HG5M3BCX2_l01n01_r3zt2s46.351000000d80ed.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l01n01_r3zt2s46.351000000d80ed.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1269
Sequences flagged as poor quality	0
Sequence length	101
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	75	5.91016548463357	No Hit
GAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA	21	1.6548463356973995	Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC	18	1.4184397163120568	RNA PCR Primer, Index 12 (96% over 26bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC	12	0.9456264775413712	RNA PCR Primer, Index 41 (96% over 26bp)
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA	12	0.9456264775413712	Illumina PCR Primer Index 5 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.8668242710795903	No Hit
GATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGC	9	0.7092198581560284	Illumina PCR Primer Index 10 (96% over 26bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.5516154452324665	No Hit
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA	6	0.4728132387706856	TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG	4	0.31520882584712373	Illumina PCR Primer Index 12 (96% over 29bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA	4	0.31520882584712373	RNA PCR Primer, Index 17 (96% over 28bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.31520882584712373	No Hit
AAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT	4	0.31520882584712373	Illumina PCR Primer Index 10 (95% over 24bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.2364066193853428	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	3	0.2364066193853428	No Hit
TCTCCGAGCCCACGATACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG	3	0.2364066193853428	TruSeq Adapter, Index 10 (96% over 29bp)
CCCCTGGTGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC	3	0.2364066193853428	No Hit
TCTCCTAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG	3	0.2364066193853428	TruSeq Adapter, Index 12 (96% over 29bp)
GAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAA	3	0.2364066193853428	Illumina PCR Primer Index 10 (96% over 29bp)
CCTCGACACGCTGGCGGCGATGGCGGCGCAGGGCCGCGACATCAAGCTCG	2	0.15760441292356187	No Hit
ACACATCTCCGAGCCCAGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG	2	0.15760441292356187	RNA PCR Primer, Index 12 (96% over 25bp)
TCCTCGATCTGCGCCCCCTGAAAGACAGCAGCGGCGTGGCCGCCGAAGAC	2	0.15760441292356187	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAA	2	0.15760441292356187	No Hit
GCACCCCGCCTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA	2	0.15760441292356187	No Hit
AATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGC	2	0.15760441292356187	Illumina PCR Primer Index 10 (96% over 26bp)
CCCCGGCTTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC	2	0.15760441292356187	No Hit
ATACACATCTCCGAGCCCACGACCGGAGCCTATCTCGTATGCCGTCTTCT	2	0.15760441292356187	Illumina PCR Primer Index 10 (95% over 24bp)
AAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	2	0.15760441292356187	No Hit
CCCCGGGCGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC	2	0.15760441292356187	No Hit
GGTTGGTGAAGATGCGCTGATGCGACAGTTCCAGGATGTTGACGCGTTGC	2	0.15760441292356187	No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT	2	0.15760441292356187	RNA PCR Primer, Index 47 (95% over 24bp)
TCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG	2	0.15760441292356187	Illumina PCR Primer Index 3 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCCTAAGACACCTCGTATGCCGTCTTCTGC	2	0.15760441292356187	Illumina PCR Primer Index 11 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA	2	0.15760441292356187	No Hit
AGACCAAGTCGTGCATTTCCTGCGTGACCTGGCCCAGCGCGCCCGTCCGT	2	0.15760441292356187	No Hit
TGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATG	2	0.15760441292356187	No Hit
CTTATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGT	2	0.15760441292356187	No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA	2	0.15760441292356187	No Hit
AAAAAAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGC	2	0.15760441292356187	No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAA	2	0.15760441292356187	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.15760441292356187	No Hit
CCCCGGATGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC	2	0.15760441292356187	No Hit
TCTCAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG	2	0.15760441292356187	Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	2	0.15760441292356187	No Hit
AAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT	2	0.15760441292356187	No Hit

Adapter Content

Adapter graph

Kmer Content

No overrepresented Kmers

Summary