FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s51.351000000d81a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s51.351000000d81a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA322.3952095808383236Illumina PCR Primer Index 11 (100% over 29bp)
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA251.87125748502994Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG241.7964071856287425RNA PCR Primer, Index 29 (96% over 30bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA211.5718562874251496Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA201.4970059880239521TruSeq Adapter, Index 11 (96% over 30bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA161.1976047904191618RNA PCR Primer, Index 29 (96% over 30bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA100.7485029940119761RNA PCR Primer, Index 40 (96% over 29bp)
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG90.6736526946107785TruSeq Adapter, Index 11 (96% over 30bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA80.5988023952095809RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG70.5239520958083832TruSeq Adapter, Index 11 (96% over 30bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA70.5239520958083832TruSeq Adapter, Index 11 (96% over 30bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTGCAGCT70.5239520958083832No Hit
TCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA70.5239520958083832TruSeq Adapter, Index 11 (96% over 30bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA70.5239520958083832RNA PCR Primer, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC70.5239520958083832RNA PCR Primer, Index 29 (96% over 27bp)
CTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA60.4491017964071856Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.4491017964071856Illumina PCR Primer Index 11 (96% over 30bp)
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.4491017964071856TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG50.37425149700598803TruSeq Adapter, Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC50.37425149700598803RNA PCR Primer, Index 11 (96% over 27bp)
GCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA50.37425149700598803Illumina PCR Primer Index 11 (100% over 29bp)
CATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT50.37425149700598803Illumina PCR Primer Index 11 (96% over 28bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA50.37425149700598803RNA PCR Primer, Index 29 (96% over 30bp)
TATCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.29940119760479045RNA PCR Primer, Index 11 (96% over 30bp)
CATCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT40.29940119760479045RNA PCR Primer, Index 29 (96% over 29bp)
AGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA40.29940119760479045TruSeq Adapter, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT40.29940119760479045No Hit
ACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT40.29940119760479045Illumina PCR Primer Index 11 (100% over 24bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA40.29940119760479045RNA PCR Primer, Index 17 (96% over 28bp)
CTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.29940119760479045Illumina PCR Primer Index 11 (96% over 30bp)
TCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.29940119760479045TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCAACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG40.29940119760479045RNA PCR Primer, Index 40 (96% over 29bp)
CTTATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT40.29940119760479045No Hit
ATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT40.29940119760479045TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT40.29940119760479045No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA30.2245508982035928No Hit
CATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT30.2245508982035928TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.2245508982035928Illumina PCR Primer Index 11 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT30.2245508982035928No Hit
GCTCGGAGATGTGTATAAGATCTCCGAGCCCACGAGACGGAGCTACATCT30.2245508982035928No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC30.2245508982035928RNA PCR Primer, Index 41 (96% over 26bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2245508982035928No Hit
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.2245508982035928TruSeq Adapter, Index 10 (96% over 29bp)
GAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA30.2245508982035928TruSeq Adapter, Index 11 (96% over 30bp)
TCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.2245508982035928Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCG30.2245508982035928No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2245508982035928No Hit
CATCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT30.2245508982035928TruSeq Adapter, Index 11 (96% over 29bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC30.2245508982035928RNA PCR Primer, Index 6 (96% over 25bp)
CTTATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGT30.2245508982035928No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2245508982035928No Hit
CGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA30.2245508982035928TruSeq Adapter, Index 11 (96% over 30bp)
CTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.2245508982035928RNA PCR Primer, Index 10 (96% over 29bp)
TCTCCGAGCCCACGAGAAGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.2245508982035928Illumina PCR Primer Index 11 (96% over 30bp)
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT20.14970059880239522TruSeq Adapter, Index 10 (95% over 22bp)
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT20.14970059880239522Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCAACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCT20.14970059880239522RNA PCR Primer, Index 40 (96% over 27bp)
TCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.14970059880239522Illumina PCR Primer Index 11 (96% over 30bp)
CTTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT20.14970059880239522No Hit
ACACATCTCCGAGCCCAGAGACGGAGCTACATCTCGTATGCCGTCTTCTG20.14970059880239522RNA PCR Primer, Index 11 (96% over 26bp)
TATCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.14970059880239522RNA PCR Primer, Index 29 (96% over 30bp)
CTCCGAGCCCACGAGACTGCAGCTAAACTCGTATGCCGTCTTCTGCTTGA20.14970059880239522Illumina PCR Primer Index 12 (96% over 26bp)
CATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT20.14970059880239522TruSeq Adapter, Index 10 (96% over 27bp)
TCTCCGAGCCCAGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA20.14970059880239522Illumina PCR Primer Index 11 (100% over 29bp)
ATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT20.14970059880239522TruSeq Adapter, Index 11 (96% over 29bp)
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT20.14970059880239522No Hit
CTTATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTC20.14970059880239522Illumina PCR Primer Index 11 (95% over 21bp)
TCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA20.14970059880239522Illumina PCR Primer Index 11 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTCTTC20.14970059880239522Illumina PCR Primer Index 11 (95% over 24bp)
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA20.14970059880239522No Hit
CTCCGAGCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA20.14970059880239522Illumina PCR Primer Index 11 (96% over 30bp)
ATACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTC20.14970059880239522Illumina PCR Primer Index 11 (95% over 24bp)
CCCCATGGCAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGC20.14970059880239522No Hit
TCTCCGAGCCCACGAGAGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA20.14970059880239522Illumina PCR Primer Index 11 (100% over 29bp)
CTCCGAGCCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.14970059880239522RNA PCR Primer, Index 29 (96% over 30bp)
CCGAGCCCACGAGACGGAGCTACAACTCGTATGCCGTCTTCTGCTTGAAA20.14970059880239522Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.14970059880239522TruSeq Adapter, Index 10 (96% over 29bp)
ACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCT20.14970059880239522Illumina PCR Primer Index 11 (96% over 25bp)
TCTCCGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG20.14970059880239522Illumina PCR Primer Index 3 (96% over 28bp)
TCTCCGAGCCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT20.14970059880239522RNA PCR Primer, Index 29 (96% over 29bp)
GATCGGAAGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC20.14970059880239522RNA PCR Primer, Index 10 (96% over 26bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.14970059880239522RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT20.14970059880239522TruSeq Adapter, Index 11 (95% over 23bp)
TCTCCGAGCCCACGATACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG20.14970059880239522Illumina PCR Primer Index 3 (96% over 28bp)
TCTCCGAGCCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT20.14970059880239522Illumina PCR Primer Index 11 (100% over 28bp)
CATCTCCGACCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT20.14970059880239522RNA PCR Primer, Index 29 (96% over 29bp)
TCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT20.14970059880239522Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTT20.14970059880239522Illumina PCR Primer Index 11 (95% over 23bp)
TCTCCGACCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA20.14970059880239522Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGCTTG20.14970059880239522Illumina PCR Primer Index 12 (96% over 26bp)
CCCCTGGGCAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGC20.14970059880239522No Hit
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.14970059880239522Illumina PCR Primer Index 10 (96% over 29bp)
CATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT20.14970059880239522TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT20.14970059880239522RNA PCR Primer, Index 11 (96% over 29bp)
ACACATCTCCGAGCCCACGACGGAGCTACATCTCGTATGCCGTCTTCTGC20.14970059880239522RNA PCR Primer, Index 11 (96% over 27bp)
GAGCCCACGAGACCGATCAGTACCTCGTATGCCGTCTTCTGCTTGAAAAA20.14970059880239522Illumina PCR Primer Index 6 (96% over 25bp)
TCCGAGCCCACGAGACTGCAGCTAATCACGTATGCCGTCTTCTGCTTGAA20.14970059880239522RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGT20.14970059880239522No Hit
ACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTCTG20.14970059880239522Illumina PCR Primer Index 11 (96% over 26bp)
CATCTCCGAGCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT20.14970059880239522RNA PCR Primer, Index 29 (96% over 29bp)
CTCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGCTTGA20.14970059880239522Illumina PCR Primer Index 12 (96% over 26bp)
CATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT20.14970059880239522Illumina PCR Primer Index 11 (96% over 28bp)
CCCCTAGGCAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGC20.14970059880239522No Hit
CATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTCTGCTT20.14970059880239522Illumina PCR Primer Index 11 (96% over 29bp)
GGCTCGGAGATGTGTATAAGAGCTCCGAGCCCACGAGACGGAGCTACATC20.14970059880239522No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGC150.00921297147.516-17
CGGAAGA150.00921297147.514-15
ATCGGAA150.00921297147.512-13
AGCTACA550.002183104221.5909120-21
GGAGCTA550.002183104221.5909118-19
CTACATC550.002183104221.5909122-23
CTTGAAA1051.0061303E-518.09523846-47
TGAAAAA1051.0061303E-518.09523848-49
ATCTCGT1101.50539345E-517.27272826-27
TGCCGTC1101.50539345E-517.27272834-35
TATGCCG1101.50539345E-517.27272832-33
CCGTCTT1101.50539345E-517.27272836-37
TGCTTGA1101.50539345E-517.27272844-45
GTCTTCT1101.50539345E-517.27272838-39
CGTATGC1101.50539345E-517.27272830-31
CTCGTAT1101.50539345E-517.27272828-29
CTTCTGC1101.50539345E-517.27272840-41
TCTGCTT1152.2108577E-516.52173842-43
CACGAGA900.001795243515.83333410-11
ACATCTC950.002579617515.024-25
Produced by FastQC (version 0.11.2)