FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s67.351000000d838d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s67.351000000d838d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225491
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT1267202.4250352741972No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT935311.7898987865446518RNA PCR Primer, Index 41 (95% over 22bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG765711.465335984695027RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT514930.9854193605921433RNA PCR Primer, Index 41 (95% over 24bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA248650.4758404521221068No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT236020.451670474602291RNA PCR Primer, Index 41 (96% over 27bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT216780.4148509680717085No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC216260.4138558462735847No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA191580.36662583477801414RNA PCR Primer, Index 41 (96% over 29bp)
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA165520.3167549231258842No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT157940.3022491092224635No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG127780.24453204493128014TruSeq Adapter, Index 3 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT125600.24036018816222243RNA PCR Primer, Index 41 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77930.14913431101498403No Hit
GTCTTAGACTTTTGCGGTTGTTGATTTTGCTTTCTTGTTTATTTAGCTGC75000.14352718242170928No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT68950.1319493230396914No Hit
GATATAACTTTGGTCTGATATTTGTTTTTTCTCATTTTGTCATTATCATT64790.12398834865470058No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59000.11290805017174461No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC57110.10929116517471756RNA PCR Primer, Index 17 (96% over 25bp)
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTACGATTGCAAAACTTTCG56860.10881274123331186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA220200.029.0419711
TTCCGAT221450.028.7005312
CCGATCT225000.027.8676874
TCCGATC226950.027.7747553
ATCTTTT104900.024.7680767
CGATCTT122300.024.1198525
GATCTTT104250.024.0128696
ATAACTT39400.022.7863664
TAGGACT44450.022.1211684
TTTGGTC37150.022.1191129
GATATAA51600.021.8383481
CTAGGAC43350.021.2579863
TCTTCCG161900.021.2422855
ATCTAGG43700.020.8901181
TATAACT42950.020.7923773
TATGCTT34200.020.2785195
AACTTTG41600.019.9827026
GTCTTAG48100.019.6711221
GCTCTTC174400.019.6652833
TCTAGGA47900.019.6353742
Produced by FastQC (version 0.11.2)