FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s55.351000000d8221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s55.351000000d8221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA74.487179487179487Illumina PCR Primer Index 9 (100% over 27bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACATGCGCA31.9230769230769231No Hit
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA31.9230769230769231RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG31.9230769230769231RNA PCR Primer, Index 47 (96% over 29bp)
CTTATACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT21.282051282051282No Hit
TCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAA21.282051282051282TruSeq Adapter, Index 9 (96% over 28bp)
TCTCCTAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG21.282051282051282RNA PCR Primer, Index 47 (96% over 29bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC21.282051282051282RNA PCR Primer, Index 41 (96% over 26bp)
TCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG10.641025641025641TruSeq Adapter, Index 9 (96% over 28bp)
GTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAA10.641025641025641No Hit
CACACCGTCGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT10.641025641025641No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.641025641025641No Hit
ACATCTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGC10.641025641025641Illumina Paired End PCR Primer 2 (95% over 24bp)
CTATTGATCGATATGTATATTTAAATAAATCAAATGTGTAAAATCAACAA10.641025641025641No Hit
CACAGTCTCCCCTGACAGATCGGAAGAGCCCACGAGACCCTAAGACATCT10.641025641025641No Hit
CCCGGCTTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC10.641025641025641No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTCAGACAGAACAAAAACACCCAAA10.641025641025641No Hit
GTTTATGTGATTACAACCATGGAAGCTGGGCATCGCAATGCTGGAAAAAG10.641025641025641No Hit
TCTCCGAGCCCACTAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.641025641025641RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT10.641025641025641Illumina PCR Primer Index 3 (96% over 26bp)
ATACACATCTCCGAGCCCATGAGACATGCGCAGATCTCGTATGCCGTCTT10.641025641025641Illumina PCR Primer Index 9 (95% over 21bp)
GCTCAAAGCCAAGCGCGACTACTTCATTACCGAGGACGGCGGGATCTCCC10.641025641025641No Hit
CCGCGCAGGTGATTCAATTGAACCTCACGTACTCGAAGTCTATCGGCTGA10.641025641025641No Hit
TCATCATATAGACCGATAAGCTTACTAACATGTCTTTCAAAGCTGTTTGA10.641025641025641No Hit
ATTTCGCATATCGCCAGCAAATGCGACATATATACGCAAATTTTGTTTCT10.641025641025641No Hit
CGTCTCCCTGCGGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGT10.641025641025641No Hit
GCTCGGAGATGTGTATAAGAGACAGCACATCTCCGAGCCCACGAGACATG10.641025641025641No Hit
TCTCCGAGCCCACCGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT10.641025641025641Illumina Paired End PCR Primer 2 (96% over 26bp)
AAACTGGACTTGGGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGA10.641025641025641No Hit
TCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG10.641025641025641TruSeq Adapter, Index 9 (96% over 28bp)
CCCCGCGTTAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGC10.641025641025641No Hit
TCTCCGAGTCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.641025641025641RNA PCR Primer, Index 41 (96% over 29bp)
GGTCTGCGCCGATTCGCCGAGCCCGAGACCCTAAGACATCTCGTATGCCG10.641025641025641No Hit
AGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA10.641025641025641RNA PCR Primer, Index 47 (96% over 29bp)
GGGCAAATCTCTGGAATTTCACGCGTTTCACTTAAGCATGTTCACATTTG10.641025641025641No Hit
CACCCCCGTGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT10.641025641025641No Hit
TCTCCGAGCCCACGATACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.641025641025641Illumina PCR Primer Index 3 (96% over 28bp)
CTTAACGACTTCCCCTATTCTTCCCCTACGCCCGCTTACGCGGTCTCCAC10.641025641025641No Hit
CGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAGCCCCAAGTT10.641025641025641No Hit
CCTGACCGTTGGCGATGGTGCCGGCGGTGGCCAGTGAGGCGTGCATGCTG10.641025641025641No Hit
GAGCGGGCTGAGCGGGTCTTTAACCTCCTTCTCTGAGGATGAGCCAGGAT10.641025641025641No Hit
ATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT10.641025641025641TruSeq Adapter, Index 9 (95% over 21bp)
TGCGATGGGTGTCTACCTGGACATGGTCAACAACACCAAGGCGGATCCCG10.641025641025641No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAATACTAATGCC10.641025641025641No Hit
CTTATACACATATCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10.641025641025641No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG10.641025641025641No Hit
CATCTCAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG10.641025641025641TruSeq Adapter, Index 9 (96% over 28bp)
TCCCCTGGCTTTGGTCTAAACTCTTTTGCATTTATTTATGCACTTTGCCG10.641025641025641No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCCCCA10.641025641025641No Hit
ACACATCTCCGGGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT10.641025641025641Illumina Paired End PCR Primer 2 (95% over 22bp)
CCCCAGCCGAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGC10.641025641025641No Hit
GAACGGCAAGGTGCCGAGTAGAGCATATAGTAGAAGTCCAACATGATTAC10.641025641025641No Hit
CTTATACACATCTCCGAGCCCACGAACATGCGCAGATCTCGTATGCCGTC10.641025641025641No Hit
ACCCAGGGGTCGCGGGGGTTGATGCACCGGACGAATTGCCCCTCCGGGTG10.641025641025641No Hit
AACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGC10.641025641025641No Hit
GGCTCGGAGATGTGTATAAGAGCTCCGAGCACACGAGACATGCGCAGATC10.641025641025641No Hit
CTCCAGAGGATGGACCGCCTCCCGCTCAAAGAGGGGTGGCCCGGGGCCAC10.641025641025641No Hit
TCTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.641025641025641TruSeq Adapter, Index 3 (96% over 28bp)
GGGTGGCGGTCAGCTCGACAGTGGTGAGCTGCGTACTGCCATCGACCGTG10.641025641025641No Hit
CAGCATATGCTGCTGAACAAGAAACAGTAGAAAAAGAATATGATGCTCAA10.641025641025641No Hit
CTTATACACATCTCCGAGCACACGAGACATGCGCAGATCTCGTATGCCGT10.641025641025641No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA10.641025641025641No Hit
GATCGGAAGAGCCCCTCGCTAGATCGGAAGAGCCCACGAGACCCTAAGAC10.641025641025641No Hit
GGCAGTATGAGAGGAACGCGGCCAGCACGGTTTCAAAGACGAGGCCAAAG10.641025641025641No Hit
CACCCCTATGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT10.641025641025641No Hit
CCGGATCACCCACCACGTGGGCGCCCATCAGGTCTCAGGCATACAGCCGG10.641025641025641No Hit
GCCCCAGTCAACTACCCATCAAGCACTGTTCCTGGTGGTCTGCACCGGGT10.641025641025641No Hit
CCACTGGACTCTCAATGGACAGCCGATAACGAACGAGCACGTCGGTATAC10.641025641025641No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGAAGCCCAGTTAAACGGCGGCCGT10.641025641025641No Hit
TCTCCGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.641025641025641Illumina PCR Primer Index 3 (96% over 28bp)
GCACTCGTCCTTGGCCAGCAGCTCGGTGACCTCTTCGCCTGTCTCTTATA10.641025641025641No Hit
GAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA10.641025641025641Illumina PCR Primer Index 9 (100% over 27bp)
CGTCCCCGGCCTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.641025641025641No Hit
CTTATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGT10.641025641025641No Hit
CTCCAAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA10.641025641025641RNA PCR Primer, Index 17 (96% over 28bp)
CTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA10.641025641025641Illumina Paired End PCR Primer 2 (96% over 27bp)
TATCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.641025641025641Illumina PCR Primer Index 3 (96% over 28bp)
ATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGT10.641025641025641No Hit
TCTCCGAGCCCACGGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA10.641025641025641RNA PCR Primer, Index 17 (96% over 28bp)
CCCCAGTGAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC10.641025641025641No Hit
TACACATTCCGAGCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG10.641025641025641RNA PCR Primer, Index 47 (96% over 25bp)
GAGAAGATCTTGTAGGGTGTCTCTGGTGGCACGAGCTCTAAGATATCTTG10.641025641025641No Hit
CCCCCCCGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC10.641025641025641No Hit
ATATCATATAAATGAACTGATTGTGTTCTGTTACCATGCAGTTACAAACC10.641025641025641No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACCCACA10.641025641025641No Hit
TTATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTC10.641025641025641No Hit
CTTTGGGAGTTCAATGAGCATTCTTGGAGTTATTAGGTCGTAGCCAGGCG10.641025641025641No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCGACTACT10.641025641025641No Hit
CAAAAAAAAACTTGGCCAACAGGAGCGGCAACCAGCTACGCGGACGAAAA10.641025641025641No Hit
ATCAACGATCCCGAGACAAAGAAGGAGATCGACAATCTGCAGTTCCCAAA10.641025641025641No Hit
CTCCGAACCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA10.641025641025641Illumina PCR Primer Index 9 (96% over 28bp)
TCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAAAAAAAAAAAA10.641025641025641No Hit
GCACATGTCTCCCGTGTGAGATCGGAAGAGCCCACGAGACATGCGCAGAT10.641025641025641No Hit
TCTCAAGATTCCTCCGCGCGCAGCGGATAGGGACCGAACTGTCTCACGAC10.641025641025641No Hit
CAATAATCCCTTCCTTAATGGCTTCCAGCACCGGTCCCCCAAAGGCCCAG10.641025641025641No Hit
GAGCACCCCACCGTAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCG10.641025641025641No Hit
CCCCCCCGGCTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT10.641025641025641No Hit
GCACCCAGTTATAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.641025641025641No Hit
TTCGTGGGAAGCCCTGAGGCCCGGCGTATCTTCCAGCGATACGGTTTCCT10.641025641025641No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGAAAGCCCCCCCGGGGG10.641025641025641No Hit
GACGCGTTATGCGCATTTCTTCATTTTTCATGCATGCTAATGTGTAATAT10.641025641025641No Hit
CCCCGGCATAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC10.641025641025641No Hit
TTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCAAATAG10.641025641025641No Hit
CTGAGAGAGCCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTT10.641025641025641No Hit
ATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAACTGTCTC10.641025641025641No Hit
GCACCCCTGTTGAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTA10.641025641025641No Hit
TCTCCGATCCCACGAGACCCAAAGACATCTCGTATGCCGTCTTCTGCTTG10.641025641025641TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACTATACCCTAAGACATCTCGTATGCCGTCTT10.641025641025641Illumina PCR Primer Index 3 (95% over 21bp)
CATCTCCGAGCCCGCGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT10.641025641025641RNA PCR Primer, Index 47 (96% over 27bp)
CCCCCTCCGAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATG10.641025641025641No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT10.641025641025641No Hit
NTTATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10.641025641025641No Hit
TTATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC10.641025641025641No Hit
CATCTCGTGCCCACGAGACCCTAAGACACCTCGTATGCCGTCTTCTGCTT10.641025641025641Illumina PCR Primer Index 11 (96% over 25bp)
CTTCCGATCTTTGCCGGGGAATCCCCTCCGCCGAAAGCCTAAAGTTTCCT10.641025641025641No Hit
AAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT10.641025641025641No Hit
GAGGTGTTCCGAGCCAAAATGCTGCAAATTCTGCGCCACCTGCCTCAGGA10.641025641025641No Hit
GCAGGGGGAAGAAGCCTGCTTCCAAGGGCCCTTTCGGCTTGGGGTCATTC10.641025641025641No Hit
TCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG10.641025641025641Illumina PCR Primer Index 9 (96% over 28bp)
GAAATCTGCCGGCTGGGCCGGCTGTTTCCCAACCTCGAGGCCCTCGTTCT10.641025641025641No Hit
GCCATTAGCAGGCTCAGCTGGGCGTCCATGCTGGTGTTACCGATAAAACT10.641025641025641No Hit
GCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTCGA10.641025641025641No Hit
TCTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG10.641025641025641RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCAAGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG10.641025641025641Illumina PCR Primer Index 3 (96% over 28bp)
CCTCTCAAATTTCGACGCCCACGGCAGATAGGGACGAAACTGTCTCGCGA10.641025641025641No Hit
ACCCAGGGCCCACCACGTGGCGGTACACATAGCACGGCTTGACGCTGTTC10.641025641025641No Hit
TCCCGAGCCCACTAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA10.641025641025641TruSeq Adapter, Index 3 (96% over 28bp)
TCTCCGAGCCCACGAGCATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA10.641025641025641RNA PCR Primer, Index 47 (96% over 29bp)
CATCTCCTAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT10.641025641025641RNA PCR Primer, Index 47 (96% over 27bp)
CTCCGAGCCCACGAACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA10.641025641025641Illumina PCR Primer Index 3 (96% over 28bp)
ATCAGATACCGCCCTAGTTCTAACCATAAAAGTAGCTACACCCAATTGCT10.641025641025641No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTCTAAAAAGCCCCCCAGACCACAA10.641025641025641No Hit
ACTCTAGATAACATGCACCTCGTATGCCGTCTTCTGCTTGACAAAAAAAA10.641025641025641Illumina PCR Primer Index 3 (96% over 26bp)
CTCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA10.641025641025641RNA PCR Primer, Index 17 (96% over 28bp)
TTTGTGCGCCACTTTGGTCAGAGTTTCGCGTTACTCTGCCAGGCGCAGGC10.641025641025641No Hit
AGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGA10.641025641025641No Hit
AGATCGGAAGAGCACCCCCGCTCAGATCGGAAGAGCCCACGAGACCCTAA10.641025641025641No Hit
GCACCCCAGAGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.641025641025641No Hit
TATACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT10.641025641025641No Hit
ACGCAGTGCGGCCCAGGTGATTGCGATCCTGATCTGCAAGAGCCATCTGG10.641025641025641No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers