FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s49.351000000d8172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s49.351000000d8172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA916.513958482462419Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC241.7179670722977811Illumina PCR Primer Index 10 (96% over 25bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA221.574803149606299Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG201.4316392269148175Illumina PCR Primer Index 10 (96% over 28bp)
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA161.1453113815318539Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG141.0021474588403723Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG141.0021474588403723TruSeq Adapter, Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGCT130.9305654974946312No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA120.8589835361488906Illumina PCR Primer Index 10 (96% over 28bp)
TCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG120.8589835361488906Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG120.8589835361488906TruSeq Adapter, Index 10 (96% over 28bp)
TCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG110.7874015748031495Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTT110.7874015748031495TruSeq Adapter, Index 10 (95% over 21bp)
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA100.7158196134574087Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG100.7158196134574087Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT100.7158196134574087Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG100.7158196134574087Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG90.644237652111668TruSeq Adapter, Index 10 (96% over 28bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA90.644237652111668Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT80.5726556907659269TruSeq Adapter, Index 10 (96% over 26bp)
ATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT70.5010737294201861Illumina PCR Primer Index 10 (95% over 21bp)
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.5010737294201861Illumina PCR Primer Index 10 (96% over 28bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA70.5010737294201861Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT70.5010737294201861Illumina PCR Primer Index 10 (96% over 26bp)
CTTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT60.4294917680744453No Hit
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.4294917680744453Illumina PCR Primer Index 10 (96% over 28bp)
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA60.4294917680744453Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.4294917680744453RNA PCR Primer, Index 10 (96% over 28bp)
ACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT60.4294917680744453Illumina PCR Primer Index 10 (95% over 23bp)
CCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAA50.35790980672870437Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGT50.35790980672870437No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT50.35790980672870437TruSeq Adapter, Index 7 (95% over 21bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA50.35790980672870437Illumina PCR Primer Index 11 (100% over 29bp)
CTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.35790980672870437Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT40.28632784538296346No Hit
ACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT40.28632784538296346Illumina PCR Primer Index 10 (95% over 23bp)
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT40.28632784538296346RNA PCR Primer, Index 29 (96% over 27bp)
CATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.28632784538296346Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.28632784538296346RNA PCR Primer, Index 29 (96% over 28bp)
CTCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTGA40.28632784538296346RNA PCR Primer, Index 12 (96% over 26bp)
TTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTC40.28632784538296346No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT40.28632784538296346No Hit
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA40.28632784538296346Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTT30.21474588403722264RNA PCR Primer, Index 29 (96% over 27bp)
ACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCT30.21474588403722264RNA PCR Primer, Index 10 (95% over 23bp)
TCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.21474588403722264RNA PCR Primer, Index 29 (96% over 28bp)
CATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT30.21474588403722264Illumina PCR Primer Index 10 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT30.21474588403722264No Hit
CACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG30.21474588403722264Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.21474588403722264Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGT30.21474588403722264No Hit
CATCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCT30.21474588403722264TruSeq Adapter, Index 10 (96% over 26bp)
ACACATTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG30.21474588403722264Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.21474588403722264RNA PCR Primer, Index 29 (96% over 28bp)
TCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.21474588403722264RNA PCR Primer, Index 29 (96% over 28bp)
CACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG30.21474588403722264TruSeq Adapter, Index 10 (95% over 24bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.21474588403722264TruSeq Adapter, Index 11 (96% over 30bp)
CTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.21474588403722264RNA PCR Primer, Index 10 (96% over 28bp)
ATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT30.21474588403722264Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCGAGCCCGCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG30.21474588403722264Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.21474588403722264Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG30.21474588403722264TruSeq Adapter, Index 10 (96% over 28bp)
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.21474588403722264TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.21474588403722264Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT30.21474588403722264RNA PCR Primer, Index 29 (96% over 27bp)
AGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA30.21474588403722264Illumina PCR Primer Index 11 (96% over 29bp)
ACACATCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTG20.14316392269148173Illumina PCR Primer Index 10 (95% over 24bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA20.14316392269148173No Hit
CATCTCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTT20.14316392269148173Illumina PCR Primer Index 11 (96% over 28bp)
CCCCGGTTAGATCGGAAGAGCCCACGAGACTACGCTGCATCTCGTATGCC20.14316392269148173No Hit
TCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)
CATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT20.14316392269148173TruSeq Adapter, Index 11 (96% over 28bp)
TCTCGGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT20.14316392269148173RNA PCR Primer, Index 7 (95% over 23bp)
CATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT20.14316392269148173Illumina PCR Primer Index 10 (96% over 26bp)
TCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT20.14316392269148173No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCG20.14316392269148173No Hit
CTTATACACATCTCAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT20.14316392269148173No Hit
CTCCGAGCCCACCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)
CTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTCATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA20.14316392269148173RNA PCR Primer, Index 11 (96% over 30bp)
CTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTGAA20.14316392269148173RNA PCR Primer, Index 29 (96% over 27bp)
CATCTCCGAGGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC20.14316392269148173Illumina PCR Primer Index 10 (96% over 25bp)
CCAAGGACTAACTGGAAAGGCCCTGGGAAGGGGTCAAGGTGGTGGAGCCG20.14316392269148173No Hit
TCTCCGAGCCCACGGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT20.14316392269148173RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173TruSeq Adapter, Index 11 (96% over 29bp)
ACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCT20.14316392269148173RNA PCR Primer, Index 10 (95% over 23bp)
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173TruSeq Adapter, Index 7 (96% over 28bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTACGCT20.14316392269148173No Hit
ATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT20.14316392269148173Illumina PCR Primer Index 10 (95% over 21bp)
ACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC20.14316392269148173RNA PCR Primer, Index 29 (96% over 25bp)
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT20.14316392269148173No Hit
CTTATACACATCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGT20.14316392269148173No Hit
CTTATACACATCTCCGAGCCCAAGAGACACTCGCTAATCTCGTATGCCGT20.14316392269148173No Hit
TCTCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGA20.14316392269148173TruSeq Adapter, Index 12 (96% over 26bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173Illumina PCR Primer Index 11 (96% over 30bp)
TCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.14316392269148173TruSeq Adapter, Index 10 (96% over 28bp)
GCGCGCCACGGCCTTGGCCTGGGCCACATCGCCATGACGCAGCAGCACGG20.14316392269148173No Hit
CTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.14316392269148173RNA PCR Primer, Index 10 (96% over 28bp)
NCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTT20.14316392269148173TruSeq Adapter, Index 10 (95% over 21bp)
TTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173TruSeq Adapter, Index 11 (96% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.14316392269148173No Hit
ATACACATCTCCGAGCCCAAGAGACACTCGCTAATCTCGTATGCCGTCTT20.14316392269148173TruSeq Adapter, Index 10 (95% over 21bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA20.14316392269148173TruSeq Adapter, Index 11 (96% over 30bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT20.14316392269148173No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT20.14316392269148173Illumina PCR Primer Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCT20.14316392269148173Illumina PCR Primer Index 10 (95% over 23bp)
TCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAA20.14316392269148173RNA PCR Primer, Index 29 (96% over 27bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA20.14316392269148173RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTG20.14316392269148173TruSeq Adapter, Index 12 (96% over 26bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATATCGTATGCCGTCTTCTGC20.14316392269148173RNA PCR Primer, Index 12 (95% over 23bp)
TATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.14316392269148173Illumina PCR Primer Index 10 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA308.874468E-563.3333361
TCCGAGC458.958523E-863.3333323
CCGAGCC501.8602441E-757.04
CTCCGAG351.9044499E-454.2857172
CGAGCCC600.002720483831.6666685
ATCTCGT1053.8206235E-720.35714326-27
GCTAATC808.2486164E-417.812522-23
TAATCTC808.2486164E-417.812524-25
CGAGACA808.2486164E-417.81258
AGACACT808.2486164E-417.812514-15
CTCGTAT1201.3855824E-617.812528-29
TGCCGTC1252.0516836E-617.09999834-35
TATGCCG1252.0516836E-617.09999832-33
CCGTCTT1252.0516836E-617.09999836-37
GTCTTCT1252.0516836E-617.09999838-39
CGTATGC1252.0516836E-617.09999830-31
TGAAAAA1252.0516836E-617.09999848-49
ACTCGCT850.001240825716.76470618-19
ACACTCG850.001240825716.76470616-17
TCGCTAA850.001240825716.76470620-21
Produced by FastQC (version 0.11.2)