FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s6.351000000d7bcb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s6.351000000d7bcb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3638
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3489.565695437053327No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471.291918636613524No Hit
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA461.2644310060472788Illumina PCR Primer Index 5 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGC371.017042330951072Illumina PCR Primer Index 10 (96% over 26bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC350.9620670698185816RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC340.9345794392523363RNA PCR Primer, Index 12 (96% over 26bp)
GAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA310.8521165475536009Illumina PCR Primer Index 10 (96% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.7971412864211105No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.4123144584936778No Hit
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA130.35733919736118747TruSeq Adapter, Index 12 (96% over 29bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA120.32985156679494226RNA PCR Primer, Index 17 (96% over 28bp)
GAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAA110.30236393622869706Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG110.30236393622869706No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT100.2748763056624519No Hit
ATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTT70.19241341396371633Illumina PCR Primer Index 12 (95% over 22bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG70.19241341396371633TruSeq Adapter, Index 12 (96% over 29bp)
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA60.16492578339747113TruSeq Adapter, Index 3 (96% over 28bp)
AGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTG60.16492578339747113Illumina PCR Primer Index 10 (96% over 25bp)
TCTCCTAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG60.16492578339747113TruSeq Adapter, Index 12 (96% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAA60.16492578339747113No Hit
AAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT60.16492578339747113No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG60.16492578339747113No Hit
AGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG50.13743815283122596Illumina PCR Primer Index 5 (95% over 24bp)
CTTATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT50.13743815283122596No Hit
AAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT50.13743815283122596TruSeq Adapter, Index 12 (95% over 24bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG50.13743815283122596No Hit
GCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG50.13743815283122596Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT50.13743815283122596Illumina PCR Primer Index 10 (95% over 21bp)
AGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG40.10995052226498077RNA PCR Primer, Index 17 (95% over 24bp)
TCTCCGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG40.10995052226498077Illumina PCR Primer Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAACGCGTAGTAATCTCGTATGCCGTC40.10995052226498077No Hit
CTTATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGT40.10995052226498077No Hit
TCTCCGAGCCAACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG40.10995052226498077TruSeq Adapter, Index 10 (96% over 29bp)
ATACACATCTCCGAGCACACGAGACGCGTAGTAATCTCGTATGCCGTCTT40.10995052226498077Illumina PCR Primer Index 12 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10995052226498077No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT40.10995052226498077No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10995052226498077No Hit
AAAAAAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC40.10995052226498077No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA300.00951516347.51
CTTTTTT550.002254221134.5454561
AGAGCCC1002.313509E-416.62518-19
AGATCGG1104.7873225E-415.11363610-11
ATCGGAA1156.712359E-414.45652212-13
GAAGAGC1209.270106E-413.85416716-17
CGGAAGA1209.270106E-413.85416714-15
TGCTTGA1300.001697728212.78846258-59
CTTGAAA1300.001697728212.78846260-61
TGAAAAA1350.002255984312.31481562-63
ATCTCGT1400.00296507611.87499940-41
CACGAGA1400.00296507611.87499924-25
TCTGCTT1400.00296507611.87499956-57
GTCTTCT1400.00296507611.87499952-53
CTTCTGC1400.00296507611.87499954-55
TATGCCG1450.00385746211.46551746-47
CGTATGC1450.00385746211.46551744-45
CTCGTAT1450.00385746211.46551742-43
TGCCGTC1500.004970911.08333448-49
CCGTCTT1550.0063490210.72580550-51
Produced by FastQC (version 0.11.2)