTTCTG	2	0.12746972594008923	Illumina PCR Primer Index 7 (95% over 23bp)
ATTCTAAGGTGAGCGAGCGAACTCTCGTTAAGGAACTCGGCAAAATGACC	2	0.12746972594008923	No Hit
TATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGACATATCTCGTA	2	0.12746972594008923	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCCGA	50	1.7192086E-5	47.5	1
CTCCGAG	70	1.2572881E-4	33.92857	2
CGAGCCC	75	1.8881974E-4	31.666668	5
CCGAGCC	75	1.8881974E-4	31.666668	4
AGCCCAC	80	2.7609136E-4	29.687498	7
GAGCCCA	80	2.7609136E-4	29.687498	6
TCCGAGC	80	2.7609136E-4	29.687498	3
GAAAAAA	70	3.28424E-7	27.142855	48-49
CCGTCTT	70	3.28424E-7	27.142855	36-37
GTCTTCT	70	3.28424E-7	27.142855	38-39
GCCGTCT	70	3.28424E-7	27.142855	34-35
TCTTCTG	70	3.28424E-7	27.142855	38-39
CGTCTTC	70	3.28424E-7	27.142855	36-37
CTTCTGC	70	3.28424E-7	27.142855	40-41
TTCTGCT	65	6.4604483E-6	25.576923	40-41
TCTGCTT	65	6.4604483E-6	25.576923	42-43
GCTTGAA	65	6.4604483E-6	25.576923	44-45
CTTGAAA	65	6.4604483E-6	25.576923	46-47
TGCCGTC	75	6.012506E-7	25.333334	34-35
ATGCCGT	75	6.012506E-7	25.333334	32-33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content