FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s57.351000000d8264.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s57.351000000d8264.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA795.183727034120735Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA563.674540682414698Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGCT312.0341207349081363No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG181.1811023622047243Illumina PCR Primer Index 10 (96% over 28bp)
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA171.1154855643044619Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG161.0498687664041995TruSeq Adapter, Index 10 (96% over 28bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA150.984251968503937Illumina PCR Primer Index 10 (96% over 28bp)
TCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG140.9186351706036745Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT140.9186351706036745No Hit
TCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG140.9186351706036745TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT130.8530183727034121Illumina PCR Primer Index 10 (96% over 27bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA130.8530183727034121Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG130.8530183727034121Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC120.7874015748031495Illumina PCR Primer Index 10 (96% over 25bp)
TCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG120.7874015748031495Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTGAGCCCACGAGACACTCGCTAATC110.7217847769028871No Hit
TCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG110.7217847769028871TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTT110.7217847769028871TruSeq Adapter, Index 10 (95% over 21bp)
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA90.5905511811023622Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGT80.5249343832020997No Hit
ACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT80.5249343832020997Illumina PCR Primer Index 10 (95% over 23bp)
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.45931758530183725RNA PCR Primer, Index 29 (96% over 28bp)
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.45931758530183725Illumina PCR Primer Index 10 (96% over 28bp)
GAAGAGCACACGTCTCCCAGTGCAGATCGGAAGAGCACACGTCTCCCTGT70.45931758530183725Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT70.45931758530183725Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG70.45931758530183725Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCT60.39370078740157477RNA PCR Primer, Index 10 (95% over 23bp)
CATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT60.39370078740157477Illumina PCR Primer Index 10 (96% over 26bp)
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT60.39370078740157477TruSeq Adapter, Index 10 (96% over 26bp)
CTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.39370078740157477Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT60.39370078740157477Illumina PCR Primer Index 10 (95% over 23bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA60.39370078740157477Illumina PCR Primer Index 11 (100% over 29bp)
TATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG60.39370078740157477Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT50.32808398950131235No Hit
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA50.32808398950131235Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTGAGCCCACGAGACACTCGCTAATCTCGTA50.32808398950131235No Hit
CATTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.32808398950131235Illumina PCR Primer Index 10 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCCCACGAGACACTCGCTAA50.32808398950131235No Hit
CATCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.32808398950131235Illumina PCR Primer Index 10 (96% over 27bp)
ATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT50.32808398950131235Illumina PCR Primer Index 10 (95% over 21bp)
CTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.32808398950131235RNA PCR Primer, Index 10 (96% over 28bp)
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.32808398950131235Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA40.26246719160104987No Hit
CATCTCCGAGCCCACGAGACACTGAGCCCACGAGACACTCGCTAATCTCG40.26246719160104987No Hit
TATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCT40.26246719160104987No Hit
ATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTT40.26246719160104987TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.26246719160104987RNA PCR Primer, Index 29 (96% over 28bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGC40.26246719160104987No Hit
ACACATCTCCGAGCCGACGAGACACTCGCTAATCTCGTATGCCGTCTTCT40.26246719160104987RNA PCR Primer, Index 10 (95% over 23bp)
GCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA40.26246719160104987Illumina PCR Primer Index 10 (96% over 28bp)
CTTTTTATCGGTGATATTCCGTCCCTCGGCCAACAAATATATTATGTTTC40.26246719160104987No Hit
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA40.26246719160104987Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTT30.19685039370078738RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.19685039370078738RNA PCR Primer, Index 29 (96% over 28bp)
ACTCTGTGAGGCTGACTGTTACTATTTCTACTTTACAGATGAGGAAACTG30.19685039370078738No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCG30.19685039370078738No Hit
TCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.19685039370078738Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCT30.19685039370078738TruSeq Adapter, Index 10 (96% over 26bp)
CTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.19685039370078738Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCATGAGACACTCGCTAATCTCGTATGCCGTCTT30.19685039370078738TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT30.19685039370078738Illumina PCR Primer Index 10 (95% over 21bp)
CTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.19685039370078738RNA PCR Primer, Index 10 (96% over 28bp)
CATCTCCGAGCCCCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT30.19685039370078738RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACGAGACACTCGCTAAGCTCGTATGCCGTCTTCTGCTTG30.19685039370078738TruSeq Adapter, Index 12 (96% over 26bp)
TCTCCGAGCCCACGATACACTCGCTAACCTCGTATGCCGTCTTCTGCTTG30.19685039370078738RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG30.19685039370078738TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT30.19685039370078738Illumina PCR Primer Index 10 (96% over 27bp)
GGCTCGGAGATGTGTATAAGAGACAGCATCTCCGAGCCCACGAGACACTC30.19685039370078738No Hit
TCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAA30.19685039370078738RNA PCR Primer, Index 29 (96% over 27bp)
CTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA30.19685039370078738TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT30.19685039370078738Illumina PCR Primer Index 10 (96% over 26bp)
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA30.19685039370078738Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA30.19685039370078738TruSeq Adapter, Index 10 (96% over 28bp)
ATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCAAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493Illumina PCR Primer Index 10 (96% over 28bp)
CTCCAAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.13123359580052493Illumina PCR Primer Index 10 (96% over 28bp)
TACACATTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT20.13123359580052493Illumina PCR Primer Index 10 (95% over 23bp)
TCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCTAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCCCACCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.13123359580052493TruSeq Adapter, Index 10 (96% over 28bp)
TCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTGAA20.13123359580052493RNA PCR Primer, Index 29 (96% over 27bp)
CACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG20.13123359580052493Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493Illumina PCR Primer Index 11 (96% over 29bp)
TCTCTGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTC20.13123359580052493No Hit
GAGCCCACGAGACACTCGCTAAGCTCGTATGCCGTCTTCTGCTTGAAAAA20.13123359580052493RNA PCR Primer, Index 29 (96% over 27bp)
ACACATCTCCGAGCCCAAGAGACACTCGCTAATCTCGTATGCCGTCTTCT20.13123359580052493RNA PCR Primer, Index 10 (95% over 23bp)
ATACACATCTGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT20.13123359580052493Illumina PCR Primer Index 10 (95% over 23bp)
TTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC20.13123359580052493No Hit
TCTCCGAGCCCACGTGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493TruSeq Adapter, Index 10 (96% over 28bp)
TATACACATCTCTGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT20.13123359580052493No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13123359580052493No Hit
CATCTCCGGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT20.13123359580052493Illumina PCR Primer Index 10 (96% over 27bp)
CATCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT20.13123359580052493Illumina PCR Primer Index 10 (96% over 27bp)
CTTATACACATCTCCGAGCCCAAGAGACACTCGCTAATCTCGTATGCCGT20.13123359580052493No Hit
CATCTCCGAAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC20.13123359580052493Illumina PCR Primer Index 10 (96% over 25bp)
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT20.13123359580052493RNA PCR Primer, Index 29 (96% over 27bp)
CTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.13123359580052493Illumina PCR Primer Index 10 (96% over 28bp)
GTTTGGGTGGATATGTACTTTACCTGGGTACGTGAGAAACGGATGAATCC20.13123359580052493No Hit
ATACACATATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT20.13123359580052493Illumina PCR Primer Index 10 (95% over 21bp)
TTTTTAAAATGTCTGATTCAGGTGGTACACGTGTAGGTTTTTATATGGGT20.13123359580052493No Hit
ACACATCTACGAGCCCACGAGACACTCGCTAATATCGTATGCCGTCTTCT20.13123359580052493RNA PCR Primer, Index 29 (95% over 22bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13123359580052493No Hit
ACACATCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCT20.13123359580052493RNA PCR Primer, Index 11 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT20.13123359580052493Illumina Paired End PCR Primer 2 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13123359580052493No Hit
TTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.13123359580052493Illumina PCR Primer Index 10 (96% over 28bp)
CTCAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA20.13123359580052493Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.13123359580052493TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493TruSeq Adapter, Index 11 (96% over 30bp)
GGATGCTGGTCACGTCGTCGCTGTCCATGATCGAGAACGCCGGCTTGAGG20.13123359580052493No Hit
CATCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTT20.13123359580052493RNA PCR Primer, Index 29 (96% over 27bp)
GAGCACACGTCTCCCCGCCTAGATCGGAAGAGCACACGTCTCCCCGCCTA20.13123359580052493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA20.13123359580052493TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACACTGAGCCACGAGACACTCGCTAATCTCGTAT20.13123359580052493No Hit
ATACACATCTCCGAGCCCAAGACACTCGCTAATCTCGTATGCCGTCTTCT20.13123359580052493RNA PCR Primer, Index 10 (95% over 23bp)
ATCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.13123359580052493TruSeq Adapter, Index 10 (96% over 28bp)
TTATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTC20.13123359580052493No Hit
TCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.13123359580052493Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.13123359580052493Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT20.13123359580052493Illumina PCR Primer Index 10 (96% over 26bp)
CATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT20.13123359580052493TruSeq Adapter, Index 10 (96% over 26bp)
CTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.13123359580052493TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT20.13123359580052493RNA PCR Primer, Index 29 (96% over 27bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAGG20.13123359580052493No Hit
CGGAGATGTGTATAAGAGACAGGGCTCGGAGATGTGTATAAGAGACAGAC20.13123359580052493No Hit
AGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.13123359580052493Illumina PCR Primer Index 11 (96% over 29bp)
ACACATCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTG20.13123359580052493RNA PCR Primer, Index 10 (95% over 24bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA20.13123359580052493Illumina PCR Primer Index 11 (96% over 29bp)
GGCATCGACAGATCCGCCGATGGCGCCCATGTCACCATCGAGCAACTGTC20.13123359580052493No Hit
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT20.13123359580052493No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC200.001692124571.253
TTATACA200.001692124571.252
CTTATAC200.001692124571.251
Produced by FastQC (version 0.11.2)