FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s60.351000000d82c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s60.351000000d82c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA195.21978021978022RNA PCR Primer, Index 41 (96% over 31bp)
TCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG71.9230769230769231RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG51.3736263736263736RNA PCR Primer, Index 41 (96% over 31bp)
TCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA51.3736263736263736RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG41.098901098901099Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTT41.098901098901099RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG30.8241758241758242Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA30.8241758241758242Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCAAGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG30.8241758241758242RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG30.8241758241758242RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT30.8241758241758242RNA PCR Primer, Index 41 (96% over 29bp)
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA30.8241758241758242Illumina PCR Primer Index 7 (96% over 27bp)
CACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG20.5494505494505495Illumina PCR Primer Index 3 (95% over 23bp)
CTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA20.5494505494505495RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT20.5494505494505495No Hit
TCTCCGAGCCCACCGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT20.5494505494505495RNA PCR Primer, Index 41 (96% over 30bp)
GTGTAAAGGCAGAAGGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGG20.5494505494505495No Hit
ATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT20.5494505494505495RNA PCR Primer, Index 41 (95% over 24bp)
TCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG20.5494505494505495RNA PCR Primer, Index 41 (96% over 31bp)
TTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCAACTTAG10.27472527472527475No Hit
CCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATGATTTGAC10.27472527472527475No Hit
CGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCG10.27472527472527475No Hit
CTGCTACCGCTTATGGATAGCCTCGCGGCGCATTAGCTAGTTGGTAGGGT10.27472527472527475No Hit
TGATATGCCGTCGGAAGCCGGGAGTACCATCTCCCAAGCCTAAATACTCC10.27472527472527475No Hit
GTGATAAACCGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTA10.27472527472527475No Hit
GTTTACTTAAGCCGCGCCGCCTCATATTCTGCGATCAATGATCGAGAATC10.27472527472527475No Hit
CTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
CTTCCGATCTGCTCGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTC10.27472527472527475No Hit
GTGGACGGGATTCTCACACGTCTTTCGCTACTCATACCGGCAGTCTCACT10.27472527472527475No Hit
CCGAACCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAA10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCAACTCGTATGCCGTCTT10.27472527472527475No Hit
ACCCTGGGGATAACAGGCTTATCTCCCTCAAGAGTTCACATCGACGGGGA10.27472527472527475No Hit
AGACTTAACAGAGTAAAACATTCTCTCTTGCTCATGCAATATTTTATAAA10.27472527472527475No Hit
CTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGT10.27472527472527475No Hit
TCTCCGAGCCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT10.27472527472527475RNA PCR Primer, Index 41 (96% over 30bp)
GCTCATAAGGGGCATGATGATTTGACGTCATCCCCGCCTTCCTCCGGTTT10.27472527472527475No Hit
CTTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGT10.27472527472527475RNA PCR Primer, Index 41 (95% over 21bp)
CATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAAGTACACACTA10.27472527472527475Illumina PCR Primer Index 6 (96% over 27bp)
ATACACATCTCCGACCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC10.27472527472527475Illumina PCR Primer Index 3 (95% over 21bp)
GATCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA10.27472527472527475Illumina PCR Primer Index 3 (96% over 27bp)
TAACATATATTTTGGGACCTTAGCTGGTGGTCTGGGCTGTTTCCCTTTCG10.27472527472527475No Hit
GGTTGATATTCCTGTACCACCTCAATTCGTTTTAAGCGATGGGGGGGACG10.27472527472527475No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGGAGCCATCTCGTATGCCGT10.27472527472527475No Hit
ACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT10.27472527472527475RNA PCR Primer, Index 41 (96% over 26bp)
GAGTGATTCCTTCATCCAGACCTTGAGCAGCCACTCCTTCGACGTCATCG10.27472527472527475No Hit
TACCTGTAGTCCCAGCTACTTGAGAGGCTGAGGTAGGAGGATCGCCTGAG10.27472527472527475No Hit
ACACATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCT10.27472527472527475RNA PCR Primer, Index 41 (96% over 26bp)
ATACACATCTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGTCTT10.27472527472527475RNA PCR Primer, Index 41 (95% over 24bp)
CGGTTAGGGTGGCGAGTTAGAGGGGTCATACCGTTAGGGTAGTATCTCAC10.27472527472527475No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT10.27472527472527475Illumina Paired End PCR Primer 2 (95% over 21bp)
GTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTA10.27472527472527475No Hit
GGCCGGTACTATTATTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAA10.27472527472527475No Hit
CCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACATCACCTT10.27472527472527475No Hit
TGACGGTACCCAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCG10.27472527472527475No Hit
ATACACATCTCCGAGCCCAAGACTCGACGTCATCTCGTATGCCGTCTTCT10.27472527472527475RNA PCR Primer, Index 41 (96% over 26bp)
TCTCCGAGCCCACGGGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
AGTTTTCCCAGTGCATGCTGGGAAAATAAAAGGTGGCTCTGCTACCGCTG10.27472527472527475No Hit
CTTATACACATCTCCGAGCCCACGACTCGACGTCATCTCGTATGCCGTCT10.27472527472527475No Hit
ATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCGT10.27472527472527475RNA PCR Primer, Index 41 (95% over 22bp)
GTATTCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGG10.27472527472527475No Hit
ATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAA10.27472527472527475No Hit
CTTATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT10.27472527472527475RNA PCR Primer, Index 41 (95% over 21bp)
CACCAGACTTGCCCTCCAATTGGTCCTTGTTCAAGGCTTTACAGTGTACT10.27472527472527475No Hit
CTTATACACATCTCCGAGCGCACGAGACTCGACGTCATTTCGTATGCCGT10.27472527472527475No Hit
CTCTTGGGGAATTGCCTCTGACAACAGAGTTGCCTTGCCCAAGGTCATGT10.27472527472527475No Hit
GGGCATGATGATTTGATGTCATCCCCACCTTCCTCCGGCTGTCTCTTATA10.27472527472527475No Hit
CGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCA10.27472527472527475No Hit
GATCAGAATGCCGCGGTGAATTCGTTCCCGGGTCTTGTACACACCGCCCG10.27472527472527475No Hit
GAGCCCACGAGACTCGACGTCAACTCGTATGCCGTCTTCTGCTTGAAAAA10.27472527472527475RNA PCR Primer, Index 45 (96% over 26bp)
CCGTATTATGTGAGCATGTATGTATATTCGCACGTATGTATGTATATGGA10.27472527472527475No Hit
AACCAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAA10.27472527472527475No Hit
TATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAACACACCTTG10.27472527472527475Illumina Paired End PCR Primer 2 (96% over 25bp)
ACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTG10.27472527472527475RNA PCR Primer, Index 41 (96% over 27bp)
CTTCCGATCTTCACGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTC10.27472527472527475No Hit
ACCCCATGGAGCTTTACTGTAGCTTGATATTGAGTGTTTTTACCGTTTGT10.27472527472527475No Hit
CATCCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTTCTT10.27472527472527475RNA PCR Primer, Index 41 (96% over 26bp)
ATTTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGACATATCTCGTA10.27472527472527475No Hit
GTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGC10.27472527472527475No Hit
GCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTCAACGCAACC10.27472527472527475Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGGGCCCACGAGACTCGACGTCCTATCGTATGCCGTCTTCTGCT10.27472527472527475TruSeq Adapter, Index 16 (95% over 23bp)
GGTTAGAAATCCAACACAGCTAGGGTAGTATCCCACCAACGCCTCCACGT10.27472527472527475No Hit
TTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTC10.27472527472527475No Hit
GATCTACGATTACTAGCGATTCCAGCTTCATGTAGTAGAGTTGCAGCTGT10.27472527472527475No Hit
GTAATCATCTTCCCTCCCCATCACTAGTCACCGTCGGTGCGAAGCCTTTC10.27472527472527475No Hit
GTTGGGTCCGTACCATCAGTCAACATCCTTTCTTTCTAGTGATTTCTTTC10.27472527472527475No Hit
TCTCCGGGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCCGAGCCCACGAGACTCGCAGCGATCTCGTATGCCGT10.27472527472527475No Hit
TCCAAACACAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAACAAAAACCGA10.27472527472527475No Hit
CTTCCGATCTACGACGGGGGGGACAGAATGACAGGTGGTGCATGGTTGTC10.27472527472527475No Hit
TCTCCGAGCCCACTAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTG10.27472527472527475TruSeq Adapter, Index 7 (96% over 26bp)
GTAAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGC10.27472527472527475No Hit
ACACATCTCAGAGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG10.27472527472527475Illumina PCR Primer Index 3 (95% over 23bp)
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA10.27472527472527475No Hit
CACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
ACATGGGTGAGTCACCGGCTATGTTGCCTAGGGCCCACACGGCCTGTTCC10.27472527472527475No Hit
CCTAAAAGGTAACGGAGGCGCTCAAAGGTTCCCTCAGAATGGTTGGAAAT10.27472527472527475No Hit
TCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTGAA10.27472527472527475TruSeq Adapter, Index 7 (96% over 26bp)
ATACACATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTT10.27472527472527475RNA PCR Primer, Index 41 (95% over 24bp)
ATACACATCTCGGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT10.27472527472527475No Hit
CTTCCGATCTGTGGTGGGACGGGCGGTGTGTACAAGCTGTCTCTTATACA10.27472527472527475No Hit
GGGTAGTATCCCACCAACGCCTCCACGTAAGCTAGCGCTCACGTATCTAA10.27472527472527475No Hit
GTATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGACATATCACGT10.27472527472527475No Hit
GAGTACGGCGGCACACGTGATATGTCGTCGGAAGCCGGGAGGACCATCTC10.27472527472527475No Hit
CTTCCGATCTCGGCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTC10.27472527472527475No Hit
CAGTCTTGCTCTGTCGCCCAGTCTGGAGTGCAGTGGCTGTCTCTTATACA10.27472527472527475No Hit
CGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAA10.27472527472527475Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTGAA10.27472527472527475TruSeq Adapter, Index 7 (96% over 26bp)
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCAAA10.27472527472527475No Hit
GTAGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAG10.27472527472527475No Hit
CCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGAAGCGACTGAGAGAGCCAT10.27472527472527475No Hit
GGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC10.27472527472527475No Hit
GATAGGAAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC10.27472527472527475Illumina PCR Primer Index 7 (95% over 24bp)
CTCCGAGCCCACGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAA10.27472527472527475Illumina PCR Primer Index 7 (96% over 27bp)
AGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTT10.27472527472527475No Hit
ACACATCTCCGAGCCCAAGACTCGACGTCATCTCGTATGCCGTCTTCTGC10.27472527472527475RNA PCR Primer, Index 41 (96% over 28bp)
GTCATTGGAAATGGTAACTTCACCGTCGCGAAAAACGACCTCGTTGTTGG10.27472527472527475No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGG10.27472527472527475No Hit
CTTCCGATCTGACCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTT10.27472527472527475No Hit
TCTAAGCGTGAAGCCCCTTTAAGATAAGATCTCCCATTCCAAAAAGGAAG10.27472527472527475No Hit
CTTCCGATCTCTACAGGGGGGGACAGAATGACAGGTGGTGCACTGTCTCT10.27472527472527475No Hit
GTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCCTG10.27472527472527475No Hit
ACATCTCCGAGCCCACGAGGCTCGACGTCATCTCGTATGCCGTCTTCTGC10.27472527472527475RNA PCR Primer, Index 41 (96% over 28bp)
GGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGT10.27472527472527475No Hit
AGAGTACAGTGGCGTGATCTTGGCTCACAGCAACCTCTGCCTCCCCCGAG10.27472527472527475No Hit
ATACACATCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG10.27472527472527475Illumina PCR Primer Index 7 (95% over 23bp)
GGGAATGCCGTGCCATAGACCAGCTCATTCTCCAGGAAGACCACTGGATC10.27472527472527475No Hit
CTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA10.27472527472527475Illumina PCR Primer Index 7 (96% over 27bp)
GCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTAGGCGGTTTTTTAAG10.27472527472527475No Hit
CCTATCATTAGATAGGCCGCAGTGAAAAGGTCCAAGCGACTGTTTATCAA10.27472527472527475No Hit
TCGCTCAACGTATAAAAGCTACCCCAGGGAGATCGGAAGAGCCCACGAGA10.27472527472527475No Hit
CCTCTCGGTCTACACCCTCGTCGAGATTCGATTCGCACAGCAGCAATCAC10.27472527472527475No Hit
GCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGACCTAT10.27472527472527475Illumina PCR Primer Index 10 (96% over 28bp)
CCCTTGGGACCGTCTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTG10.27472527472527475No Hit
AGATGGACGAGCTGCCAATGTCGCTCATGTCCAGGTTGAGGTCGTCCATG10.27472527472527475No Hit
CTCCGAGCCCAGGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
GTAACCGACCGAGCGGTCGGCCCATGAGGATCCTGAGTACCTGTCTCTTA10.27472527472527475No Hit
GGCGGTGTGTACAAGACTCGGGAACGTATTCACCGTAGCATGCTGATCTA10.27472527472527475No Hit
CTCAAAAACTTTGATTTCTCGTAAGGTGCCGATCGAGTCAAAAAGATAAC10.27472527472527475No Hit
GATTGTGTAGATTCATCTTCATTTAAGATTTCTACGTGACGTAATGTTTT10.27472527472527475No Hit
GACTTAAAAAACCGACTACGCGCGCTTTACGCCCAATAATTCCGGATAAC10.27472527472527475No Hit
GTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTAT10.27472527472527475No Hit
GATCTGTCCGTAGGCCATCGAAATCAACCTTGAGTTCACTAATTTATCGT10.27472527472527475No Hit
ATCTGCATCAGTGGTTGATCATAACTGGGACGTGTTCCCACACCTAAACC10.27472527472527475No Hit
CCGATCTGGACGGGGATTCTGGTTGATCCTGCCAGTAGTTATATGCTTGT10.27472527472527475No Hit
AGTCTGAACAGGGCATCGAGTAGGCGGTCGTCGACTCGAAACCAGGTGAC10.27472527472527475No Hit
GTTTAAGGTGAACACCCTGGAAAGGGTGGCCAGAGCAGGTGATAGCCCTG10.27472527472527475No Hit
CTTATACACATCTCCGAGCCACGAGAATCGACGTCATCTCGTATGCCGTC10.27472527472527475No Hit
CCCCTATGCAGATCGGAAGAGCCCACGAGACTCGACGTCAGCTCGTATGC10.27472527472527475No Hit
TCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
ATACACATCTCCGAGCCGACGAGACTCGACGTCATCTCGTATGCCGTCTT10.27472527472527475RNA PCR Primer, Index 41 (95% over 24bp)
GTTGTCGTCAGCTCGTGTCGAGATGTTGGGTTAAGTCCCGTAACGAGCGC10.27472527472527475No Hit
AGCCTCAAGTGATGATTATTGGAGGTAGAGCACTGTTTGGACGAGGGGTC10.27472527472527475No Hit
ATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTATTGTTAGTTGCCAG10.27472527472527475No Hit
TCTCAGAGCCCACGAGACTCGACGACATCTCGTATGCCGTCTTCTGCTTG10.27472527472527475RNA PCR Primer, Index 41 (100% over 31bp)
TCTCCGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
GAGTAGCTCCAGCGCCGGCGTTCTGGACATAACCAATAATGCGTCCGTTC10.27472527472527475No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGGGCTTCGATTCGT10.27472527472527475No Hit
CATCTCTGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT10.27472527472527475Illumina PCR Primer Index 7 (96% over 25bp)
CTCCAACGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAA10.27472527472527475No Hit
ACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT10.27472527472527475RNA PCR Primer, Index 41 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACGACGCCATCTCGTATGCCGT10.27472527472527475RNA PCR Primer, Index 41 (95% over 23bp)
CTACTAGATGGTTCGATTGGTCTTTCGCCCCTATACTCAATTCTGACAAT10.27472527472527475No Hit
GGAATATCAACCTGTTGACCATCGCTTACGCCATTCGGCCTCAGCTTAGG10.27472527472527475No Hit
ACTGTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGT10.27472527472527475No Hit
TATGAAAAGTTTAAATAAAGAATTCGGCAAAAATAATATTCACCTGTTTA10.27472527472527475No Hit
ATACACATCTCAGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTT10.27472527472527475No Hit
CACCCACCCTGACATGCCCATGATCGTCGTCAGCGCGGCCAGCGTGCCGA10.27472527472527475No Hit
GTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACT10.27472527472527475No Hit
CTCGTGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGT10.27472527472527475No Hit
GACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATG10.27472527472527475No Hit
ACTTTATGGGATTTGCTTGACCTCGCGGTTTCGCTGCCCTTTGTATTGTC10.27472527472527475No Hit
AACTCCAGCACTGAAGGGCGGAAACCCTCCAACACTTAGCACTCATCGTT10.27472527472527475No Hit
ACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC10.27472527472527475Illumina PCR Primer Index 3 (95% over 24bp)
TCGCTGGACAGAGATTCACTGCGGTACTGGAGAGTGCTGCTGCAGCAACT10.27472527472527475No Hit
CCCCAGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCA10.27472527472527475No Hit
ATGTTAACATTTAGCACAGTAGTTTTGGACAGCAAGATGCGACCATCCAC10.27472527472527475No Hit
CCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTGTAAAACAA10.27472527472527475RNA PCR Primer, Index 45 (96% over 26bp)
CATCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT10.27472527472527475Illumina PCR Primer Index 7 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTC10.27472527472527475RNA PCR Primer, Index 41 (95% over 22bp)
TCTCCGAGCCCACGAGACTCCGACGTCATCTCGTATGCCGTCTTCTGCTT10.27472527472527475RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCGAGACTCGACGTCAGCTCGTATGCCGTCT10.27472527472527475No Hit
CCTTATGCCTTTACACTCTGTGAATGATTTCCAACCATTCTGAGGGAACC10.27472527472527475No Hit
GACATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATCCCACA10.27472527472527475Illumina PCR Primer Index 6 (96% over 27bp)
TTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCC10.27472527472527475No Hit
GGTGGGCAGTTTGACTGGGGCGGTCGCCTCCCAAAGTGGAACGGAGGCGC10.27472527472527475No Hit
GCATTGCATCGACATCCCCGTGGCGCCAACTGCAGCAGCAGGTGCAGCAG10.27472527472527475No Hit
GTGCACACGTAATCCCAGCTACTTGGGAGGCTGAGACAGGAGAATCACTT10.27472527472527475No Hit
CCATCGAGGAGAACATCATCGATGACAAGGACCGTGTCGTTGTGCCCATA10.27472527472527475No Hit
CTCGTAAGGTGCCGATCGAGTCAAAAAGATAACATCGACCGATCCCTAGT10.27472527472527475No Hit
CAATAATTCCGGATAACGCTTGCCACCTACGTATTACCGCGACTGCTGGC10.27472527472527475No Hit
GCGATGTGCTCTATGATTACCTGAGCATCGTGAAGAATGCCTCCGACGAG10.27472527472527475No Hit
GACCTAGGTGACAGGTTGAGTAGGTGCAGCAAACCACCATGGCACACGTA10.27472527472527475No Hit
GTGTGATCTCGGCTCACTGCAACCTCTGAATTCAAGCCAGTCTCCTGCCT10.27472527472527475No Hit
GATCTTCACTGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTCACTA10.27472527472527475No Hit
GTATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGGCATATCACGT10.27472527472527475No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGCCATCTCGTATGCCGT10.27472527472527475No Hit
CATCTCGGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT10.27472527472527475Illumina PCR Primer Index 7 (96% over 25bp)
GGATGGTACAACGAGTGGCGAGCCCACGAGACTCGACGCATCTCGTATGC10.27472527472527475No Hit
GGAGTTAACGCATTAAGCACTCCGCCTGGGGATTACGACCGCAAGGTTGA10.27472527472527475No Hit
ATACACATCTCAGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTT10.27472527472527475No Hit
ATTTAGTTACTAGTTCTGTATATTCGTTTTCTTTCACAGTTTCTCTAATC10.27472527472527475No Hit
CATCTCCGAGCCCACGGGACTCGACGTCATCTCGTATGCCGTCTTCTGCT10.27472527472527475RNA PCR Primer, Index 41 (96% over 29bp)
GGTGCGGATTTGCTTGTGCAGTCGGTTGAGGGCGTCGAACTCGCGGCGAA10.27472527472527475No Hit
TCTCCGAGCCCACGAGACTCGACGTCCGATCGTATGCCGTCTTCTGCTTG10.27472527472527475Illumina Single End Adapter 1 (95% over 23bp)
GGATATTCCTCTTCACTTATCTTTAAAATGAATAAATGAGAGAAATACAA10.27472527472527475No Hit
AAATTACTCCAATGAGCATTTCCTTGTTTCATATTTTGGAGCATTTCAGA10.27472527472527475No Hit
CTCTAAGGGGGGGGGACAAAGTACAGGTGGTGCATGGTTGTCGTCAGCTC10.27472527472527475No Hit
TCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA10.27472527472527475Illumina PCR Primer Index 3 (96% over 27bp)
GTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGTAGATGGTCCTCCC10.27472527472527475No Hit
CTCTTCCGATCTGCAGAGGGGGATGCAGAAGACTTACTTGCTGAATTTAA10.27472527472527475No Hit
GGCCCAGGCGACTGTTTACCAAAAACACAGGTCTCTGCTAATTCGAAAGA10.27472527472527475No Hit
GTAGGAGAGCGTTCGATGGGCGAAGAAGCTGGACCGTCAGGACCAGTGGA10.27472527472527475No Hit
TCTCCTAGCCCACGATACTCGACGTCAAATCGTATGCCGTCTTCTGCTTG10.27472527472527475Illumina Single End Adapter 1 (95% over 22bp)
ACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCT10.27472527472527475RNA PCR Primer, Index 41 (96% over 26bp)
GGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTAGGCGGT10.27472527472527475No Hit
TCTCCGAGCCCACGAGACTCGACGTCCATCTCGTATGCCGTCTTCTGCTT10.27472527472527475TruSeq Adapter, Index 16 (96% over 28bp)
GTCTTGTACACACCGCCCGTCACACCACGAGAGTCTGAAACACCCGAACC10.27472527472527475No Hit
CCTTCATTGCGCCACGGCGGCTTTCGTGCGAACCCACGAGACTCGACGTC10.27472527472527475No Hit
AGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA10.27472527472527475Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTC10.27472527472527475Illumina PCR Primer Index 3 (95% over 21bp)
CTTCCGATCTACCCAGGGGGAAGAATAAGGACTACGCGTAGTTTGATGAC10.27472527472527475No Hit
GGTTGATATTCCTGTACTTCTTTCTTTCGTTTGACTGACGGAAGGACGCA10.27472527472527475No Hit
ACTTAGAGTGCCCAACTGAATGATGGCAACTAAGCTTAAGGGTTGCGCTC10.27472527472527475No Hit
CTCCAACGCCACCCTGATAGCCCAAGGCAAGTGTAGCCACCTTGCCACGT10.27472527472527475No Hit
TCTGCGAGCCCACGGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
GCCCATGAGGATCATGAGTACGACGGCACACGTGATATGCCGTCGGAAGC10.27472527472527475No Hit
GGACGCACCGCTGGTGCACCAGTTGTCATGCCCATGGCAGAGCTGGGTAG10.27472527472527475No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT10.27472527472527475Illumina PCR Primer Index 7 (95% over 22bp)
GGAGACGTGTGCTCTTCCGAAAAAAAAACAAAAAAGGACCGGAAGCGGCC10.27472527472527475No Hit
CATCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCT10.27472527472527475RNA PCR Primer, Index 41 (96% over 29bp)
GTGAAGAAGGTTTTCGGATCGTACCTCTGTTGTAAGAGAAGAACGAGTGT10.27472527472527475No Hit
TTTTTGTAGAGATGAGGTTTCACCATGTTCCCCACGCCAGATCGGAAGAG10.27472527472527475No Hit
GGCCTATTCACTGCGGCTGACTTGCGTCAGCGCCCCTTCTCCCGAAGTTA10.27472527472527475No Hit
CTTATACACATCCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC10.27472527472527475RNA PCR Primer, Index 41 (95% over 22bp)
CTTCCGATCTGCTCAGGGTCCTCATGGGTCGACCGCTCGGTCGGTTACAG10.27472527472527475No Hit
TCTCCTAAGGCTAAATACTCTCTAGTGACCGATAGTGAACCAGCCCGCGA10.27472527472527475No Hit
CTGTTGGACTAGCGCGTCCAAGCTAGGAGCTAGGTGGGGAGTGAAAGGCT10.27472527472527475No Hit
CCGCCTCTTCCTGCAAGCCCGTATCAGATCGGAAGAGCACCTGTCTCTTA10.27472527472527475No Hit
TTATAGTACCTGTGAAGATGCAGGTTACCCGCGACAGGACGGAAAGACCC10.27472527472527475No Hit
CCTGTGAAGATGCAGGTTACCCGCGACAGGACGGAAAGACCCCATGGAGA10.27472527472527475No Hit
TATTGAGTGTTTGTACCGTTTGTACAGGATAGGTAGGAGCCAAGGAACTC10.27472527472527475No Hit
GTTCCGACCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA10.27472527472527475Illumina PCR Primer Index 3 (96% over 27bp)
TCTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTG10.27472527472527475TruSeq Adapter, Index 7 (96% over 26bp)
TCTCCGAGCCCAGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA10.27472527472527475RNA PCR Primer, Index 41 (96% over 31bp)
GTGTAGCCCAAATCATAAGGGGCATGATGATTTGACGTCATCCCCACCTT10.27472527472527475No Hit
CATCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT10.27472527472527475Illumina PCR Primer Index 7 (96% over 26bp)
GATCTGGCGGGGGACCGACCGTTTAAGGTGAACACCCTGGGAAGGGTGGC10.27472527472527475No Hit
GTGTGACTCCCGGACTGGCATGGGTGGCATTCGGAGTTTATCAGGATTCG10.27472527472527475No Hit
ATACACATCTCCGAGCCCAGAGACTCGACGTCATCTCGTATGCCGTCTTC10.27472527472527475RNA PCR Primer, Index 41 (96% over 25bp)
TCTCAGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTG10.27472527472527475TruSeq Adapter, Index 7 (96% over 26bp)
CTCCCAAGCCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGA10.27472527472527475No Hit
GTGCCCAACTGAATGATGGCAACTAAGCTTAAGGGTTGCGCTCGTTGCGG10.27472527472527475No Hit
GGCTACACACGTGCTACAATGGACAATACAAAGGGCAGCGAAACCGCGAG10.27472527472527475No Hit
ATACACATCTCCGAGCCCACGCTCGACGTCATCTCGTATGCCGTCTTCTG10.27472527472527475RNA PCR Primer, Index 41 (96% over 27bp)
AAGTCACAGCGAATTTTCCAGTTTAATTCGTCATCATCGAGCTGGACAAC10.27472527472527475No Hit
GCCCAAATCATAAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTC10.27472527472527475No Hit
GTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGAAAAACTT10.27472527472527475No Hit
AAACCGCGAGGTCAAGCAAATCCCATAAAGTCTGTCTCTTATACACATCT10.27472527472527475No Hit
CCCTGGGAAGGGTGGCCAGAGCAGGTGATAGCCCTGTAACCGACCGAGCG10.27472527472527475No Hit
CTTCCCACCCAGTTAGGGTGGCGAGTTAGAGGGGTCATACCGTTAGGGTA10.27472527472527475No Hit
TTTATAATCCCAGTGCTTTGGGAGGCTGAAGTGGGAGGATCACCCGAGCC10.27472527472527475No Hit
CCCTACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT10.27472527472527475No Hit
TCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA10.27472527472527475Illumina PCR Primer Index 7 (96% over 27bp)
GTATTGTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATG10.27472527472527475No Hit
GTGTAAAGGCATAAGGGAGCTTGACTGCGAGACTTACAAGTCGAGCAGGG10.27472527472527475No Hit
ACCATCTCCCAAGCCTAAATACTCCCTAGTGACCGATAGTGAACCAGTAC10.27472527472527475No Hit
GCTTGAATGGGCGCTTTTCTGGCAACGGGAGGCTAAAGGCGGTAATCTTT10.27472527472527475No Hit
AGGTCAAGCAAATCCCATAAAGTTGTTCTCAGTTCGGATTGTAGTCTGCA10.27472527472527475No Hit
CCCTGCAGGAGGCCATCGCCGCCGGCGCCGATGCCGTCTACAGCGGCCTG10.27472527472527475No Hit
CAGGTGATCTGCCCACCTTGGCCTCCCAAGGCGCTGGGATTACAGGTGTG10.27472527472527475No Hit
ACACATCTCCGAGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG10.27472527472527475RNA PCR Primer, Index 41 (96% over 27bp)
CTTGACGGGGAAGCCACGAATCGAAGCCCCAGTAAACGGCGGCCGTAACA10.27472527472527475No Hit
GTGGCGTAGAGAACGACGTTGTCGGGCCGGCCATCGATGCCGCCTTCCAG10.27472527472527475No Hit
CATTCAGCAGTACGACAGCAAGAATCCGGTTGCCGGAATCGTCGTTGAAC10.27472527472527475No Hit
GGATAGGTAGGAGCCAAGGAACTCTGGACGCTAGTCTAGAGGGAGGCATT10.27472527472527475No Hit
AACGTGCATTTGGTGACCTTCGGAAACACCCGCGCCATGGGATCAATGCG10.27472527472527475No Hit
ATACACATCTCCGAGCCCACGAGATTCGACGTCATCTCGTATGCCGTCTT10.27472527472527475RNA PCR Primer, Index 41 (95% over 24bp)
TCCTGGGGCTGAAGTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGC10.27472527472527475No Hit
TTATAGTACCTGTGAAGATGCAGGTTACCCGCGGTAGATCGGAAGAGCCC10.27472527472527475No Hit
TCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTG10.27472527472527475TruSeq Adapter, Index 7 (96% over 26bp)
GCTCATCGCATCCTGGGGCTGAAGTCGGTCCCAAGGGTTGGGCTGTTCGC10.27472527472527475No Hit
GCGGGGCGAGCACGAGGTGCTGGGACTCGCGCACATCACGGGCGGTGGCT10.27472527472527475No Hit
AAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTGTCT10.27472527472527475No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGGCTTCGATTCGT10.27472527472527475No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGCATCCCGTCCCCT10.27472527472527475No Hit
CATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT10.27472527472527475RNA PCR Primer, Index 41 (96% over 29bp)
TGCATGTCTGGGTTGTAGGACTCAATGTCGGGACCGGCCGTTTAAGGTGA10.27472527472527475No Hit
CGACATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACAAG10.27472527472527475RNA PCR Primer, Index 13 (96% over 27bp)
ATTCTAAGGTGAGCGAGCGAACTCTCGTTAAGGAACTCGGCAAAATGACC10.27472527472527475No Hit
GTGCCAAACCTCCCCGTCGATGTGAACTCTTGGGGGAGATAAGCCTGTTA10.27472527472527475No Hit
GGCTTGGGAGATGGTCCTCCCGGCTTCCGACGACATATCTCGTATGCCGT10.27472527472527475No Hit
ATACACATCTCCGAGCCCACGAGACTCTGCGCGATCTCGTATGCCGTCTT10.27472527472527475Illumina PCR Primer Index 1 (95% over 21bp)
CCCAATGCAGATCGGAAGAGCACACGTCTCCCAATGCCTGTCTCTTATAC10.27472527472527475Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGAAC10.27472527472527475No Hit
GATCACGAGATCGTTCCGACATCTGCCTAGCAATCTGCGCAAATCGCGGC10.27472527472527475No Hit
GAAGAGGAAAGTGGAATTCCATGTGTCGCGGTGAAATGCGCAGAGATATG10.27472527472527475No Hit
GTCTATGTGTTTGTTTTTGTACCAGTACGATGCCATGTTGGTTACTGTAG10.27472527472527475No Hit
GGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGCTGTCTCT10.27472527472527475No Hit
GTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACA10.27472527472527475No Hit
GTCCTCCCGGCTTCCGACGACATATCTCGTATGCCGTCTTCTGCTTGAAA10.27472527472527475TruSeq Adapter, Index 6 (96% over 27bp)
CCCTTAAGCTTAGTTGCCATCATTCAGTTGGGCACTCTAAGTTGACTGCC10.27472527472527475No Hit
CCATTGGGGGTAGAGCACTGTTTTCGGCTAGGGGGTCATCTCGACTTACC10.27472527472527475No Hit
TACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGAAGCGACTGAGAG10.27472527472527475No Hit
ACCTGCATCTTCACAGGTACTATAATTTCACCGAGTCTCTCGTTGAGACA10.27472527472527475No Hit
ACACATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCCTCA10.27472527472527475No Hit
GTGTAGCCCAGCTCATAAGGGGCATGATGATTTGACGTCATCCCCGCCTT10.27472527472527475No Hit
TACACATTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT10.27472527472527475RNA PCR Primer, Index 41 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1900.0040932718.7554-55