FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s61.351000000d82eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s61.351000000d82eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3358790
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT1223363.6422640296058995No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT907502.7018658504997335Illumina Paired End PCR Primer 2 (95% over 22bp)
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG689012.051363735154624TruSeq Adapter, Index 5 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT487251.4506712238633555Illumina Paired End PCR Primer 2 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC217440.6473759895676717No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT212390.6323408132095188Illumina Paired End PCR Primer 2 (96% over 27bp)
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGA167310.49812581316486Illumina Paired End PCR Primer 2 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT124830.3716516959976658Illumina Paired End PCR Primer 2 (95% over 21bp)
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTG124350.37022260992798TruSeq Adapter, Index 5 (95% over 24bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT85540.254675046668592No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG68650.20438908059152253No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC59910.1783678050726601Illumina Paired End PCR Primer 2 (96% over 26bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA59860.17821894194040117No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT56570.168423747837763No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54050.1609210459719125No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC51880.1544603860318746No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT49950.14871426912667954No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC49480.14731495568344552No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT48390.14406973940020068No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA47330.14091384099631118No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44670.13299432236013564No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG42970.12793297586333174No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42770.127337523334296No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG40780.12141277067039023No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA39400.11730414822004352No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA36520.10872963180192867No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA35480.10563327865094276No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC35420.10545464289223201No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA35400.10539509763932844No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA34590.10298351489673366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTT29800.030.7646875
CTTGTCT29450.029.5151449
ATCTTTT64150.029.469017
ATGCTTG30900.029.0545926
ACTTTAG7800.027.4315151
GTATAAG7900.027.0560598
TATATGC34400.026.788893
ATATGCT35000.026.4653744
CTTCCGA240850.025.6642531
CCGATCT238850.025.5955684
TTCCGAT241350.025.5666182
TCCGATC241450.025.4183123
GCTTGTC34200.025.415828
TGCTTGT34900.025.0421437
GTGTATA8200.024.909566
ATATAAG19200.024.2457872
TCTTTTT80600.023.6902758
CGATCTT107450.023.2536325
CTTTTTT81350.023.1799269
TTATATG39150.023.0533082
Produced by FastQC (version 0.11.2)