FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s65.351000000d8357.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s65.351000000d8357.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5628
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA3015.348258706467662Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC1121.9900497512437811Illumina PCR Primer Index 10 (96% over 25bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA911.616915422885572Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG671.1904761904761905Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG591.0483297796730633TruSeq Adapter, Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGCT591.0483297796730633No Hit
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA571.0127931769722816Illumina PCR Primer Index 10 (96% over 28bp)
TCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG550.9772565742714996Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG520.9239516702203269Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG500.8884150675195452TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG460.8173418621179815Illumina PCR Primer Index 10 (96% over 28bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA380.6751954513148543Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA370.6574271499644634Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.6041222459132907No Hit
TCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG330.5863539445628998Illumina PCR Primer Index 10 (96% over 28bp)
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA300.5330490405117271Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT300.5330490405117271Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA280.4975124378109453Illumina PCR Primer Index 10 (96% over 28bp)
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA280.4975124378109453Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT270.4797441364605544No Hit
ACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT260.46197583511016344Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT260.46197583511016344Illumina PCR Primer Index 10 (96% over 27bp)
TCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG260.46197583511016344TruSeq Adapter, Index 10 (96% over 28bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA260.46197583511016344Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA230.40867093105899077RNA PCR Primer, Index 29 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGT220.39090262970859985No Hit
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA200.35536602700781805Illumina PCR Primer Index 11 (100% over 29bp)
CTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA200.35536602700781805Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT190.33759772565742713Illumina PCR Primer Index 10 (96% over 26bp)
CTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA190.33759772565742713Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCT180.31982942430703626RNA PCR Primer, Index 10 (95% over 23bp)
CATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT170.30206112295664533Illumina PCR Primer Index 10 (96% over 26bp)
CTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA160.28429282160625446Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT160.28429282160625446TruSeq Adapter, Index 10 (96% over 26bp)
ATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT160.28429282160625446Illumina PCR Primer Index 10 (95% over 21bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA150.26652452025586354TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTT150.26652452025586354TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT150.26652452025586354No Hit
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA150.26652452025586354Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT140.24875621890547264Illumina PCR Primer Index 10 (95% over 21bp)
CTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA140.24875621890547264RNA PCR Primer, Index 10 (96% over 28bp)
CATCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT140.24875621890547264Illumina PCR Primer Index 10 (96% over 26bp)
CTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG140.24875621890547264TruSeq Adapter, Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA140.24875621890547264No Hit
CTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA140.24875621890547264RNA PCR Primer, Index 10 (96% over 28bp)
TCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA140.24875621890547264Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT130.23098791755508172TruSeq Adapter, Index 10 (96% over 26bp)
CTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA130.23098791755508172Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCT130.23098791755508172TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT120.21321961620469082RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT120.21321961620469082No Hit
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGC110.19545131485429992No Hit
TATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG110.19545131485429992Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT110.19545131485429992RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT100.17768301350390903No Hit
TCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.17768301350390903Illumina PCR Primer Index 10 (96% over 28bp)
CACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG100.17768301350390903Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCCACGAGAACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.17768301350390903Illumina PCR Primer Index 10 (96% over 28bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.17768301350390903No Hit
TCTCCGACCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA90.15991471215351813RNA PCR Primer, Index 29 (96% over 28bp)
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA90.15991471215351813Illumina PCR Primer Index 10 (96% over 28bp)
GCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA90.15991471215351813Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG90.15991471215351813TruSeq Adapter, Index 11 (96% over 29bp)
CATCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT90.15991471215351813RNA PCR Primer, Index 29 (96% over 27bp)
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA90.15991471215351813Illumina PCR Primer Index 11 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.14214641080312723No Hit
TCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.14214641080312723RNA PCR Primer, Index 29 (96% over 28bp)
TTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTC80.14214641080312723No Hit
TCTCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTG80.14214641080312723TruSeq Adapter, Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGT80.14214641080312723No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG80.14214641080312723TruSeq Adapter, Index 11 (96% over 30bp)
GAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAAAAA80.14214641080312723RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.14214641080312723RNA PCR Primer, Index 29 (96% over 28bp)
CATCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT80.14214641080312723TruSeq Adapter, Index 10 (96% over 26bp)
TCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTGAA70.12437810945273632RNA PCR Primer, Index 29 (96% over 27bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC70.12437810945273632RNA PCR Primer, Index 11 (96% over 27bp)
CACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG70.12437810945273632TruSeq Adapter, Index 10 (95% over 24bp)
TCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA70.12437810945273632TruSeq Adapter, Index 11 (96% over 30bp)
AGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA70.12437810945273632Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.12437810945273632RNA PCR Primer, Index 10 (96% over 28bp)
ATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT70.12437810945273632Illumina PCR Primer Index 10 (96% over 26bp)
TCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.12437810945273632Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTACGCTG70.12437810945273632No Hit
ACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT70.12437810945273632Illumina PCR Primer Index 10 (95% over 23bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG70.12437810945273632TruSeq Adapter, Index 11 (96% over 30bp)
TCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAA70.12437810945273632RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACAAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG70.12437810945273632Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTC70.12437810945273632No Hit
GATCGGAAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGC60.10660980810234541Illumina PCR Primer Index 11 (96% over 26bp)
TCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.10660980810234541RNA PCR Primer, Index 29 (96% over 28bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.10660980810234541No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG60.10660980810234541Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.10660980810234541Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTG60.10660980810234541TruSeq Adapter, Index 12 (96% over 26bp)
CATCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT60.10660980810234541TruSeq Adapter, Index 11 (96% over 29bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA60.10660980810234541RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.10660980810234541Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT60.10660980810234541Illumina PCR Primer Index 10 (95% over 21bp)
TCTCCGAGCCCACCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT60.10660980810234541Illumina PCR Primer Index 10 (96% over 27bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTGT156.2764157E-495.09
GGCTCGG156.2764157E-495.01
AAGAGAC205.161541E-447.49999618-19
TCTCCGA1505.9299055E-1031.6666681
CGAGACG300.003787635831.66666812-13
TTATACA803.9539862E-429.6874982
CTTATAC803.9539862E-429.6874981
CTCCGAG1702.2682798E-927.9411752
AGACGGA350.00804772827.14285514-15
ACGGAGC350.00804772827.14285516-17
TATACAC907.880351E-426.3888873
TCCGAGC2001.2867531E-823.753
CGAGCCC2501.9645086E-1022.8000015
TACACAT1050.00193696722.6190475
CCGAGCC2203.5453922E-821.590914
ATACACA1100.00253887821.590914
ACACATC1350.0083136417.5925926
GAGCCCA3753.085188E-815.26
AGCCCAC3955.8556907E-814.4303817
GTCTTCT4000.013.062538-39
Produced by FastQC (version 0.11.2)