FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s48.351000000d8159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s48.351000000d8159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT21.7699115044247788RNA PCR Primer, Index 47 (96% over 27bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG21.7699115044247788RNA PCR Primer, Index 47 (96% over 29bp)
TGGCAGAAGATCGCCAACAGTGTGCCGAAGAAGACCAAGGAGGAGTGCCT10.8849557522123894No Hit
TCCTGGAACGGAGCGTCATAGAGGGTGAGAATCCCGTCTTTGACACGGAC10.8849557522123894No Hit
CATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCC10.8849557522123894No Hit
GTTTCAGGGTTATATGAATATCTATAACAACTATTTTAAAGCGCCGTGGA10.8849557522123894No Hit
GTGCTCTTCCGATCTACGACGGGCATCTGTACAGTGAGCCTGGTTGTCAC10.8849557522123894No Hit
CCATAGACATCCACGAGCATGGGATACTTGGTTGCGCCACTTGTGTCCAA10.8849557522123894No Hit
AGTACGATCTGTACAATCTGAACACAAAAGAATTCATCCAGTTGACCATC10.8849557522123894No Hit
TGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGACTGAAGTGGAG10.8849557522123894No Hit
CCGATGCACAGGTGAGCGCGGAGCTAGCCAAACAGCTCCAGGAGCTTACC10.8849557522123894No Hit
AAATCGGATTAGCCTAAAGAAGTAAATATTACAATAATGGCTGTCTCTTA10.8849557522123894No Hit
GCGAAAGCATTTGCCAAAGATGTTTTTAATTAATCAAGAACGAAAGTTAG10.8849557522123894No Hit
GATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATC10.8849557522123894No Hit
CGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAA10.8849557522123894No Hit
AATGCCACAAGCCTCAATAGCAGGTTTAAGAGCCTCGATACGCTCAAAGT10.8849557522123894No Hit
AAGTAAGGCAAGATTTCTTTGAAGGACGCAATTCGTGATATTTGTAAATG10.8849557522123894No Hit
GGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAATGAAGAAATAACATCA10.8849557522123894No Hit
TATCAAGTCAACCCATTATCTGACCACTCACCATTTCTCTGACCACATGA10.8849557522123894No Hit
CATTAGTATTGCGTTGCTAGAGGTGAAATTCTTGGATTGACGCAAGACTA10.8849557522123894No Hit
TTGCATTCATCAAACGCTGAATAGCAAAGCCTCTACGCGATTTCATAGTG10.8849557522123894No Hit
AGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGAT10.8849557522123894No Hit
TTTCCATGCGGTGTACTTTATGCGGACACTTCCTACAGGTAGCGTTGACC10.8849557522123894No Hit
ATCTGTACCACCGCAGTCCCGCTGGAGTCGGTTCGATCACCAAGATCTAC10.8849557522123894No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAAAAACCC10.8849557522123894No Hit
GAAGTAAATAAGAAGAAGTGCTGCAAAATAGAGTAAATAAATTAACCGGC10.8849557522123894No Hit
CCCCGTGTCGGGATTGGGTAATTTGCGCGCCTGCCTTCCTTGGATGTGGT10.8849557522123894No Hit
ATGTAGATGAGGCCTTTCGAGCTAATGACGAGCTAATGAACACATATCAA10.8849557522123894No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT10.8849557522123894RNA PCR Primer, Index 47 (95% over 22bp)
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC10.8849557522123894No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG10.8849557522123894No Hit
GAAGTTTTACTTACAAGTTTACTCTGGTGACAATTTATGCTGTCTCTTAT10.8849557522123894No Hit
TGTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCATC10.8849557522123894No Hit
GAACTGGCTCTGGGCGATGTGATGAACATTCGCAAGCGTAATAAGAAGAT10.8849557522123894No Hit
ACAGAATCGTTAGTTGATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAG10.8849557522123894No Hit
GCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCT10.8849557522123894No Hit
TCTTGATGCTGGCTCCTTCGGCGGCGCGCACCGTCTCCTTCACGCTCTGC10.8849557522123894No Hit
CATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTG10.8849557522123894No Hit
AGAGCCTCGATACGCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAGG10.8849557522123894No Hit
TTGCGGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTAGCGACAGCTT10.8849557522123894No Hit
TACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCC10.8849557522123894No Hit
CTGGTGGGCACCGCCTCATATCCGCCCTCCAGCCGATTGTCGTTGTTGCT10.8849557522123894No Hit
GGATTGACGCAAGACTAACTACTGCGAAAGCATTTGCCAAGGATGTTTTC10.8849557522123894No Hit
GCTGAATGCAGCTAAAACCTGCGCCTAACAAAAGCGATGGTCCACTCTAA10.8849557522123894No Hit
GATCTGAGTTCAAACCGGTGTAAGCCAGGTTGGTTTCTATCTTTAAAAAC10.8849557522123894No Hit
CTATTGGAGCGGCCGATGTCTGATTAGCTAGTTGGCGGGGTAAGAGCCCA10.8849557522123894No Hit
TTATTATTCTTTTGGACGAGAAGGAGCACTCCACATTCGCGCACGCCGGA10.8849557522123894No Hit
GGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGA10.8849557522123894No Hit
TTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTT10.8849557522123894No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACAATTTT10.8849557522123894No Hit
CGCTTACACCGTGAACCCCTGCATCACCCGCACCACCCTGGTGCACAAGT10.8849557522123894No Hit
AGTCAACCGAGCCTTACACGGGATCTACACCGAACTGCCCAGGAGTCCAG10.8849557522123894No Hit
ATTATCGAACTCAACGCCCTGCATACGAAAAGACAGAATCTCTTCCAAGA10.8849557522123894No Hit
GAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAG10.8849557522123894No Hit
AAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCAATGGA10.8849557522123894No Hit
ATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAAGTCCCGCGAGAGA10.8849557522123894No Hit
GATGGAGCTCGTCTCGTGCTTTCCGGTCAGACGACGGGCATTGTCCTGTC10.8849557522123894No Hit
AAGCCTGAATGAGCTTAATAGAGGCCAAAGCGGTCTGGAAACGTACGGAT10.8849557522123894No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT10.8849557522123894RNA PCR Primer, Index 47 (95% over 21bp)
GACCCCTGATGGCAGCCCACAGGAAGGAGCCTGGTCTAGACAGGCTGTGG10.8849557522123894No Hit
ATTTGTAAATGGCATGGAAAGTGCAAAGCTCTGATTAAAAACTGTCTCTT10.8849557522123894No Hit
ATATCTACGCCGTACTGATAAAAGAGTGGCTCCAGCCCGAAGAAATCCAG10.8849557522123894No Hit
GACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTC10.8849557522123894No Hit
CTTATACACATCTCCGAGCCCACGAGAGATGCGCAGATCTCGTATGCCGT10.8849557522123894No Hit
AATACCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAG10.8849557522123894No Hit
TCACAAAGCATCGGCGTCCGTGTCAAAGACGGGATTCTCACCCTCTATGA10.8849557522123894No Hit
CTTCCGATCTACCGGGGGGACAACCCTACGACGAACGCTGTGGAGAACAA10.8849557522123894No Hit
TCTCAAGGTAAAAGTCCTGGTTCCCCGACACACCCAGTAAAGGGCATGCC10.8849557522123894No Hit
CCATTATTGTAATATTTAACTTCTTTAGGCTAATCCGATTTCACTCCTGT10.8849557522123894No Hit
CTTATGTTCGGACCTGGTAAGTTTTCCCGTGTTGGGTCAAATTAAGCCGC10.8849557522123894No Hit
GTTGTCCATCGCCTACGCTTATCGCCTCGGCTTAGGTCCCGACTTACCCA10.8849557522123894No Hit
GTTCTTGTTGTTGTTGTTGGTGTTGTTGTTTATTGTTGCTGATGTTCCTT10.8849557522123894No Hit
GAGGTGGTGGCGGCAACCAGGGCTACAGGTGTGGACCTCCACCATATCCC10.8849557522123894No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGGCC10.8849557522123894No Hit
CTATTTTATTATTCCATGCTAATGTATTCGGGCATAGGCCTGATTTGAAC10.8849557522123894No Hit
GTCCTTTACCAGCTTTAGCCATAGCACCAGAAACAAAACTAGGGACGGCC10.8849557522123894No Hit
GAATTTATATTGGTGGAAATAGTGCAGAATCTAAACTTTTTACATTGAAA10.8849557522123894No Hit
ACTCTTTGAGAGCGCATCACAAAAACGCTTCTCCAGACTACAACTCGGAC10.8849557522123894No Hit
AAAGCGAACCAAACAGGCAAAAAATTTAGGGTCGGCATCATAAGCAATAT10.8849557522123894No Hit
CGCCTACGCTTATCGCCTCGGCTTAGGTCCCGACTTACCCAGAGCGGACG10.8849557522123894No Hit
ATACCATCAGCTTTACCGTCTTTCCAGAAATTGTTCCAAGTATCGGCAAC10.8849557522123894No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT10.8849557522123894No Hit
GGTTAAATTAGATTAGAGTGCTTAGCAGAGTGGCCAGCAAATAGCAAACA10.8849557522123894No Hit
GCTTGTAGTTCTCGTGTTGGGTGCCCTTGTACCAGATCAAAGTGCTTTCC10.8849557522123894No Hit
GGTGAGATCGGAAGAGCACACGTCTCCCGGGTGAGATCGGAAGAGCACAC10.8849557522123894Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTATAACTGTGTATTACTGGGGGGGTTTGTTTAATAATTTTAAACCTCG10.8849557522123894No Hit
TAAACTCCTAAGCAGAAAACCTACCGCGCTTCGCTTGGTCAACCCCTCAG10.8849557522123894No Hit
TCATCATAGGACGCACCCTTGCCCAAGCGCACGGTCAAATCGACCACGGA10.8849557522123894No Hit
CAAATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG10.8849557522123894Illumina PCR Primer Index 9 (100% over 23bp)
ATTTACGTCATTCTTATGGTGAGAGAATCGTGCCAATAATTTGTTCTGCA10.8849557522123894No Hit
CCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTATGGTTAGA10.8849557522123894No Hit
CTCGAACGCAGCACCCGCAAATGCCGACTCATCGCCTGCGGCGACAGGCC10.8849557522123894No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10.8849557522123894No Hit
CTACCATGGTGGCTTCAAGAAGACCGACAAGCACCCGGCCTCCAACTTTG10.8849557522123894No Hit
GATTCTCATAACTGTTTGATTTAAGTTGACACATACAAAATACAAAAGGA10.8849557522123894No Hit
TACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAA10.8849557522123894No Hit
CAGCACGCTCCCAAGCATTAAGCTCAGGAAATGCAGCAGCAAGATAATCA10.8849557522123894No Hit
CACTCGGGCAGCTCTGCTCGTACCCGCTGGCCCTGGTGCGCACTCGGCTA10.8849557522123894No Hit
ACCTTAGTCGTTCGGTGGACGGGATTCTCACCCGTCTTTCGCTACTCACA10.8849557522123894No Hit
GTTTGAAGGCGCTGGAGTATTTCTACTTTCAGCAGAACAACTGGAACTGC10.8849557522123894No Hit
CTTCTGGACGGCGGCCTGCACGAGGGGCAGGTTCTTGCCAAGGAAACACT10.8849557522123894No Hit
TCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAA10.8849557522123894No Hit
AATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTA10.8849557522123894No Hit
GGTTCCTGAATGAATGGGAAGCCTTCAAGAAGGTGATAAGCAGGAGAAAC10.8849557522123894No Hit
TGTTCAAGCACTGCAGGAATGGAGTGTCTCCTTGAAAGACGATAAATCCG10.8849557522123894No Hit
GTGCAAAACTCCCTCCTGCTGACCATTGTTGACGATGATGATCTGTCGAC10.8849557522123894No Hit
GGGTTAGTCGATCCTAAGAGATAGGGAAGCTCCGTTTCAAAGCGCACGAT10.8849557522123894No Hit
GTCAGACTCTGGCCCCGCGGGTCAGCGGATGTGCACTTTTCATCAGACCC10.8849557522123894No Hit
TCCATATGTTGAGATTTCAGCCCCCAAAGTGATGGCATTAGGTGAGGCTT10.8849557522123894No Hit
TCGACGCCATTAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGATGAG10.8849557522123894No Hit
ATATATAAGTTATGTGATGCCATTTTCAGGCCGTTTCTGAAGCTGTCTCT10.8849557522123894No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)