FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s63.351000000d8313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s63.351000000d8313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT53.1055900621118013TruSeq Adapter, Index 9 (95% over 21bp)
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA31.8633540372670807Illumina PCR Primer Index 9 (100% over 27bp)
TCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAA31.8633540372670807TruSeq Adapter, Index 9 (96% over 28bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA31.8633540372670807RNA PCR Primer, Index 17 (96% over 28bp)
AGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAA21.2422360248447204RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACGATACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG21.2422360248447204Illumina PCR Primer Index 9 (96% over 28bp)
GATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC21.2422360248447204RNA PCR Primer, Index 47 (96% over 26bp)
TTATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTC21.2422360248447204No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT21.2422360248447204No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG21.2422360248447204RNA PCR Primer, Index 47 (96% over 29bp)
TCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG10.6211180124223602TruSeq Adapter, Index 9 (96% over 28bp)
CCCCAGGCGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC10.6211180124223602No Hit
CAGGTGATCACCATCGGCAACGAGCGTTTCCGCTGCCCCGAGGCCCTGTT10.6211180124223602No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT10.6211180124223602RNA PCR Primer, Index 41 (95% over 22bp)
CTACAATGGCCGGTACAATGGGTTGCGATACTGTGAGGTGGAGCTAATCC10.6211180124223602No Hit
TCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA10.6211180124223602TruSeq Adapter, Index 3 (96% over 28bp)
CGGAAGAGCACACGTCTCCCACGCGAGATCGGAAGAGCCCACGAGACCCT10.6211180124223602No Hit
TAATTTACGCGCCTGCTGCCTTCCTTAGATTTGGTAGCCGTTTCTCAGTC10.6211180124223602No Hit
CCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTT10.6211180124223602No Hit
TCTCCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT10.6211180124223602Illumina PCR Primer Index 9 (100% over 26bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACCCTAAGA10.6211180124223602No Hit
GGACCTTAGCGGGCGGTCCGGGCTGTTTCCCTCTCGAGCGCACAGCTTAG10.6211180124223602No Hit
TCTCCGAGCCCACGAGACATGCGCAGAAATCGTATGCCGTCTTTTGCTTG10.6211180124223602Illumina Single End Adapter 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACAGACATGCGCAGATCTCGTATGCCGTC10.6211180124223602No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT10.6211180124223602No Hit
CATCTCCGAGCCCACGAACATGCGCAGATCTCGTATGCCGTCTTCTGCTT10.6211180124223602RNA PCR Primer, Index 47 (96% over 28bp)
AGCACCCCGCTTGAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGT10.6211180124223602No Hit
TCTCCTAGCCCACGAGACATGCGCAGACCTCGTATGCCGTTTTCTGCTTG10.6211180124223602Illumina Paired End PCR Primer 2 (95% over 22bp)
CCCCTGCTCAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGC10.6211180124223602No Hit
CCTCTCCCAGTGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGT10.6211180124223602No Hit
GCCTCAAGAGCAGCAGCTGGGGCGCGGCCATCCAGGTCGGTGCCGACCTC10.6211180124223602No Hit
GGTGCGCTGTTTGGCCCGAGCCCACGAGACCCTAAGACACCTCGTATGCC10.6211180124223602No Hit
CATCTCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT10.6211180124223602RNA PCR Primer, Index 47 (96% over 28bp)
ACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGGGCTTCGATCAA10.6211180124223602No Hit
AAGTGAAACCGCAAAAGCCTCATTATATCAGATATGGTTCCTTAGATCGT10.6211180124223602No Hit
TCTACGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.6211180124223602RNA PCR Primer, Index 41 (96% over 29bp)
GCACCCCCCGTCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.6211180124223602No Hit
TCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAAGCAAGGC10.6211180124223602No Hit
CGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAA10.6211180124223602RNA PCR Primer, Index 47 (96% over 29bp)
CTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGA10.6211180124223602No Hit
ATACACATCTGCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT10.6211180124223602No Hit
GAGGAGCTCATAGTGCACCGATCCGATTCTCTCCTTGCGATCGTGTATGA10.6211180124223602No Hit
CCATAGTAGTGGGAACCACTTGAACTGGAACTGGCACCAGGACTGGCTGC10.6211180124223602No Hit
GAGCCCACAGACCCTAAGACACCTCGTATGCCGTCTTCTGCTTGAAAAAA10.6211180124223602TruSeq Adapter, Index 11 (96% over 26bp)
CGGATCGCCCAGATCTTCCCCGATGAGGCCCGGAAGGTCGGGGTCAAGGT10.6211180124223602No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG10.6211180124223602No Hit
CGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAA10.6211180124223602RNA PCR Primer, Index 41 (96% over 29bp)
TATACACATCTCCGAGCGCACGAGACATGCGCAGATCTCGTATGCCGTCT10.6211180124223602RNA PCR Primer, Index 47 (95% over 21bp)
GACGAACTCGATCACCATCGTGATGGACGGGGATGCGGTGCCGGTGAACG10.6211180124223602No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.6211180124223602RNA PCR Primer, Index 41 (96% over 29bp)
GGAGATGTGTATAAGAGACACACATCTCCGAGCCCACCAGACATGCGCAG10.6211180124223602No Hit
GGGCTCGGAGATGTATATAAGAGACAGCTCCGAGCCCACGAGACATGCGC10.6211180124223602No Hit
CTATTGGAGCTGGAATTTTTGCGGCTGCTGGCACCAGACTTGCCCTCCAA10.6211180124223602No Hit
CTCGGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA10.6211180124223602RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCACGAGGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAA10.6211180124223602RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCACCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT10.6211180124223602Illumina PCR Primer Index 9 (95% over 23bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACATGCGCA10.6211180124223602No Hit
GGTACAGACCGTTAAGATGCCTCCGGCAGCGCTTGGTCAGCTCCGTGGCC10.6211180124223602No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA10.6211180124223602No Hit
AAAAAAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC10.6211180124223602No Hit
CTTATACACATCTCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC10.6211180124223602No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTCGGAAGAGCCCCCCACACCAGA10.6211180124223602No Hit
GAGCACCCCGGTGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCG10.6211180124223602No Hit
ATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTT10.6211180124223602Illumina PCR Primer Index 9 (95% over 21bp)
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA10.6211180124223602No Hit
CACCCCACGTCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT10.6211180124223602No Hit
CATTCCGAGCCCACGAGACATGCGCAGCTCTCGTATGCCGTCTTCTGCTT10.6211180124223602Illumina PCR Primer Index 9 (96% over 26bp)
CCCCCGCCAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC10.6211180124223602No Hit
CATCTCCGAGGCCCACGAGACATGCGCAGGACCCGTATGCCGCCTTCTGC10.6211180124223602No Hit
CCCTGCGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC10.6211180124223602No Hit
CCCCCAAACAGATCGGAAGAGACCACGAGACCCTAAGACATCTCGTATGC10.6211180124223602No Hit
CTTATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10.6211180124223602No Hit
CGTTACCACCCCACCCCTATACATTCAGGGCAGGGCCACCGGACACTACT10.6211180124223602No Hit
GATACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAAT10.6211180124223602No Hit
GCACCCCGGGAGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.6211180124223602No Hit
TCCTGGGACTTGTCCCATGCCGCATTTACCACGGGCAATTTGATGATCCT10.6211180124223602No Hit
GTGGAGGCGTTGTTCTTGATGACCTTGCCGTCCTTGGTGTGGATGCCAGC10.6211180124223602No Hit
TCTCCTAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.6211180124223602RNA PCR Primer, Index 41 (96% over 29bp)
GTTCGGCGCCGTCATCACGGTGCCCGCCTATTTCGACGACGCGCAGCGCC10.6211180124223602No Hit
TGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATAT10.6211180124223602No Hit
CCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG10.6211180124223602No Hit
TCTCCGAGCCCACGAGACATGCGCAGAACTCGTATGCCGTCTTCTGCCTG10.6211180124223602Illumina PCR Primer Index 9 (95% over 24bp)
CACCCCGGGCCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT10.6211180124223602No Hit
ATTTTAGCTCCTCTGCTGGTCTGGCCTCTGACTAAAAATAAACAAGCCCT10.6211180124223602No Hit
CCAGCAGCGTCGTCACCAAAAAGGCCAGCGTGTAACGCGGCTGCCCCACG10.6211180124223602No Hit
GATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAGACATACCTCCC10.6211180124223602No Hit
GTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCA10.6211180124223602No Hit
CTTTAGAGGCGGCTCATTTGTAATGGTAGAAAAGTCGAACCGCTGCTCCA10.6211180124223602No Hit
CATCTCCGAGCCCACCGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC10.6211180124223602Illumina Paired End PCR Primer 2 (95% over 24bp)
GGTACAGAGAGTGGCGAGCCTGTGAGGGTGAGCGAATCTCGGAAAGCCGG10.6211180124223602No Hit
TTTTAACCGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCG10.6211180124223602No Hit
CAGCTTATCGCAGCTACTACGTCCTTCATCGCCTGATCGCCAAGGCATCC10.6211180124223602No Hit
ATGTATATCTGTATCGCGAATCCTTTGCCGCCGCTATCGGCATAGCGAGA10.6211180124223602No Hit
CTTATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGT10.6211180124223602No Hit
ACAGGGTGGGTTTCCCCATTCGGACATCCTAGTGTCAATGCGTATCCAGC10.6211180124223602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6211180124223602No Hit
GGGAAGCTTACGGATTTTGGAAATACAAAGAAAAAGCTAAAATCGAAGTA10.6211180124223602No Hit
ACATCTCCTAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC10.6211180124223602Illumina PCR Primer Index 9 (100% over 24bp)
CACATCTCCGAGCCCACTATACCCTAAGACATCTCGTATGCCGTCTTCTG10.6211180124223602RNA PCR Primer, Index 41 (96% over 25bp)
CTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA10.6211180124223602Illumina PCR Primer Index 3 (96% over 28bp)
TATCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG10.6211180124223602RNA PCR Primer, Index 47 (96% over 29bp)
ACCGAAGGCTGCAGAACAAACTGAAAGTCCTCCACAAGCTGAGCCCACTG10.6211180124223602No Hit
TCTACGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.6211180124223602TruSeq Adapter, Index 3 (96% over 28bp)
CGTCCCCGGTCGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.6211180124223602No Hit
CTTATACACATCTCCTAGCCCAGAGACCCTAAGACATCTCGTATGCCGTC10.6211180124223602No Hit
AAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTAATTG10.6211180124223602No Hit
GCTCGGAGATGTGTATAAGAGACAGCTGCGAGCCCACGAGACATGCGCAG10.6211180124223602No Hit
GCTTGATGATCGCGTGCGTCTCGGGCAGGTTCTCGTTGTTGGTGCCCTCG10.6211180124223602No Hit
ATCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGTTTCACTTTTA10.6211180124223602No Hit
CATCCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT10.6211180124223602TruSeq Adapter, Index 9 (96% over 27bp)
ATACACATCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT10.6211180124223602RNA PCR Primer, Index 47 (95% over 24bp)
GTATTGGAATACTATTTAGGGCCGAAAGGATTAGTAAACAAAGATTTTGA10.6211180124223602No Hit
GTAACGAGAAGGTATTAATCAATAAATTGACGGAATTATTAAAAGTCCTA10.6211180124223602No Hit
TCTCCAGCCCACGAGACCCTAAGACAGCTCGTATGCCGTCTTCTGCTTGA10.6211180124223602Illumina PCR Primer Index 11 (96% over 26bp)
AAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGAGGTAG10.6211180124223602No Hit
CATTTGTCCATTTTTATTAATTGTTCAACTTGCCCACAATAAAAAGCCAG10.6211180124223602No Hit
CTTATATGTGATTAAATACTTGTATTTTTTCATATGTTCCTACTATTTAA10.6211180124223602No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC10.6211180124223602RNA PCR Primer, Index 41 (96% over 26bp)
ATTATGGATTCCAAGTCATCGCCCCGCTGAGCTGTGTCGAAAGTGTTTTC10.6211180124223602No Hit
ATACACATCTCCGAGCCCACAGACCCTCAGACATCTCGTATGCCGTCTTC10.6211180124223602Illumina PCR Primer Index 3 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACACTGAGC10.6211180124223602No Hit
GACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAA10.6211180124223602No Hit
GGAGGAGCAGACCAGGGCATTGGTATTGGCCGGGTTGCCCACAACCAGCA10.6211180124223602No Hit
TCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG10.6211180124223602Illumina PCR Primer Index 9 (96% over 28bp)
GACTACGCTAAACAGTAACCCACATACGATCAACCGCATTCCGGTTCCAC10.6211180124223602No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATAT10.6211180124223602No Hit
TCTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG10.6211180124223602RNA PCR Primer, Index 47 (96% over 29bp)
CCTTCAGGTCCTCGCCCTCGCAGCTCCTGGGCTCGTGGCAAGCGCCGTAG10.6211180124223602No Hit
ATTTAATACAATGACAAAGCCTAGAATCAATTGTAAACGAAAAAAAAAAA10.6211180124223602No Hit
CCCCATGGCAGATCGGAAGAGCCCACGAGACCCTAAGACAGCTCGTATGC10.6211180124223602No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACCTGTCTC10.6211180124223602No Hit
TTATACACATCTCCGAGCCCACGAGCATGCGCAGATCTCGTATGCCGTCT10.6211180124223602RNA PCR Primer, Index 47 (95% over 21bp)
GCCTTGGTCGCTTTCGTCACTCTCTTCGCTGGCCGTTCCGTCGAGCGTCA10.6211180124223602No Hit
CTCTCCCTTTGGAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTA10.6211180124223602No Hit
CTCCGAGCCGACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA10.6211180124223602Illumina PCR Primer Index 9 (96% over 28bp)
CACCCCCTGGGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.6211180124223602No Hit
TCTCCGAGCCCACGAGACATGCGCAGACATCGTATGCCGTCTTCTGCTTG10.6211180124223602Illumina PCR Primer Index 1 (95% over 22bp)
GTGCCTCCTGAGGGCCCCATTGTGGGGTCTCCCGACACCGATCCGGGGAT10.6211180124223602No Hit
GTATTGTTATTTTTCGTCACTACCTCCCAGCTCGGGGACTGTCTCTTATA10.6211180124223602No Hit
TAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGGG10.6211180124223602No Hit
CCACCCATCATCACCACTGCCACTGCAATCACCATAGGCATCATCACCAC10.6211180124223602No Hit
GAGTAGACGGGCACCTGGTAGATGGCATTGAATCCAGTGACGGATCCAAT10.6211180124223602No Hit
GATATGAACTCTCCAAAAAAATTACGCTGTTATCCCTGTCTCTTATACAC10.6211180124223602No Hit
CATCTCCGAGCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT10.6211180124223602RNA PCR Primer, Index 47 (96% over 28bp)
CCCCTTGGAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC10.6211180124223602No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers