FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s43.351000000d8080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s43.351000000d8080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2517
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA973.853794199443782Illumina PCR Primer Index 11 (100% over 29bp)
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA431.7083829956297178Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA331.3110846245530394TruSeq Adapter, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC271.0727056019070322RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG261.0329757647993643Illumina PCR Primer Index 11 (96% over 30bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA190.7548669050456893RNA PCR Primer, Index 40 (96% over 29bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG180.7151370679380215TruSeq Adapter, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC170.6754072308303536RNA PCR Primer, Index 11 (96% over 27bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA160.6356773937226857TruSeq Adapter, Index 11 (96% over 30bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA150.5959475566150179Illumina PCR Primer Index 10 (96% over 29bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA140.5562177195073501RNA PCR Primer, Index 17 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA130.5164878823996821No Hit
TCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG120.47675804529201427TruSeq Adapter, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC120.47675804529201427RNA PCR Primer, Index 6 (96% over 25bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA110.43702820818434646RNA PCR Primer, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC110.43702820818434646RNA PCR Primer, Index 41 (96% over 26bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA100.3972983710766786RNA PCR Primer, Index 11 (96% over 30bp)
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG100.3972983710766786TruSeq Adapter, Index 11 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT100.3972983710766786No Hit
CTTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT80.31783869686134286No Hit
TCTCCGAGACCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG80.31783869686134286Illumina PCR Primer Index 11 (96% over 30bp)
TCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA80.31783869686134286TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG80.31783869686134286TruSeq Adapter, Index 11 (96% over 30bp)
CGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA80.31783869686134286TruSeq Adapter, Index 11 (96% over 30bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA80.31783869686134286RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG70.27810885975367505Illumina PCR Primer Index 11 (96% over 30bp)
ACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT70.27810885975367505Illumina PCR Primer Index 11 (100% over 24bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.27810885975367505RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT70.27810885975367505TruSeq Adapter, Index 11 (95% over 23bp)
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG70.27810885975367505TruSeq Adapter, Index 11 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.27810885975367505No Hit
ACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT60.23837902264600713Illumina PCR Primer Index 11 (100% over 24bp)
GAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA60.23837902264600713TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTT60.23837902264600713Illumina PCR Primer Index 11 (95% over 23bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.23837902264600713No Hit
CATCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT60.23837902264600713TruSeq Adapter, Index 11 (96% over 29bp)
ATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT60.23837902264600713TruSeq Adapter, Index 11 (95% over 23bp)
CTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA60.23837902264600713Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.23837902264600713RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCT60.23837902264600713Illumina PCR Primer Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTC50.1986491855383393Illumina PCR Primer Index 11 (95% over 21bp)
TCTCCGAGCCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT50.1986491855383393Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG50.1986491855383393Illumina PCR Primer Index 3 (96% over 28bp)
TCTCCGAGCCCACAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA50.1986491855383393Illumina PCR Primer Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTC50.1986491855383393Illumina PCR Primer Index 11 (95% over 21bp)
TATCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.1986491855383393RNA PCR Primer, Index 11 (96% over 30bp)
CATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT50.1986491855383393TruSeq Adapter, Index 11 (96% over 28bp)
TTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC40.15891934843067143TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCAGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.15891934843067143Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG40.15891934843067143Illumina PCR Primer Index 3 (96% over 28bp)
CATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT40.15891934843067143Illumina PCR Primer Index 11 (96% over 28bp)
TCTCCAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.15891934843067143RNA PCR Primer, Index 11 (96% over 30bp)
CTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.15891934843067143Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG40.15891934843067143TruSeq Adapter, Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACAGACGGAGCTACATCTCGTATGCCGTCTTC40.15891934843067143Illumina PCR Primer Index 11 (95% over 24bp)
CTTATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGT40.15891934843067143No Hit
GGCTCGGAGATGTGTATAAGAGCTCCGAGCCCACGAGACGGAGCTACATC40.15891934843067143No Hit
TTATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTC30.11918951132300357Illumina PCR Primer Index 11 (95% over 21bp)
CATCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT30.11918951132300357RNA PCR Primer, Index 29 (96% over 29bp)
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT30.11918951132300357No Hit
CTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA30.11918951132300357Illumina PCR Primer Index 11 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT30.11918951132300357No Hit
CTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA30.11918951132300357Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.11918951132300357TruSeq Adapter, Index 10 (96% over 29bp)
CTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.11918951132300357RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTCTTCTG30.11918951132300357Illumina PCR Primer Index 11 (96% over 26bp)
ACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT30.11918951132300357Illumina PCR Primer Index 10 (95% over 24bp)
TCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.11918951132300357Illumina PCR Primer Index 10 (96% over 29bp)
CTCCGAGCCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.11918951132300357Illumina PCR Primer Index 11 (96% over 30bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA30.11918951132300357RNA PCR Primer, Index 29 (96% over 30bp)
TCCGAGCCCACGAGACGGAGCTACACCTCGTATGCCGTCTTCTGCTTGAA30.11918951132300357Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCCCACGGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA30.11918951132300357TruSeq Adapter, Index 11 (96% over 30bp)
CTTATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT30.11918951132300357No Hit
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT30.11918951132300357TruSeq Adapter, Index 10 (95% over 22bp)
CGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.11918951132300357RNA PCR Primer, Index 11 (96% over 30bp)
CATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT30.11918951132300357TruSeq Adapter, Index 10 (96% over 27bp)
TCTCCGAGCCCACAAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.11918951132300357RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACCGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT30.11918951132300357Illumina PCR Primer Index 11 (100% over 28bp)
ATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTCTTC30.11918951132300357Illumina PCR Primer Index 11 (95% over 24bp)
TCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.11918951132300357TruSeq Adapter, Index 10 (96% over 29bp)
ACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCT30.11918951132300357Illumina PCR Primer Index 11 (96% over 25bp)
GCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA30.11918951132300357Illumina PCR Primer Index 11 (100% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11918951132300357No Hit
CCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAA30.11918951132300357RNA PCR Primer, Index 29 (96% over 30bp)
CTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA30.11918951132300357Illumina PCR Primer Index 11 (96% over 30bp)
CCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA30.11918951132300357Illumina PCR Primer Index 11 (100% over 29bp)
ACACATCTCCGAGACCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT30.11918951132300357Illumina PCR Primer Index 11 (96% over 25bp)
ACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT30.11918951132300357Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.11918951132300357RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTCTG30.11918951132300357Illumina PCR Primer Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGACGGAGCTACATCTCGTATGCCGTCT30.11918951132300357TruSeq Adapter, Index 11 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCCG155.865875E-495.02
TATACAC250.004456587657.0000043
TTATACA250.004456587657.0000042
GCACCCC250.004456587657.0000041
TACACAT300.00916992247.55
CTTATAC300.00916992247.51
TCTCCGA600.003249435231.6666681
CTCCGAG650.004810516729.230772
GATCGGA601.4025696E-423.7514-15
TCGGAAG601.4025696E-423.7516-17
GGAAGAG652.4145008E-421.92307718-19
GAAGAGC600.004143179419.79166818-19
CGGAAGA600.004143179419.79166816-17
ATCGGAA600.004143179419.79166814-15
AGATCGG600.004143179419.79166812-13
Produced by FastQC (version 0.11.2)