FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s5.351000000d7ba2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s5.351000000d7ba2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3447694
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT441611.2808851365579428No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT324370.9408317559504991Illumina PCR Primer Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG215100.6238952760888873Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT184790.5359814415084401Illumina PCR Primer Index 10 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC82530.2393773925412174No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT73870.2142591540896611Illumina PCR Primer Index 10 (96% over 26bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT57610.16709719598084982No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA55850.16199233458653814Illumina PCR Primer Index 10 (96% over 28bp)
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCT48110.1395425464092811No Hit
ATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGC46020.13348052350353598No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT45060.13069605365209325No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG43040.12483706500634917Illumina PCR Primer Index 10 (95% over 24bp)
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGT42930.12451801116920469No Hit
GTTATGGCTAGGGGGTCATTGCGACTTACCAAACCATTGCAAACTCCGAA40970.11883305188917577No Hit
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCC39920.11578753798916028No Hit
CCTTAGAATACTCATCTCGCCCACCTGTGTCGGTTTGCGGTACGGTCTCG39880.11567151841201684No Hit
CTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTC38960.11300306813771756No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT38930.11291605345485997No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTA38680.11219093109771344No Hit
TGGTAGGAGAGCGTTCTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCT38160.11068267659484861No Hit
GGTTAATATTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGG37050.10746313332911796No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA36510.10589686903768143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT85800.043.9014
CGATCTT27900.035.0712745
TCCGATC113700.033.4626663
CGATCTG42150.032.5678255
TTCTATA7300.031.2325082
CTTCCGA131550.030.5043871
TTCCGAT133550.029.8405322
GATCTAG11200.029.687156
CGATCTC23450.028.5604325
CGATCTA29850.026.8924835
ATATCAT8700.026.752566
GATCTTT22950.026.0781236
GCTCTTC48200.025.2279193
TGCTCTT48900.025.0610542
TCTATAT10400.024.663173
GATCTGG21350.024.472786
AGTATTG6600.024.4690558
TATAGAA7350.023.2650322
TAGAAGT8000.023.155984
GTATTGG7050.022.90729
Produced by FastQC (version 0.11.2)