FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s59.351000000d82a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s59.351000000d82a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA252.2163120567375887Illumina PCR Primer Index 11 (100% over 29bp)
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA171.5070921985815602Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA161.4184397163120568TruSeq Adapter, Index 11 (96% over 30bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA100.8865248226950355RNA PCR Primer, Index 11 (96% over 30bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA100.8865248226950355RNA PCR Primer, Index 40 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC100.8865248226950355RNA PCR Primer, Index 11 (96% over 27bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC100.8865248226950355RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG70.6205673758865249TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG70.6205673758865249Illumina PCR Primer Index 11 (96% over 30bp)
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG70.6205673758865249TruSeq Adapter, Index 11 (96% over 30bp)
ACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT60.5319148936170213Illumina PCR Primer Index 11 (100% over 24bp)
TCTCCGAGACCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.5319148936170213Illumina PCR Primer Index 11 (96% over 30bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA60.5319148936170213RNA PCR Primer, Index 17 (96% over 28bp)
TCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.5319148936170213TruSeq Adapter, Index 11 (96% over 30bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA60.5319148936170213RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.44326241134751776Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG50.44326241134751776RNA PCR Primer, Index 29 (96% over 30bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA50.44326241134751776TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT50.44326241134751776TruSeq Adapter, Index 11 (95% over 23bp)
TCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA50.44326241134751776TruSeq Adapter, Index 11 (96% over 30bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA50.44326241134751776Illumina PCR Primer Index 10 (96% over 29bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA50.44326241134751776RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT50.44326241134751776No Hit
TATCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.3546099290780142RNA PCR Primer, Index 11 (96% over 30bp)
CCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAA40.3546099290780142RNA PCR Primer, Index 29 (96% over 30bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCT40.3546099290780142No Hit
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA40.3546099290780142RNA PCR Primer, Index 29 (96% over 30bp)
CTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.3546099290780142RNA PCR Primer, Index 10 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT30.26595744680851063TruSeq Adapter, Index 10 (95% over 22bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA30.26595744680851063No Hit
TCTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG30.26595744680851063TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT30.26595744680851063No Hit
TCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.26595744680851063Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT30.26595744680851063Illumina PCR Primer Index 11 (96% over 29bp)
GAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA30.26595744680851063TruSeq Adapter, Index 11 (96% over 30bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.26595744680851063No Hit
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.26595744680851063TruSeq Adapter, Index 11 (96% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.26595744680851063No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACCCTAAGA30.26595744680851063No Hit
CATCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT30.26595744680851063TruSeq Adapter, Index 11 (96% over 29bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC30.26595744680851063RNA PCR Primer, Index 6 (96% over 25bp)
TCTCCGAGCCCACTAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG30.26595744680851063RNA PCR Primer, Index 41 (96% over 29bp)
CGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA30.26595744680851063TruSeq Adapter, Index 11 (96% over 30bp)
CATCTCCGAGCCCACGGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT30.26595744680851063Illumina PCR Primer Index 11 (100% over 28bp)
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT20.1773049645390071Illumina PCR Primer Index 10 (95% over 22bp)
TATCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.1773049645390071RNA PCR Primer, Index 29 (96% over 30bp)
CTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA20.1773049645390071Illumina PCR Primer Index 11 (100% over 29bp)
ACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT20.1773049645390071Illumina PCR Primer Index 11 (100% over 24bp)
CTTATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTC20.1773049645390071Illumina PCR Primer Index 11 (95% over 21bp)
CACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG20.1773049645390071TruSeq Adapter, Index 11 (96% over 26bp)
GCACCCCGCCTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA20.1773049645390071No Hit
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.1773049645390071TruSeq Adapter, Index 10 (96% over 29bp)
TCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA20.1773049645390071Illumina PCR Primer Index 11 (96% over 30bp)
CACATCTCCGACCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC20.1773049645390071Illumina PCR Primer Index 11 (96% over 27bp)
CATCTCCGAGCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT20.1773049645390071Illumina PCR Primer Index 11 (96% over 29bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC20.1773049645390071RNA PCR Primer, Index 41 (96% over 26bp)
CATCTCCGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT20.1773049645390071Illumina PCR Primer Index 3 (96% over 26bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1773049645390071No Hit
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.1773049645390071TruSeq Adapter, Index 10 (96% over 29bp)
TCTCCGAACCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.1773049645390071Illumina PCR Primer Index 11 (96% over 30bp)
ACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTCTTCTG20.1773049645390071Illumina PCR Primer Index 11 (96% over 26bp)
GCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA20.1773049645390071Illumina PCR Primer Index 10 (96% over 29bp)
CTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1773049645390071Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1773049645390071No Hit
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.1773049645390071TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.1773049645390071RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTT20.1773049645390071Illumina PCR Primer Index 6 (96% over 27bp)
GCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA20.1773049645390071RNA PCR Primer, Index 17 (96% over 28bp)
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.1773049645390071Illumina PCR Primer Index 10 (96% over 29bp)
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.1773049645390071Illumina PCR Primer Index 10 (96% over 29bp)
CTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1773049645390071Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT20.1773049645390071No Hit
ACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT20.1773049645390071Illumina PCR Primer Index 10 (95% over 24bp)
ATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT20.1773049645390071TruSeq Adapter, Index 11 (95% over 23bp)
CTCCGAGCCCACGAGACGGAGCTACAGCTCGTATGCCGTCTTCTGCTTGA20.1773049645390071Illumina PCR Primer Index 11 (96% over 29bp)
ACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCT20.1773049645390071Illumina PCR Primer Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGACGGAGCTACATCTCGTATGCCGTCT20.1773049645390071TruSeq Adapter, Index 11 (95% over 22bp)
TCTCCGAGCCCACGATACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG20.1773049645390071RNA PCR Primer, Index 40 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG200.001564290371.252
CATCTCC200.001564290371.251
CGAGCCC250.00379283257.0000045
CCGAGCC250.00379283257.0000044
AAAAAAA5302.9130751E-77.16981156-57
Produced by FastQC (version 0.11.2)