FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s47.351000000d8115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s47.351000000d8115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277084
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT956982.9202180963319826No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT684232.087923287898632RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT364801.1131847703629203RNA PCR Primer, Index 47 (95% over 24bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG345151.0532229262356412RNA PCR Primer, Index 47 (96% over 29bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA280140.8548453442145517No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT247140.7541460640008008No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA201920.6161575351745637No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC198470.605629883152217No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT186530.569195052674878No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT163380.4985529818582618RNA PCR Primer, Index 47 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT107130.3269064814939135RNA PCR Primer, Index 47 (95% over 21bp)
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA83450.2546471192071976RNA PCR Primer, Index 47 (96% over 29bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG80310.24506543012019225TruSeq Adapter, Index 9 (95% over 24bp)
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT53410.16298025927928608No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG50830.15510740646257465No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA47430.1447323291072185No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT46670.1424131941689624No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC44870.13692050615730325No Hit
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC43090.13148884801244032No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA41680.12718624240330734No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT40040.12218179332601788No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT35860.10942655116560943No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG33500.10222502688365633No Hit
AGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGA33370.10182833274948093No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC33300.10161472821569419No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC32960.10057722048015859Illumina Paired End PCR Primer 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA100400.029.166911
CCGATCT100900.028.951394
TTCCGAT103200.028.3521822
TCCGATC104250.028.066623
ATCTTTT42650.022.3845947
GTCCTAG24300.021.5193841
TCTTCCG72750.021.0889515
GATCTAG7500.020.2662876
GTATATA14550.019.9301361
TATGCTT21500.019.8833475
CGATCTT62900.019.7850025
TAGGACT27100.019.6306274
ATATAAG16300.019.5241722
CCTAGAT25600.019.2965133
TCCTAGA28450.019.1999912
TAGATAC25850.019.1098945
GCTCTTC79550.018.9877013
ACGGTCC19400.018.8521659
GTCCTAT3054.5256456E-918.7035851
GATCTTT52850.018.604196
Produced by FastQC (version 0.11.2)