FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s53.351000000d81f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s53.351000000d81f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4017912
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT1778554.426552896131125No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT1333633.319211570586912TruSeq Adapter, Index 7 (95% over 21bp)
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG832622.0722703732685037TruSeq Adapter, Index 7 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT705171.7550658152791796RNA PCR Primer, Index 7 (95% over 23bp)
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCT309250.7696783802134044TruSeq Adapter, Index 7 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC289270.7199510591570946No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA236080.5875688666152967No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA233480.5810978438552163RNA PCR Primer, Index 7 (96% over 28bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT203560.5066313050161377No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTG181850.45259826496946676TruSeq Adapter, Index 7 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCT171060.4257435205151332No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA160810.4002327577109703No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT152640.3798988131148716No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGC86700.21578372049960276RNA PCR Primer, Index 29 (95% over 24bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT58800.14634466857412506No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT42510.10580122212731388No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG41050.102167493962038No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT40330.10037551842847728No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA40260.10020129858493666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA147900.028.253061
CCGATCT147250.027.9992834
TTCCGAT148750.027.8765982
TCCGATC149700.027.6679653
TATGCTT27100.024.3628465
GTATAAG10050.024.103568
ATGCTTG25850.023.1522126
GTATTAT11800.022.1577421
TAGGACT32300.020.881824
CTTGTCT28450.020.7022029
ATCTTTT48400.020.6087237
GCTTGTC30100.020.3563698
TATATGC31750.020.3459383
ATATAAG21300.020.069912
ATATGCT32250.019.735934
CGATCTT72950.019.7287645
GTATAGT26700.019.5851171
ATCTAGG35100.019.5029431
GATCTAG11750.019.4037596
CTAGGAC34450.019.3028453
Produced by FastQC (version 0.11.2)